DODAVANJE NUKLEOTIDA ELIMINIRA OŠTEĆENJE LEUKOCITNE DNA IZAZVANE - T-2 TOKSINOM I DEOXYNIVALENOLOM U BROJLERSKIH PILIĆA

This study shows that Fusarium mycotoxins, T-2 toxin and DON impair the performance of broiler chickens, induce DNA damage in chicken leukocytes and cause oxidative stress in the organism. When considering feed consumption and live weight gain in mycotoxicosis induced with high levels of T-2 toxin and DON present in the feed, dietary nucleotides do not have beneficial effect. The crucial role of nucleotide supplementation in feed is to repair DNA damage in immune cells, which are highly sensitive to mycotoxin action. When broilers are exposed to Fussarium mycotoxins their immune function can be depressed, thus they can be more susceptible to virus or bacterial infections. The addition of dietary nucleotides may increase proliferation of immune cells and optimize the function of the immune system in case of infections.Ovaj rad pokazuje da mikotoksini Fusarium, toksini T-2 i DON kvare proizvodne rezultate u brojlera, izazivaju oštećenje DNK u leukocitima pilića i prouzrokuju oksidacijski stres u organizmu. S obzirom na konzumaciju hrane i prirast žive vage u mikotoksikozi izazvanoj visokim razinama toksina T-2 i DON-a što se nalaze u hrani, dijetalni nukleotidi nemaju blagotvorno djelovanje. Presudna uloga dodavanja nukleotida u hranu je obnoviti gubitak DNK u imunim stanicama koje su vrlo osjetljive na djelovanje mikotoksina. Kad su brojleri izloženi mikotoksinima Fusarium njihova se imuna funkcija može smanjiti pa mogu biti osjetljiviji na virusne ili bakterijske infekcije. Dodavanje dijetalnih nukleotida može povećati razmnožavanje imunih stanica i optimirati funkciju imunog sustava u slučaju infekcije

THE EFFECT OF OLIVE BY PRODUCTS AND THEIR EXTRACTS ON ANTIOXIDATIVE STATUS OF LAYING HENS AND OXIDATIVE STABILITY OF EGGS ENRICHED WITH N-3 FATTY ACIDS

The aim of the study was to assess the effects olive leaves, pulp and their extract supplementation on performance, antioxidant status and oxidative stability of eggs. Oxidative stress was induced by the addition of 6% linseed oil in the feed. 94 individually caged laying hens, 40 weeks old, were included in the study. Animals were divided into 6 groups. The feed of each group was composed of a basic feed, supplemented with: group Cont - no supplement, Vit E - 150 IU of α-tocopherol acetate /kg, Olive L - 1% of olive leaves, Olive Ex - extract from olive leaves, the Pulp group - 1% of dried and ground pulp and Pulp Ex - extract from pulp. Based on the results we found out that supplementation of vitamin E, olive leaves, pulp and their extracts had no effect on the performance of hens and showed neither a lymphocyte DNA damage preventive activity nor influence malondialdehyde (MDA) concentration in plasma. The results suggest that α-tocopherol acetate and olive leaves supplementation had significant effect on the MDA content of the stored eggs. Supplements, except vitamin E had neither influence on antioxidant activity (ACL) in eggs nor on n-3 PUFA in fresh and 40 days stored eggs

Do olive polyphenols negatively affect nutrient digestibility in pigs?

It is widely accepted that olive (Olea europaea L.) polyphenols (OP) have many favourable properties (e.g. antioxidative, antimicrobial, antifungal). OP can improve the growth and nutrient digestibility of animals via affecting digestion and health, even though they could negatively affect the mineral absorption by forming insoluble complexes with minerals in the intestine. In the present study, the effects of a dietary olive leaves extract (OLE) on nutrient digestibility in pigs were studied. Twenty-four weaned castrated male piglets were fed a no supplemented (C) or a supplemented diet with three different levels of OLE (O1, O2 and O3: 3.84 mg, 38.4 mg, 96 mg hydroxytyrosol equivalents/day, respectively). Retention and apparent total tract digestibility (ATTD) of dry matter (DM), crude protein (CP), crude fat (CF), organic matter (OM), nitrogen free extract (NFE), ash, neutral (NDF) and acid detergent fibres (ADF), gross energy (GE), Ca, P, Mg, Fe, Cu, Zn, K and Na were measured. Results showed that OLE lowered the retention of Fe and K, and revealed a numerical tendency to lower the retention of Cu and ATTD of Fe and Cu. In conclusion, a low dietary supplementation of OLE has no effect on the digestibility and retention of major nutrients, although some minor negative effects on mineral digestibility can be present

