Additional file1: of Isoform-specific deletion of PKM2 constrains tumor initiation in a mouse model of soft tissue sarcoma

Figure S1. Quantification of PKM1 and PKM2 staining intensities shown as percent of tissue cores scored in 16 normal human skeletal muscle samples (A) and 48 primary human rhabdomyosarcomas (B). Score 0 = no staining, Score 1 = weak, Score 2 = positive, or Score 3 = strong. (C) Representative images of IHC for PKM1 and PKM2 in KP mouse sarcoma tissue. Corresponding H&E images are shown. Scale bars, 20 μm. (JPG 1314 kb

Additional file 4: of Isoform-specific deletion of PKM2 constrains tumor initiation in a mouse model of soft tissue sarcoma

Table S1. Raw data for LC-MS metabolomics on KP M2+/+ and KP M2-/- sarcomas. Related to Fig. 5. This table contains all the LC-MS metabolomics data. Includes normalization and statistical analysis for each measured metabolite. (XLSX 140 kb

Additional file 3: of Isoform-specific deletion of PKM2 constrains tumor initiation in a mouse model of soft tissue sarcoma

Figure S3. (A) Representative images of IF for tdTomato, PKM1 or PKM2, and EdU on KP M2 +/+ or KP M2 −/− sarcoma cell lines. (B) Cumulative populaion doublings of KP M2 +/+ or KP M2 −/− sarcoma cell lines, n = 3 KP M2 +/+ cell lines and n = 4 KP M2 −/− cell lines. (C) Sarcoma weight in grams. (JPG 2694 kb