Long Live the Image: On Enabling Resilient Production Database Containers for Microservice Applications

[Abstract]: Microservices architecture advocates decentralized data ownership for building software systems. Particularly, in the Database per Service pattern, each microservice is supposed to maintain its own database and to handle the data related to its functionality. When implementing microservices in practice, however, there seems to be a paradox: The de facto technology (i.e., containerization) for microservice implementation is claimed to be unsuitable for the microservice component (i.e., database) in production environments, mainly due to the data persistence issues (e.g., dangling volumes) and security concerns. As a result, the existing discussions generally suggest replacing database containers with cloud database services, while leaving the on-premises microservice implementation out of consideration. After identifying three statelessness-dominant application scenarios, we proposed container-native data persistence as a conditional solution to enable resilient database containers in production. In essence, this data persistence solution distinguishes stateless data access (i.e., reading) from stateful data processing (i.e., creating, updating, and deleting), and thus it aims at the development of stateless microservices for suitable applications. In addition to developing our proposal, this research is particularly focused on its validation, via prototyping the solution and evaluating its performance, and via applying this solution to two real-world microservice applications. From the industrial perspective, the validation results have proved the feasibility, usability, and efficiency of fully containerized microservices for production in applicable situations. From the academic perspective, this research has shed light on the operation-side micro-optimization of individual microservices, which fundamentally expands the scope of “software micro-optimization” and reveals new research opportunities

Case Report on Leiomyosarcoma of the Vulva: A Rare Pathology

Leiomyosarcoma (LMS) of the vulva is rare. However it is the most common histologic subtype of vulvar sarcoma, accounting for approximately 1% of all vulvar malignancies. [1-8] Whether genetics and epigenetics play a role in pathogenesis is unclear. [1] The tumor is slow-growing with non-specific symptoms, has high metastatic potential, and follows a bimodal age distribution. [1-8] Diagnosis and prognosis are based upon immunohistochemical expression and criteria from early literature. [1,3,5-7] The most common therapeutic approach involves radical vulvectomy with lymph node resection. The value of adjuvant chemotherapy and radiation remains unknown. [1,3,5-8] Our case describes a 46-year-old Caucasian G2P2 female with LMS of the left labia

Reutilización de botellas de plástico en la localidad de Saladas

El proyecto de extensión “Construir reciclando”, financiado por la Secretaría de Políticas Universitarias fue realizado por alumnos del Profesorado en Ciencias Químicas y del Ambiente junto a docentes de la FaCENA - UNNE. El mismo estuvo destinado a crear una actitud comprometida con el medio ambiente. El reciclado es un tema que nos afecta a todos por igual. El medio ambiente, los recursos naturales y el ser humano con todas sus actividades están estrechamente relacionados y si alguno de estos componentes influye de manera positiva o negativa sobre el/los otro/s, todo el sistema va a notar ese cambio. Este trabajo se realizó con alumnos y docentes de la Escuela Técnica Dr. Juan Gregorio Pujol, de la localidad de Saladas de la provincia de Corrientes. Las actividades desarrolladas se orientaron hacia la elaboración de ladrillos ecológicos como así también a la confección de “puff ” a partir de botellas de plástico descartable.Fil: Giménez, Andrea F.. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: Sotelo Gasfrascoli, Lucía V.. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: Legizamón, Andrea M.. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: Coronel, Silvia C.. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: López, Romina N.. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: Booth, Yanina M. R.. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: López, Antonella E.. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: Lasala, César G.. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: Peyrano, Felicitas. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Vallejos, Margarita. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Chaves, María Guadalupe. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: Avanza, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; ArgentinaFil: Acevedo, Belén Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentin

Fragmentation of Andes-to-Amazon connectivity by hydropower dams

Andes-to-Amazon river connectivity controls numerous natural and human systems in the greater Amazon. However, it is being rapidly altered by a wave of new hydropower development, the impacts of which have been previously underestimated. We document 142 dams existing or under construction and 160 proposed dams for rivers draining the Andean headwaters of the Amazon. Existing dams have fragmented the tributary networks of six of eight major Andean Amazon river basins. Proposed dams could result in significant losses in river connectivity in river mainstems of five of eight major systems—the Napo, Marañón, Ucayali, Beni, and Mamoré. With a newly reported 671 freshwater fish species inhabiting the Andean headwaters of the Amazon (>500 m), dams threaten previously unrecognized biodiversity, particularly among endemic and migratory species. Because Andean rivers contribute most of the sediment in the mainstem Amazon, losses in river connectivity translate to drastic alteration of river channel and floodplain geomorphology and associated ecosystem services