Oštećenje grudne kosti kod kokoši nosilja gajenih u različitim sistemima držanja u Srbiji

The European Union in 2012 banned conventional battery cages for the welfare reasons. However, transition to new housing systems uncovered some new problems, such as keel bone damage (KBD), which also could endanger welfare of laying hens. Although KBD is a research topic which attracts a growing attention in the EU, in Serbia it is still rather unknown phenomenon, even among the scientific and professional community. This research is the first attempt to determine the prevalence of KBD in laying hens in housing systems currently existing in Serbia. The results of conducted monitoring show presence of KBD on all observed farms, except the organic one. The occurrence of KBD was at an acceptable level (from the standpoint of hen welfare) in the free-range system, enriched cages without equipment and conventional battery cages (4%, 3% and 1%, respectively), while in the fully equipped enriched cages it was high (39%). One could assume that this high prevalence of KBD in this system is a consequence of a long roosting on a metal perches.Evropska unija je, zbog obezbeđenja dobrobiti živine, 2012. godine zabranila držanje nosilja u baterijskim kavezima. Međutim, prelazak na nove sisteme držanja doveo je i do nekih novih problema, kakav je oštećenje grudne kosti (OGK), koje takođe može da ugrozi dobrobit živine. Iako je oštećenje grudne kosti istraživačka tema koja u EU privlači sve veću pažnju, u Srbiji je ovo još uvek relativno nepoznat pojam, čak i u naučnim i stručnim krugovima. Ovo istraživanje predstavlja prvi pokušaj da se u Srbiji utvrdi prisustvo oštećenje grudne kosti kod kokošaka nosilja gajenih u različitim sistemima. Dobijeni rezultati pokazuju da je oštećenje grudne kosti prisutno u svim ispitivanim sistemima držanja, osim u organskom. Relativno nizak procenat oštećenja grudne kosti detektovan je kod kokošaka na ispustu, kokošaka u obogaćenim kavezima bez opreme i u konvencionalnim kavezima (4,3 i 1% respektivno). Najveći procenat oštećenja grudne kosti detektovan je kod kokošaka u obogaćenim kavezima sa kompletnom opremom (39%). Može se pretpostaviti da je ovako visok procenat oštećenja grudne kosti u ovom sistemu držanja posledica dugog sedenja kokošaka na metalnim sedalima

Keel bone damage in laying hens reared in different production systems in Serbia

The European Union in 2012 banned conventional battery cages for the welfare reasons. However, transition to new housing systems uncovered some new problems, such as keel bone damage (KBD), which also could endanger welfare of laying hens. Although KBD is a research topic which attracts a growing attention in the EU, in Serbia it is still rather unknown phenomenon, even among the scientific and professional community. This research is the first attempt to determine the prevalence of KBD in laying hens in housing systems currently existing in Serbia. The results of conducted monitoring show presence of KBD on all observed farms, except the organic one. The occurrence of KBD was at an acceptable level (from the standpoint of hen welfare) in the free-range system, enriched cages without equipment and conventional battery cages (4%, 3% and 1%, respectively), while in the fully equipped enriched cages it was high (39%). One could assume that this high prevalence of KBD in this system is a consequence of a long roosting on a metal perches

Evaluation of antioxidant status and lipid peroxidation in dogs with myxomatous mitral valve degeneration stage B1

Myxomatous mitral valve degeneration (MMVD) is the most common naturally occurring heart disease in dogs. There is a lack of data on antioxidant status and oxidative damage in dogs with MMVD stage B1 according to the American College of Veterinary Internal Medicine (ACVIM B1). The aim of this study was to investigate antioxidant status (plasma vitamin E, lipid-standardized vitamin E (LS-VitE), antioxidant capacity of lipid-(ACL) and water-soluble antioxidants, whole blood glutathione peroxidase and erythrocyte superoxide dismutase), and lipid peroxidation [malondialdehyde (MDA)] in dogs with MMVD ACVIM B1. Serum cholesterol and triglyceride concentrations were measured to calculate LS-VitE. Fourteen dogs with MMVD ACVIM B1 and 12 control dogs were included in the study. Dogs with MMVD had significantly higher vitamin E, ACL, MDA, and cholesterol concentrations and significantly higher LS-VitE values than control dogs. No significant correlations between MDA and antioxidant parameters were determined in either group. In conclusion, oxidative damage to lipids is already present and the antioxidant status is altered but not depleted in dogs with MMVD ACVIM B1. The antioxidant response to increased oxidative damage consists mainly of the activation of fat-soluble antioxidants. Further research is needed to evaluate the efficacy and targets of early antioxidant supplementation to prevent or ameliorate oxidative stress and mitigate disease progression in dogs with early-stage MMVD

