シンナー遊びをしたのち泳いでいて死亡した1例

A 25-year-old male, a thinner abuser, swam in a pond just after thinner-sniffing and he drowned. Death from drowning was confirmed by diatoms in the lung and kidney. The drowning would seem to be induced by the effect of considerable high concentrations of toluene, absorbed into the body from thinner-sniffing, in tissues of the cadaver

分光光度法による一酸化炭素ヘモグロビン飽和度測定のための試料の選択

Carboxyhemoglobin (HbCO) saturation in a liquid specimen collected at medicolegal autopsy was analyzed firstly by a spectrophotometric method, which was first reported by Fretwurst et al, modified by Fukui and then modified by the authors, and secondly by a carbon monoxide-total hemoglobin (CO-Total Hb) method developed by the authors. In 70 blood specimens collected from the heart or blood vessels of 59 cadavers, the values obtained by the spectrophotometric method were similar to those obtained by the CO-Total Hb method with the exception of three blood specimens, two of which were markedly putrefied and the other considerably denatured by heat, in which the values obtained by the spectrophotometric method were significantly higher than those by the CO-Total Hb method. In 62 specimens of reddish discolored body cavity fluids collected from 31 cadavers, nearly all values obtained by the spectrophotometric method were much higher than those obtained by the CO-Total Hb method. The results indicate that the spectrophotometric method should not be used for the quantitative determination of HbCO in body cavity fluids, blood mixed with body cavity fluid, or blood markedly denatured by putrefaction or heat

パラコート中毒死の1例と生体試料中パラコートの迅速分析法

In a case of fatal paraquat poisoning, the distribution of paraquat was determined by a) the column chromatographic method developed by Tompsett, b) the ion-pairing method, c) the deproteinization method both developed by Jarvie et al, and d) the precipitation method developed by the authors. The precipitation method relies on the use of a precipitate of paraquat with Reinecke's salt to extract paraquat from biological material, and the dithionite color reaction to detect paraquat. The paraquat concentrations obtained by the precipitation method approximated to those obtained by the column chromatographic method. The results indicate that the use of the precipitation method followed by the dithionite color reaction is suitable not only for forensic practice but also emergency analysis. Since paraquat was not detected in the gastric contents collected at autopsy, and paraquat concentrations in the organs were much higher than the concentration in the heart blood, it seems that the hemodialysis and hemoperfusion were begun too late

淡水に溺没させたラットの血液及び体液中における一酸化炭素の死後産生

Rats were drowned in fresh water collected from a river, and kept immersed in the water for four weeks in separate bottles. When five rats were placed outside in the shade, where the temperature varied from 1 to 9°C, carboxyhemoglobin (HbCO) saturations in the blood and thoracic cavity fluid were not more than 1% and 10% respectively. When fifteen rats were immersed at 8-9°C, HbCO saturations in the body cavity fluids were more than 20 % in six. HbCO saturations in the blood, however, were not more than 10 % in all cases. The results indicate that low carbon monoxide (CO) levels are produced in the blood and high CO levels are formed in the body cavity fluid, and that body cavity fluid should not be used for CO determination.This study was supported by Grant-in-Aid for Scientific Research No. 58480205 from the Ministry of Education, Science and Culture, Japan