Reutilización de botellas de plástico en la localidad de Saladas

El proyecto de extensión “Construir reciclando”, financiado por la Secretaría de Políticas Universitarias fue realizado por alumnos del Profesorado en Ciencias Químicas y del Ambiente junto a docentes de la FaCENA - UNNE. El mismo estuvo destinado a crear una actitud comprometida con el medio ambiente. El reciclado es un tema que nos afecta a todos por igual. El medio ambiente, los recursos naturales y el ser humano con todas sus actividades están estrechamente relacionados y si alguno de estos componentes influye de manera positiva o negativa sobre el/los otro/s, todo el sistema va a notar ese cambio. Este trabajo se realizó con alumnos y docentes de la Escuela Técnica Dr. Juan Gregorio Pujol, de la localidad de Saladas de la provincia de Corrientes. Las actividades desarrolladas se orientaron hacia la elaboración de ladrillos ecológicos como así también a la confección de “puff ” a partir de botellas de plástico descartable.

Development and validation of DNA Methylation scores in two European cohorts augment 10-year risk prediction of type 2 diabetes

This is the author accepted manuscriptAvailability of Data and Material: According to the terms of consent for Generation Scotland participants, access to data must be reviewed by the Generation Scotland Access Committee. Applications should be made to [email protected]. All code is available with open access at the following Gitlab repository: https://github.com/marioni-group MethylPipeR (version 1.0.0) is available at: https://github.com/marioni-group/MethylPipeR MethylPipeR-UI is available at: https://github.com/marioni-group/MethylPipeR-UI. The informed consents given by KORA study participants do not cover data posting in public databases. However, data are available upon request from KORA Project Application Self Service Tool (https://epi.helmholtz-muenchen.de/). Data requests can be submitted online and are subject to approval by the KORA Board.Type 2 diabetes mellitus (T2D) presents a major health and economic burden that could be alleviated with improved early prediction and intervention. While standard risk factors have shown good predictive performance, we show that the use of blood-based DNA methylation information leads to a significant improvement in the prediction of 10-year T2D incidence risk. Previous studies have been largely constrained by linear assumptions, the use of CpGs one-at43 a-time, and binary outcomes. We present a flexible approach (via an R package, MethylPipeR) based on a range of linear and tree-ensemble models that incorporate time-to-event data for prediction. Using the Generation Scotland cohort (training set ncases=374, ncontrols=9,461; test set ncases=252, ncontrols=4,526) our best-performing model (Area Under the Curve (AUC)=0.872, Precision Recall AUC (PRAUC)=0.302) showed notable improvement in 10-year onset prediction beyond standard risk factors (AUC=0.839, PRAUC=0.227). Replication was observed in the German-based KORA study (n=1,451, ncases = 142, p=1.6x10-5 49 ).Wellcome TrustChief Scientist Office of the Scottish Government Health DirectoratesScottish Funding Counci

