ОСНОВНЫЕ ИТОГИ НАУЧНЫХ ИССЛЕДОВАНИЙ В ОБЛАСТИ ВЕТЕРИНАРНОЙ ПАРАЗИТОЛОГИИ

The results of scientific researches in veterinary parasitology are given.Приведены результаты научных исследований в области ветеринарной паразитологии

ПРОГНОЗ ЭПИЗООТИЧЕСКОЙ СИТУАЦИИ В РОССИЙСКОЙ ФЕДЕРАЦИИ ПО ОСНОВНЫМ ГЕЛЬМИНТОЗАМ ЖИВОТНЫХ

The forecast of epizootic situation on the main helminthosis of animals in Russian Federation is provided.Приведен прогноз эпизоотической ситуации по основным гельминтозам животных в Российской Федерации

ОСТРАЯ ТОКСИЧНОСТЬ СУПРАМОЛЕКУЛЯРНЫХ КОМПЛЕКСОВ ФЕНБЕНДАЗОЛА, ПОЛУЧЕННЫХ ПО МЕХАНОХИМИЧЕСКОЙ ТЕХНОЛОГИИ С ИСПОЛЬЗОВАНИЕМ АДРЕСНОЙ ДОСТАВКИ DRUG DELIVERY SYSTEM

Objective of research: studying of acute toxicity of supramolecular complexes of Fenbendazole developed using chemical mechanical technology in comparison with the substance of preparation injected to the stomach of white mice and rats. Materials and methods: The research of parameters of acute toxicity of the supramolecular complexes of Fenbendazole was conducted according to «Guidelines for experimental (pre-clinical) studies of new pharmaceutical compounds», and other rules. Preparations were injected to the stomach of white rats and mice of both gender with the body mass 160–180 and 18–20 g. at a single dose 7 000, 10 000, 15 000 and 20 000 mg/ kg, respectively. Each dose was investigated on 6 white rats and 10 white mice. Within 14 days we observed the general health status and behavior of animals, symptoms of intoxication and eventual death. We also conducted postmortem examinations of the fallen animals. The parameters of acute toxicity were determined by the method of Litchfield J. and Wilcoxon F. (M.D. Belen’ky, 1963). Results and discussion: It was not possible to detect LD50 in supramolecular complex of Fenbendazole and the base  drug Fenbendazole due to the low toxicity. The maximum endurable dose of supramolecular complex no. 2 was 10 000 mg/kg, LD50 -value in supramolecular complex of Fenbendazole no. 2 at injection to the stomach of white mice and rats was 20 000 mg/kg.Цель исследования – изучение острой токсичности супрамолекулярных комплексов фенбендазола, полученных по механохимической технологии в сравнении с субстанцией препарата при введении в желудок белым мышам и крысам. Материалы и методы. Изучение параметров острой токсичности супрамолекулярных комплексов с фенбендазолом проводили согласно «Руководству по экспериментальному (доклиническому) изучению новых фармакологических веществ» и других правил. Препараты вводили подопытным белым крысам и мышам обоего пола массой тела 160–180 и 18–20 г соответственно однократно в желудок в дозах 7 000, 10 000, 15 000 и 20 000 мг/кг по препарату. Исследование каждой дозы проводили на 6 белых крысах и 10 белых мышах. В течение 14 сут наблюдали за общим состоянием и поведением животных, проявлением симптомов интоксикации и возможной гибелью. Проводили патологоанатомическое вскрытие павших животных. Показатели острой токсичности определяли по Литчфилду и Уилкоксону (М. Д. Беленький, 1963). Результаты и обсуждение. ЛД50 супрамолекулярного комплекса фенбендазола и базового препарата – фенбендазола установить не удалось из-за низкой токсичности. Максимально переносимая доза супрамолекулярного комплекса № 2 составила 10 000 мг/ кг, ЛД50 супрамолекулярного комплекса с фенбендазолом № 2 при введении в желудок белым мышам и крысам – 20 000 мг/кг