How Can We Advance Integrative Biology Research in Animal Science in 21st Century?:Experience at University of Ljubljana from 2002 to 2022

In this perspective analysis, we strive to answer the following question: how can we advance integrative biology research in the 21st century with lessons from animal science? At the University of Ljubljana, Biotechnical Faculty, Department of Animal Science, we share here our three lessons learned in the two decades from 2002 to 2022 that we believe could inform integrative biology, systems science, and animal science scholarship in other countries and geographies. Cultivating multiomics knowledge through a conceptual lens of integrative biology is crucial for life sciences research that can stand the test of diverse biological, clinical, and ecological contexts. Moreover, in an era of the current COVID-19 pandemic, animal nutrition and animal science, and the study of their interactions with human health (and vice versa) through integrative biology approaches hold enormous prospects and significance for systems medicine and ecosystem health

Effect of the supplementation of olive leaves and olive cake on growth performance and bone mineralisation of broiler chickens

Submitted 2020-07-01 | Accepted 2020-09-01 | Available 2020-12-01https://doi.org/10.15414/afz.2020.23.mi-fpap.105-111The by-products of olive oil production are not traditional feed ingredients in poultry nutrition. Despite their low nutritional value, they can be used not only as a source of energy and nutrients, but also as bioactive substances in nutrition of broiler chickens. The aim of the study was to determine whether the use of olive leaves and cake in feed mixtures for broilers affects their growth performance and bone mineralisation. In the feeding experiment 120 male Ross 308 broilers were used. At the age of 21 days the chickens were randomly assigned to 5 experimental groups each with two pens (replicates) of 12 animals and fed with 5 different feed mixtures without (Control) or supplemented with 5% or 10% olive leaves (OLeave5, OLeave10) or cake (OCake5, OCake10). Live weight and feed intake per pen were recorded weekly. At the end of the feeding experiment (42 days), 12 chickens per group were randomly selected and slaughtered. At the slaughter line, bone samples (femur, tibia and humerus) were taken from each chicken. The supplementation of olive leaves and cake had no significant effect on the growth performance and mineral content in femur, tibia and humerus of the broilers. The addition of 5 and 10% olive leaves to the feed resulted in higher Cu content in the humerus, but bone mineralisation did not change. In conclusion olive leaves and cake did not modify bone mineralisation but could be supplemented in the feed mixtures for broilers without negative effects on growth performance.Keywords: broiler, olive by-product, live weight, bone mineralisationReferencesAL-HARTHI, M. (2017). The effect of olive cake, with or without enzymes supplementation, on growth performance, carcass characteristics, lymphoid organs and lipid metabolism of broiler chickens. Brazilian Journal of Poultry Science, 19 (spe), 83-90. https://dx.doi.org/10.1590/1806-9061-2016-0311AVIAGEN. (2014). ROSS 308 broiler: Nutrition Specifications. Retrieved June 5, 2020 from http://tmea.staging.aviagen.com/assets/Tech_Center/Ross_Broiler/Ross-308-Broiler-Nutrition-Specs-2014r17-EN.pdfAVIAGEN. (2018). ROSS 308 broiler: Management Handbook. Retrieved July 28, 2020 from http://en.aviagen.com/assets/Tech_Center/Ross_Broiler/Ross-BroilerHandbook2018-EN.pdfBRANCIARI, R. et al. (2017). Oxidative status and presence of Bboactive compounds in meat from chickens fed polyphenols extracted from olive oil industry waste. Sustainability, 9(9), 1566. https://doi.org/10.3390/su9091566ĐUDARIĆ, L. et al. (2015). The role of polyphenols on bone metabolism in osteoporosis. Food Research International, 77(2), 290-298. https://doi.org/10.1016/j.foodres.2015.10.017FLEMING, R. H. (2008). Nutritional factors affecting poultry bone health: Symposium on ‘Diet and bone health’. Proceedings of the Nutrition Society, 67(2), 177-183. https://doi.org/10.1017/S0029665108007015GARCÍA-MARTÍNEZ, O. et al. (2014). The effect of olive oil on osteoporosis prevention. International Journal of Food Sciences and Nutrition, 65(7), 834-840. https://doi.org/10.3109/09637486.2014.931361GHANBARI, R. et al. (2012). Valuable nutrients and functional bioactives in different parts of olive (Olea europaea L.) - a review. International Journal of Molecular Sciences, 13(3), 3291-3340. https://dx.doi.org/10.3390/ijms13033291HAGIWARA, K. et al. (2011). Olive polyphenol hydroxytyrosol prevents bone loss. European