A nationwide pilot study on breast cancer screening in Peru

"Introduction: A high prevalence of advanced breast cancer (BC) is a common scenario in Latin America. In Peru, the frequency of BC at Stages III/IV is ≈50% despite implementation of a programme for breast cancer screening (BCS) along the country. We carried out a study to assess the feasibility and develop an instrument to evaluate the knowledge, barriers and perception about BCS in a nationwide pilot study in Peru among candidates for BCS. Methods: We conducted a systematic review of 2,558 reports indexed in PubMed, Scopus, Web of Science, Medline-Ovid and EMBASE, regarding to our study theme. In total, 111 were selected and a 51-items survey was developed (eight items about sociodemographic characteristics). Patients were recruited in public hospitals or private clinics, in rural and urban areas of nine departments of Peru. Results: We surveyed 488 women from: Lima (150), Cajamarca (93), Ica (59), Arequipa (56), Loreto (48), Ancash (38), Junín (15), Puerto Maldonado (15) and Huancavelica (14); 27.9% of them were from rural areas. The mean of age was 53.3 years (standard deviation ± 9.1). Regarding education level, 29.8% had primary, 33.2% secondary and 37.0% higher education. In total, 28.7% of women did not know the term ‘mammogram’ and 47.1% reported never receiving a BCS (36.9% from urban and 73.5% from rural population). In women that underwent BCS, only 67% knew it is for healthy women. In total, 54.1% of patients had low levels of knowledge about risk factors for BC (i.e. 87.5% of women respond that injuries in the breast produce cancer). Cultural, economic and geographic barriers were significantly associated with having a mammogram where 56.9% of participants considered a cost ≤ 7 USD as appropriate. Mammogram was perceived as too painful for 54.9% of women. In addition, women with a self-perception of low-risk for BC and a fatalistic perception of cancer were less likely to have a BCS. Conclusion: We found that it is feasible to conduct a large-scale study in Peru. The results of this pilot study highlight an urgent need of extensive education and awareness about BCS in Peru.

SEN1990 is a predicted winged helix-turn-helix protein involved in the pathogenicity of Salmonella enterica serovar Enteritidis and the expression of the gene oafB in the SPI-17

Excisable genomic islands (EGIs) are horizontally acquired genetic elements that harbor an array of genes with diverse functions. ROD21 is an EGI found integrated in the chromosome of Salmonella enterica serovar Enteritidis (Salmonella ser. Enteritidis). While this island is known to be involved in the capacity of Salmonella ser. Enteritidis to cross the epithelial barrier and colonize sterile organs, the role of most ROD21 genes remains unknown, and thus, the identification of their function is fundamental to understanding the impact of this EGI on bacterium pathogenicity. Therefore, in this study, we used a bioinformatical approach to evaluate the function of ROD21-encoded genes and delve into the characterization of SEN1990, a gene encoding a putative DNA-binding protein. We characterized the predicted structure of SEN1990, finding that this protein contains a three-stranded winged helix-turn-helix (wHTH) DNA-binding domain. Additionally, we identified homologs of SEN1990 among other members of the EARL EGIs. Furthermore, we deleted SEN1990 in Salmonella ser. Enteritidis, finding no differences in the replication or maintenance of the excised ROD21, contrary to what the previous Refseq annotation of the protein suggests. High-throughput RNA sequencing was carried out to evaluate the effect of the absence of SEN1990 on the bacterium’s global transcription. We found a downregulated expression of oafB, an SPI-17-encoded acetyltransferase involved in O-antigen modification, which was restored when the deletion mutant was complemented ectopically. Additionally, we found that strains lacking SEN1990 had a reduced capacity to colonize sterile organs in mice. Our findings suggest that SEN1990 encodes a wHTH domain-containing protein that modulates the transcription of oafB from the SPI-17, implying a crosstalk between these pathogenicity islands and a possible new role of ROD21 in the pathogenesis of Salmonella ser. Enteritidis

Saturation of an Intra-Gene Pool Linkage Map: Towards a Unified Consensus Linkage Map for Fine Mapping and Synteny Analysis in Common Bean

Map-based cloning and fine mapping to find genes of interest and marker assisted selection (MAS) requires good genetic maps with reproducible markers. In this study, we saturated the linkage map of the intra-gene pool population of common bean DOR364×BAT477 (DB) by evaluating 2,706 molecular markers including SSR, SNP, and gene-based markers. On average the polymorphism rate was 7.7% due to the narrow genetic base between the parents. The DB linkage map consisted of 291 markers with a total map length of 1,788 cM. A consensus map was built using the core mapping populations derived from inter-gene pool crosses: DOR364×G19833 (DG) and BAT93×JALO EEP558 (BJ). The consensus map consisted of a total of 1,010 markers mapped, with a total map length of 2,041 cM across 11 linkage groups. On average, each linkage group on the consensus map contained 91 markers of which 83% were single copy markers. Finally, a synteny analysis was carried out using our highly saturated consensus maps compared with the soybean pseudo-chromosome assembly. A total of 772 marker sequences were compared with the soybean genome. A total of 44 syntenic blocks were identified. The linkage group Pv6 presented the most diverse pattern of synteny with seven syntenic blocks, and Pv9 showed the most consistent relations with soybean with just two syntenic blocks. Additionally, a co-linear analysis using common bean transcript map information against soybean coding sequences (CDS) revealed the relationship with 787 soybean genes. The common bean consensus map has allowed us to map a larger number of markers, to obtain a more complete coverage of the common bean genome. Our results, combined with synteny relationships provide tools to increase marker density in selected genomic regions to identify closely linked polymorphic markers for indirect selection, fine mapping or for positional cloning