ЭФФЕКТИВНОСТЬ КОМБИНИРОВАННОГО ПРЕПАРАТА НА ОСНОВЕ НИКЛОЗАМИДА ПРОТИВ АНОПЛОЦЕФАЛЯТ РАЗНЫХ ВИДОВ И РАЗНОГО ВОЗРАСТА

Effect of Vigisox, Praziquantel, Fenbendazole, Albendazole and Niclozamide at the doses of 60, 3, 10, 10 and 100 mg/kg respectively applied against Moniezia spp. of different ages in sheep spontaneously infected with Moniezia expansa and M. benedeni is studied. Efficacy of drugs applied against mature Moniezia spp., was the following: Vigisox, Niclozamide and Praziquantel – 100 %, Albendazole – 88, Fenbendazole – 84 %, and against immature Moniezia spp.: Vigisox, Niclozamide – 89,3 %, Praziquantel – 100, Albendazole – 75 and Fenbendazole – 64,3 % respectively. The results shown a non significant difference in efficacy of Vigisox applied against various types of Anoplocephala spp. The drug at a dose of 60 mg/kg has shown a 97,9 % efficacy against M. expansa, 97,5 % – M. benedeni, 98,9 % – Thysaniezia giardi. While conducting preimaginal dehelminthizations of animals it is necessary to apply anthelmintics taking into account their effect on immature Moniezia spp. Изучено действие вигисокса, празиквантела, фенбендазола, альбендазола и никлозамида в дозах соответственно 60, 3, 10, 10 и 100 мг/кг против мониезий разного возраста на овцах, спонтанно инвазированных Moniezia expansa и M. benedeni. Эффективность против половозрелых Moniezia spp. составила вигисокса, никлозамида и празиквантела 100 %, альбендазола 88,0 и фенбендазола 84,0 %, а против неполовозрелых соответственно 89,3 %, 89,3, 100, 75 и 64,3 %. Получена незначительная разница в эффективности вигисокса против аноплоцефалят разных видов. Препарат в дозе 60 мг/кг проявил эффективность, равную против M. expansa 97,9 %, M. benedeni 97,5 и Thysaniezia giardi 98,9 %. Для проведения преимагинальных дегельминтизаций животных целесообразно применять антигельминтики с учетом их действия против неполовозрелых мониезий.

ЭФФЕКТИВНОСТЬ СУПРАМОЛЕКУЛЯРНОГО КОМПЛЕКСА ФЕНБЕНДАЗОЛА ПРИ НЕМАТОДОЗАХ ОВЕЦ

 Objective of research: to study the anthelmintic efficacy of the supramolecular complex of Fenbendazole used against different nematode species in sheep Materials and methods: Experiments were carried out on young sheep spontaneously infected with gastrointestinal strongylates (48 head), Dictyocaulus filaria (42 head), Strongyloides papillosus (21 head) и Trichocephalus ovis (24 head). In each helminthiasis, the supramolecular complex of Fenbendazole was given once orally to sheep from various groups at the dose of 3,0; 2,0 and 1,0 mg a.i./kg in comparison with the base preparation Fenbendazole at the doses of 1,0 and 3,0 mg/kg. Sheep which didn’t receive the drug served as controls. The efficacy of drugs was evaluated before and 18 days after dehelmintization according to the results of coprolarvoscopic examination by flotation and G. Baermann methods. The registration of drug activity was performed using the «control test». Results and discussion: Anthelmintic efficacy was studied and a therapeutic dose for the supramolecular complex of Fenbendazole produced by chemical mechanical technology using the Drug Delivery System was determined. In gastrointestinal strongylatoses the supramolecular complex against D. filaria. at the doses of 3,0; 2,0 and 1,0 mg a.i./kg showed the efficacy of 100; 93,4 and 78% , respectively. The efficacy of supramolecular complex at the dose of 3,0 mg/kg against S. papillosus was 100 %, and against T. ovis - 98,3 % at 10–13% efficacy of the base preparation Fenbendazole at the dose of 1,0 mg/kg. The therapeutic dose for the supramolecular complex at main nematodiasis in sheep was 3,0 mg a.i./kg. Цель исследования – изучение антигельминтной эффективности супрамолекулярного комплекса фенбендазола против разных видов нематод у овец. Материалы и методы. Опыты проводили на молодняке овец, спонтанно инвазированном желудочно-кишечными стронгилятами (48 гол.), Dictyocaulus filaria (42 гол.), Strongyloides papillosus (21 гол.) и Trichocephalus ovis (24 гол.). При каждом гельминтозе овцам разных групп задавали однократно перорально супрамолекулярный комплекс фенбендазола в дозе 3,0; 2,0 и 1,0 мг/кг по ДВ в сравнении с базовым препаратом фенбендазолом в дозах 1,0 и 3,0 мг/кг. Контролем служила группа овец, не получавшая препарат. Эффективность препаратов учитывали по результатам копрооволарвоскопических исследований методом флотации и Бермана до и через 18 сут после дегельминтизации. Учет активности препаратов проводили по типу «контрольный тест». Результаты и обсуждение. Изучена антигельминтная эффективность и установлена терапевтическая доза супрамолекулярного комплекса фенбендазола, полученного по механохимической технологии с адресной доставкой Drug Delivery System. Комплекс в дозах 3,0; 2,0 и 1,0 мг/кг по ДВ показал соответственно 100; 93,4 и 78%-ную эффективность при  стронгилятозах пищеварительного тракта, 100; 92,4 и 76,0%-ную – против D. filaria. Эффективность комплекса в дозе 3,0 мг/кг составила против S. papillosus 100 % и против T. ovis 98,3 % при 10–13%-ной эффективности базового препарата – фенбендазола в дозе 1,0 мг/кг. Терапевтическая доза супрамолекулярного комплекса при основных нематодозах овец составила 3,0 мг/кг по ДВ