Journal of Pharmacology, 662(1-3), 78-84. https://doi.org/10.1016/j.ejphar.2011.04.023HERRERO-ENCINAS, J. (2020). Effects of a bioactive olive pomace extract from Olea europaea on growth performance, gut function, and intestinal microbiota in broiler chickens. Poultry Science, 99(1), 2-10. http://dx.doi.org/10.3382/ps/pez467HILL, F. W. and ANDERSON, D. L. (1958). Comparison of metabolizable energy and productive energy determinations with growing chicks. Journal of Nutrition, 64(4), 587-603. https://doi.org/10.1093/jn/64.4.587IDRUS, R. B. H. and SAIM, A. B. (2019). Olea europaea and its constituents promote bone health by enhancing osteoblast differentiation and proliferation: a review. Pharmacognosy Journal, 11(1), 01-07. https://doi.org/10.5530/pj.2019.1.1LESKOVEC, J. et al. (2018). Effects of olive leaf and marigold extracts on the utilization of nutrients and on bone mineralization using two different oil sources in broilers. Japan Poultry Science Association, 55(1), 17-27. https://doi.org/10.2141/jpsa.0170059LIU, D. et al. (2003). Long-term supplementation of various dietary lipids alters bone mineral content, mechanical properties and histological characteristics of Japanese quail. Poultry Science, 82(5), 831–839. https://doi.org/10.1093/ps/82.5.831MELGUIZO-RODRIGUEZ, L. et al. (2018). Effect of olive oil phenolic compounds on osteoblast differentiation. European Journal of Clinical Investigation, 48(4), e12904. https://doi.org/10.1111/eci.12904MOLINA-ALCAIDE, E. and YANEZ-RUIZ, D. R. (2008). Potential use of olive byproducts in ruminant feeding: A review. Animal Feed Science and Technology, 147(1-3), 247-264. https://doi.org/10.1016/j.anifeedsci.2007.09.021NAUMANN, C. and BASSLER, R. (1997). Methodenbuch Band III: Die Chemische Untersuchung von Futtermitteln. VDLUFA-Verlag: Darmstadt, Germany.NUNES, M. A. et al. (2016). Olive by-products for functional and food applications: Challenging opportunities to face environmental constraints. Innovative Food Science and Emerging Technologies, 35, 139-148. https://doi.org/10.1016/j.ifset.2016.04.016RATH, N. C. et al. (2000). Factors regulating bone maturity and strength in poultry. Poultry Science, 79 (7), 1024-1032. https://doi.org/10.1093/ps/79.7.1024ŞAHIN, S. and BILGIN, M. (2018). Olive tree (Olea europaea L.) leaf as a waste by-product of table olive and olive oil industry: a review. Journal of the Science of Food and Agriculture, 98(4), 1271-1279. https://doi.org/10.1002/jsfa.8619SALOBIR, J. et al. (2013). The use of olive by-products in nutrition of laying hens. Čeh, T. & Kapun, S. (Eds.) The 22th International Scientific Symposium on Nutrition of Farm Animals Zadravec-Erjavec Days 2013. Murska Sobota, Chamber of Agriculture and Foresty of Slovenia (pp. 107-112).SANDBERG, A. S. (2002). Bioavailability of minerals in legumes. British Journal of Nutrition, 88(3), 281-285. https://doi.org/10.1079/bjn/2002718SANTIAGO-MORA, R. et al. (2011). Oleuropein enhances osteoblastogenesis and inhibits adipogenesis: the effect on differentiation in stem cells derived from bone marrow. Osteoporosis International, 22(2), 675-684. https://doi.org/10.1007/s00198-010-1270-xSAYEHBAN, P. et al. (2016). Effects of different levels of two types of olive pulp with or without exogenous enzyme supplementation on broiler performance and economic parameters. Brazilian Journal of Poultry Science, 18(3), 489-500. http://dx.doi.org/10.1590/1806-9061-2015-0060SUCHÝ, P. et al. (2009). Chemical composition of bone tissue in broiler chickens intended for slaughter. Czech Journal of Animal Science, 54(7), 324-330. https://doi.org/10.17221/1726-CJASSURAI, P. F. (2014). Polyphenol compounds in the chicken/animal diet: from the past to the future. Journal of Animal Physiology and Animal Nutrition, 98(1), 19-31. https://doi.org/10.1111/jpn.12070TALATY, P. N. et al. (2009). Life cycle changes in bone mineralization and bone size traits of commercial broilers. Poultry Science, 88(5), 1070-1077. https://doi.org/10.3382/ps.2008-00418VARMAGHANY, et al. (2013). Effect of olive leaves on ascites incidence, hematological parameters and growth performance in broilers reared under standard and cold temperature conditions. Animal Feed Science and Technology, 185(1-2), 60-69. https://doi.org/10.1016/j.anifeedsci.2013.07.002VENÄLÄINEN, E. et al. (2006). Effects of dietary metabolisable energy, calcium and phosphorus on bone mineralisation, leg weakness and performance of broiler chickens. British Poultry Science, 47(3), 301-310. https://doi.org/10.1080/00071660600741776YANG, C. S. and LANDAU, J. M. (2000). Effects of tea consumption on nutrition and health. The Journal of Nutrition, 130(10), 2409-2412. https://doi.org/10.1093/jn/130.10.2409