Rendimiento de cultivares de arveja (Pisum sativum, L) en diferentes ambientes de la República Argentina - Campaña 2018-2019

Se estima que la población mundial para 2050 llegará a las 9.000 millones de personas, por lo que la demanda de alimentos será creciente y las legumbres juegan un papel fundamental en la dieta de los países que más población tienen, como India y China. Dentro de las legumbres, arveja es la especie de menor precio en relación a lenteja, garbanzo o porotos. En este sentido, cuando se hace necesario importar alimentos, las arvejas son las preferidas. El área de siembra de arveja en Argentina no se ha incrementado, en gran parte por el bajo precio en 2018 en relación a otros cultivos de invierno y, por otro lado, por el escaso o nulo consumo interno (Vita y Prieto, 2018). Sin embargo, dado los precios actuales de arveja verde (alrededor de 250 U$S/tn), hacen mucho más interesante la posibilidad de incluirla en los sistemas de producción. Sumado al beneficio del margen de los planteos agrícolas, una vía de uso diferente a la exportación o al consumo humano directo, es su inclusión en las dietas forrajeras destinadas a alimentar bovinos de carne o de leche, como así también porcinos o aves. Abundan en la bibliografía internacional los trabajos donde se demuestra que el uso de arveja en reemplazo parcial de otras harinas proteicas, como la de soja y fuentes energéticas como el maíz, conducen a ganancias de peso similares o superiores a los testigos (Lardy et al, 2009; Fendrick et al, 2005; Soto Navarro et al, 2012; Pasinato et al, 2019; Landblom & Poland, 1997; Reed et al, 2004; Birkelo et al, 2000). Por todo esto, es importante conocer la adaptación de los diferentes materiales disponibles en el mercado a los ambientes productivos de Argentina.EEA PergaminoFil: Prieto, Gabriel María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros. Agencia de Extensión Rural Arroyo Seco; ArgentinaFil: Appella, Cristian Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Avila, F. CREA. Consorcio Regional de Experimentación Agrícola; ArgentinaFil: Bracco, V. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA). Sede Junín; ArgentinaFil: Brassesco, Raul Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná. Agencia de Extensión Rural Victoria; ArgentinaFil: Buschittari, D. Agricultores Federados Argentinos (AFA). Sociedad Cooperativa Limitada (SCL); ArgentinaFil: Casciani, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros. Agencia de Extensión Rural Arroyo Seco; ArgentinaFil: Espósito, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros; ArgentinaFil: Fariña, Leandro. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Agencia Regional de Desarrollo Productivo; ArgentinaFil: Fekete, Ana Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Frolla, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; ArgentinaFil: Gallego, Juan José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Valle Inferior de Río Negro; ArgentinaFil: Introna, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Agronomía; ArgentinaFil: Lavilla, M. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA). Sede Junín; ArgentinaFil: Maggio, J.C. Agrar del Sur; ArgentinaFil: Prece, Natalia María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Agronomía; ArgentinaFil: Maggio, María Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Mariotti Martinez, Jorge Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Martinez, S. Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA). Sede Junín; ArgentinaFil: Orliacq, A. Ministerio de Agroindustria de la Provincia de Buenos Aires. Chacra Experimental Pasman; ArgentinaFil: Vallejo, Maximiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná. Agencia de Extensión Rural Victoria; ArgentinaFil: Zgrablich, A. Universidad Nacional de Córdoba (UNC); Argentin