Results of scientific researches in veterinary parasitology

The results of scientific researches in veterinary parasitology are given

The forecast of epizootic situation on the main helminthosis of animals in Russian Federation

The forecast of epizootic situation on the main helminthosis of animals in Russian Federation is provided

ACUTE TOXICITY OF SUPRAMOLECULAR ANTHELMINTIC COMPLEXES OF FENBENDAZOLE DEVELOPED BY CHEMICAL MECHANICAL TECHNOLOGY USING THE DRUG DELIVERY SYSTEM

Objective of research: studying of acute toxicity of supramolecular complexes of Fenbendazole developed using chemical mechanical technology in comparison with the substance of preparation injected to the stomach of white mice and rats. Materials and methods: The research of parameters of acute toxicity of the supramolecular complexes of Fenbendazole was conducted according to «Guidelines for experimental (pre-clinical) studies of new pharmaceutical compounds», and other rules. Preparations were injected to the stomach of white rats and mice of both gender with the body mass 160–180 and 18–20 g. at a single dose 7 000, 10 000, 15 000 and 20 000 mg/ kg, respectively. Each dose was investigated on 6 white rats and 10 white mice. Within 14 days we observed the general health status and behavior of animals, symptoms of intoxication and eventual death. We also conducted postmortem examinations of the fallen animals. The parameters of acute toxicity were determined by the method of Litchfield J. and Wilcoxon F. (M.D. Belen’ky, 1963). Results and discussion: It was not possible to detect LD50 in supramolecular complex of Fenbendazole and the base  drug Fenbendazole due to the low toxicity. The maximum endurable dose of supramolecular complex no. 2 was 10 000 mg/kg, LD50 -value in supramolecular complex of Fenbendazole no. 2 at injection to the stomach of white mice and rats was 20 000 mg/kg

EFFICACY OF COMBINED NICLOSAMIDE-BASED DRUG AGAINST ANOPLOCEPHALA SPP. OF DIFFERENT TYPES AND AGES

Effect of Vigisox, Praziquantel, Fenbendazole, Albendazole and Niclozamide at the doses of 60, 3, 10, 10 and 100 mg/kg respectively applied against Moniezia spp. of different ages in sheep spontaneously infected with Moniezia expansa and M. benedeni is studied. Efficacy of drugs applied against mature Moniezia spp., was the following: Vigisox, Niclozamide and Praziquantel – 100 %, Albendazole – 88, Fenbendazole – 84 %, and against immature Moniezia spp.: Vigisox, Niclozamide – 89,3 %, Praziquantel – 100, Albendazole – 75 and Fenbendazole – 64,3 % respectively. The results shown a non significant difference in efficacy of Vigisox applied against various types of Anoplocephala spp. The drug at a dose of 60 mg/kg has shown a 97,9 % efficacy against M. expansa, 97,5 % – M. benedeni, 98,9 % – Thysaniezia giardi. While conducting preimaginal dehelminthizations of animals it is necessary to apply anthelmintics taking into account their effect on immature Moniezia spp

EFFICACY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLE AGAINST NEMATODIASIS IN SHEEP

Objective of research: to study the anthelmintic efficacy of the supramolecular complex of Fenbendazole used against different nematode species in sheep Materials and methods: Experiments were carried out on young sheep spontaneously infected with gastrointestinal strongylates (48 head), Dictyocaulus filaria (42 head), Strongyloides papillosus (21 head) и Trichocephalus ovis (24 head). In each helminthiasis, the supramolecular complex of Fenbendazole was given once orally to sheep from various groups at the dose of 3,0; 2,0 and 1,0 mg a.i./kg in comparison with the base preparation Fenbendazole at the doses of 1,0 and 3,0 mg/kg. Sheep which didn’t receive the drug served as controls. The efficacy of drugs was evaluated before and 18 days after dehelmintization according to the results of coprolarvoscopic examination by flotation and G. Baermann methods. The registration of drug activity was performed using the «control test». Results and discussion: Anthelmintic efficacy was studied and a therapeutic dose for the supramolecular complex of Fenbendazole produced by chemical mechanical technology using the Drug Delivery System was determined. In gastrointestinal strongylatoses the supramolecular complex against D. filaria. at the doses of 3,0; 2,0 and 1,0 mg a.i./kg showed the efficacy of 100; 93,4 and 78% , respectively. The efficacy of supramolecular complex at the dose of 3,0 mg/kg against S. papillosus was 100 %, and against T. ovis - 98,3 % at 10–13% efficacy of the base preparation Fenbendazole at the dose of 1,0 mg/kg. The therapeutic dose for the supramolecular complex at main nematodiasis in sheep was 3,0 mg a.i./kg