Breast muscle abnormalities in broiler chickens

V svetovnem merilu perutninsko meso predstavlja cenovno dostopen in kakovosten vir beljakovin, zato se je v zadnjih desetletjih povečala njegova prireja. Slednje smo dosegli z intenzivno selekcijo pitovnih piščancev na hitro rast, učinkovitejše izkoriščanje krme, povečan delež prsne mišičnine in manjši delež trebušne maščobe. Na drugi strani so tako intenzivna selekcija in zahteve po večji prireji, kot tudi nekateri zunanji stresni dejavniki, ki vključujejo neželene spremembe v okolju, prehranskem režimu, tehnologiji reje in nepravilne postopke ravnanja z živalmi pred zakolom, povzročili večjo dovzetnost živali za oksidativni stres, ki poslabšuje senzorične in tehnološke lastnosti perutninskega mesa. Posledice hitrega povečevanja prsne mišičnine pri pitovnih piščancih in intenzivne tehnologije reje se kažejo v naraščajočem trendu pojavnosti različnih neželenih sprememb piščančjega mesa oziroma miopatij. Med najpogostejše miopatije spadajo miopatija globoke prsne mišice, bledo, mehko in vodeno meso, bela progavost prsne mišice, olesenelost prsne mišičnine ter špagetasto meso, ki prizadenejo predvsem veliko prsno mišico in poslabšujejo njene senzorične in tehnološke lastnosti. Prav tako omenjene spremembe prsne mišičnine poslabšujejo kakovost in prehransko vrednost mesa ter vplivajo na sprejemljivost mesa za potrošnike in nedvomno povzročajo ekonomske izgube v živilsko-predelovalni industriji.In recent decades, global production of poultry meat has increased due to its affordable prices and good nutritional value. The latter has been achieved by intensive selection of broilers for increased growth rate, feed efficiency, breast yield and reduced abdominal fat deposition. On the other hand, intensive selection and increasing demand for poultry meat, as well as some environmental stressors, such as changes in environmental temperature, feeding regime, breeding technology, and improper handling procedures before slaughter, lead to increased susceptibility of animals to oxidative stress, resulting in poorer sensory and technological characteristics of chicken meat. As a result of intensive broiler production and the increase in breast muscle, various breast muscle abnormalities or myopathies have been observed. The most common ones include deep pectoral myopathy, pale, soft and exudative like meat, white striping, wooden-breast and spaghetti meat, which mainly affect the pectoralis major breast muscle and negatively influence the sensory and technological characteristics of breast meat. The muscle abnormalities have a detrimental effect on quality and nutritional value of meat, affect consumer compromise consumers acceptance, and cause economic losses in the meat processing industry