Digitale Transformationen der Gesellschaft. Sozialethische Perspektiven auf den technologischen Wandel

Kistler, Sebastian/Puzio, Anna/Riedl, Anna-Maria/Veith (Hrsg.) Digitale Transformationen der Gesellschaft Sozialethische Perspektiven auf den technologischen Wandel Die Digitalisierung bewirkt Transformationsprozesse, die die Formen unseres Zusammenlebens grundlegend verändern. Dies betrifft nicht nur die Art, wie wir leben, Partner suchen, arbeiten, wohnen, konsumieren oder uns selbst präsentieren – auch die gesellschaftlichen Lebensbereiche wie Politik, Bildung, Wirtschaft und Gesundheit befinden sich in einem digitalen Wandel. Mit diesen Veränderungsprozessen sind nicht nur Hoffnungen, sondern auch Ängste verbunden, die die Ambivalenzen der Digitalisierung zum Ausdruck bringen. Die Komplexität und die Wirkungstiefe digitaler Transformationsprozesse werfen Fragen nach deren Steuerbarkeit, nach ihren Zwecken bzw. nach möglichen Orientierungen auf: • Welche technologischen Entwicklungen müssen reguliert werden? • Wie kann eine entsprechende Legitimation erfolgen? • Wer übernimmt Verantwortung? • Wie und nach welchen Kriterien sind ethische Bewertungen vorzunehmen? • Und nicht zuletzt: Gibt es Grenzen für diese Entwicklungen oder haben die Möglichkeiten des Machbaren die ethischen Fragen längst überholt und erledigt? Die Texte des Sammelbandes nähern sich den digitalen Transformationen aus verschiedenen Perspektiven. Anhand unterschiedlicher Zugänge werden die Themenfelder „Digitalisierung und Gesellschaft“, „Körper und Technologie“ sowie „Digitalisierung und Demokratie“ entfaltet. Darüber hinaus werden die Konsequenzen für die Pflegearbeit und für den Umgang mit modernen Waffentechnologien als anwendungsbezogene Konkretionen diskutiert

Asymptomatic infection of the fungal pathogen Batrachochytrium salamandrivorans in captivity

One of the most important factors driving amphibian declines worldwide is the infectious disease, chytridiomycosis. Two fungi have been associated with this disease, Batrachochytrium dendrobatidis and B. salamandrivorans (Bsal). The latter has recently driven Salamandra salamandra populations to extirpation in parts of the Netherlands, and Belgium, and potentially also in Germany. Bsal has been detected in the pet trade, which has been hypothesized to be the pathway by which it reached Europe, and which may continuously contribute to its spread. In the present study, 918 amphibians belonging to 20 captive collections in Germany and Sweden were sampled to explore the extent of Bsal presence in captivity. The fungus was detected by quantitative Polymerase Chain Reaction (qPCR) in ten collections, nine of which lacked clinical symptoms. 23 positives were confirmed by independent processing of duplicate swabs, which were analysed in a separate laboratory, and/or by sequencing ITS and 28 S gene segments. These asymptomatic positives highlight the possibility of Bsal being widespread in captive collections, and is of high conservation concern. This finding may increase the likelihood of the pathogen being introduced from captivity into the wild, and calls for according biosecurity measures. The detection of Bsal-positive alive specimens of the hyper-susceptible fire salamander could indicate the existence of a less aggressive Bsal variant or the importance of environmental conditions for infection progression

The acid ceramidase/ceramide axis controls parasitemia in Plasmodium yoelii-infected mice by regulating erythropoiesis

Acid ceramidase (Ac) is part of the sphingolipid metabolism and responsible for the degradation of ceramide. As bioactive molecule, ceramide is involved in the regulation of many cellular processes. However, the impact of cell-intrinsic Ac activity and ceramide on the course of Plasmodium infection remains elusive. Here, we use Ac-deficient mice with ubiquitously increased ceramide levels to elucidate the role of endogenous Ac activity in a murine malaria model. Interestingly, ablation of Ac leads to alleviated parasitemia associated with decreased T cell responses in the early phase of Plasmodium yoelii infection. Mechanistically, we identified dysregulated erythropoiesis with reduced numbers of reticulocytes, the preferred host cells of P. yoelii, in Ac-deficient mice. Furthermore, we demonstrate that administration of the Ac inhibitor carmofur to wildtype mice has similar effects on P. yoelii infection and erythropoiesis. Notably, therapeutic carmofur treatment after manifestation of P. yoelii infection is efficient in reducing parasitemia. Hence, our results provide evidence for the involvement of Ac and ceramide in controlling P. yoelii infection by regulating red blood cell development

Expanding distribution of lethal amphibian fungus Batrachochytrium salamandrivorans in Europe

Emerging fungal diseases can drive amphibian species to local extinction. During 2010-2016, we examined 1,921 urodeles in 3 European countries. Presence of the chytrid fungus Batrachochytrium salamandrivorans at new locations and in urodeles of different species expands the known geographic and host range of the fungus and underpins its imminent threat to biodiversity

The C-terminal domain of p53 orchestrates the interplay between non-covalent and covalent poly(ADP-ribosyl)ation of p53 by PARP1

The post-translational modification poly(ADPribosyl)ation (PARylation) plays key roles in genome maintenance and transcription. Both non-covalent poly(ADP-ribose) binding and covalent PARylation control protein functions, however, it is unknown how the two modes of modification crosstalk mechanistically. Employing the tumor suppressor p53 as a model substrate, this study provides detailed insights into the interplay between noncovalent and covalent PARylation and unravels its functional significance in the regulation of p53. We reveal that the multifunctional Cterminal domain (CTD) of p53 acts as the central hub in the PARylation-dependent regulation of p53. Specifically, p53 bound to auto-PARylated PARP1 via highly specific non–covalent PAR-CTD interaction, which conveyed target specificity for its covalent PARylation by PARP1. Strikingly, fusing the p53-CTD to a protein that is normally not PARylated, renders this a target for covalent PARylation as well. Functional studies revealed that the p53–PAR interaction had substantial implications on molecular and cellular levels. Thus, PAR significantly influenced the complex p53–DNA binding properties and controlled p53 functions, with major implications on the p53-dependent interactome, transcription, and replication-associated recombination. Remarkably, this mechanism potentially also applies to other PARylation targets, since a bioinformatics analysis revealed that CTD-like regions are highly enriched in the PARylated proteome