An in vitro Evaluation on a Novel Root Canal Cleansing Method by Using Nylon Fibers

Despite traditional metal-based dental files, such as NiTi being demonstrated effective in root cleaning, the tooth structure is always damaged. Thus, to fulfill the need for a minimally invasive tool for contemporary endodontics and dentistry, the use of polymer fibers might provide a good option, as it is soft, fabricable, and disposable. In this study, two types of nylon fibers with respective average diameters of 206.9 µm (fiber W) and 156.4 µm (fiber B), respectively, were used as dental files, and mounted onto either a reciprocating or a low-speed rotary hand-piece. In vitro, simulated root canal models were colored red using nail varnish, and then cleaned by the fiber files mounted on the hand-pieces. Three parts of the simulated models, i.e., the apical third, the medium third, and the coronal third, were chosen to assess the cleaning the efficiency (CE) of each specimen by calculating the ratio of the cross-sectional area changes, before and after cleansing, using micro-Computer Tomography (CT). A NiTi file with a low-speed hand-piece was used as a control. SEM was used to observe the nylon fiber surfaces before and after the cleansing. Micro-CT results showed that for both the nylon fibers, W and B, an average CE of 82.11% ± 9.68% for the medium third could be achieved, which is statistically higher (p < 0.01) than the coronal third and apical third. The cleansing efficiency was not affected by, the types of fibers, nor the hand-pieces according to student’s t-test. Most of the nylon fibers could withstand deformation after the cleansing. To conclude, nylon fiber files have demonstrated a certain cleansing efficiency in simulated root canals, and micro-CT is a promising method to assess CE.published_or_final_versio

Characterization of a new polysiloxane-based titanium surface treatment for resin titanium bonding

PURPOSE: To characterize the polysiloxane-coated titanium surface with thermal treatment by XPS and AFM. Such a novel coating might significantly enhance resin bonding. METHODS AND MATERIALS: A layer (2mm) of silicone grease (Dow Corning Co., Michigan, USA) was applied on two titanium plates (Permascand, Ljungaverk, Sweden) followed by a thermal treatment at two temperatures, 800 and 1100 ◦C, respectively. Another titanium plate was surface treated by sandblasting. The atomic composition of titanium surfaces and change of chemical state…postprin

Fluoride and carbonate co-incorporated porcine bone derived biological apatite stimulates osteogenesis in vitro via WNT/beta-catenin pathway

Poster Presentation - Biomaterials and cellular signalingBiological apatite (BAp) is widely recognized as a favorable substitute in bone tissue engineering due to it being biodegradable, biocompatible, and bearing osteoconductive properties. Incorporation of trace ions into BAp was reported to contribute to the physicochemical and biological properties of the substitute ...postprin

Resin-Zirconia bonding promotion in vitro with a zirconate coupling agent

Oral Session: Dental Materials 3: Ceramic-based Materials and Cements - 193. Keynote Address; Bond Strength to Ceramics and Dentin: no. 1553OBJECTIVE: Silane-aided bonding is widely acknowledged, but new coupling agents may be needed for enhanced bonding. The aim of this study was to evaluate in vitro the effect of a zirconate coupling agent application on the bonding between a flowable resin-composite and zirconia. It was hypothesized that the zirconate coupling agent would promote resin-composite adhesion better than using a commercial primer on a tribochemically silica-coated zirconia surface. METHOD: Various amounts (0.2, 0.4, 0.6, 0.8 and 1.0 vol%) of an organo-zirconate, a neoalkoxy zirconate coupling agent, Zr(IV)-2,2(bis-2-propenolatomethyl)- butanolato-tris-2-methyl-2-propenoato-O (NZ-33, Kenrich Petrochemical, USA) were diluted in absolute ethanol and applied ...postprin

Novel organofunctional silane coatings on silica-coated Ti

This in vitro study characterizes a developed novel silane system method to produce coatings on Ti. Hydrophobic coatings are needed in prosthetic dentistry to promote durable adhesion between luting resin cements and coated Ti surfaces. Silane blends may contribute to significantly higher bond strengths than functional silanes alone. We have previously shown that the novel silane system blend with 1.0 vol% of 3-acryloxypropyltrimethoxysilane + 0.3 vol% bis-1,2-(triethoxysilyl)ethane produced in vitro the highest shear bond strength after artificial aging. The silane primers were prepared of 3-acryloxypropyltrimethoxysilane and bis-1,2-(triethoxysilyl)ethane (in four different concentrations) and diluted in acidified ethanol-water solvent. The contact angles, surface free energies and critical surface tensions were assessed and the chemical compositions of treated surfaces were analyzed using X-photoelectron spectroscopy. A commercially available preactivated dental silane were used as the controls. Novel silane + silica coatings were successfully developed and optimized. We may conclude that: 1. the novel silane system was developed and optimized successfully; 2) The surface characterization showed nearly full coverage of silane coatings on titanium surface without compromising the surface roughness. 3) Such hydrophobic coatings may have huge potential for durable restoration bonding i dentistry.abstractNanotech Conference & Expo 2012, Santa Clara, CA., 18-21 June 2012. In Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy, 2012 (v. 3), p. 122-12

Effects of collaborative learning by using Facebook blended with PBL: a pilot study

SEAADE Scientific Session 1: Oral presentationConference Theme: Revolutionizing Educatio

Fatigue resistance of CAD/CAM crowns restored on premolars

The Congress was held in conjunction with 68th Meeing of the Japanese Society for Dental Materials and Devices, and 2016 Annual Meeting of the Indonesian Society for Dental Materials Science and TechnologyOral Presentation V: Clinical Application - paper no. O-2

Effects of cold-cured PMMA reinforcement by E-glass fibers impregnation

Poster Session: 87. Dental Materials: Prosthodontics-Provisional/Impression/Materials: no. 679OBJECTIVES: Cold-cured PMMA is a common material for fabrication of appliances such as orthodontic retainers. However, cold-cured PMMA has lower degree of polymerization than the heat-cured and this leads to inferior biomechanical properties and fracture vulnerability. Reinforcement of cold-cured PMMA using chopped and impregnated E-glass fibers was hypothesized to be a solution. METHODS: Specimens of cold-cured PMMA powder mixed with different concentrations of chopped E-glass fibers (0 - 5 wt.%) were prepared by using a new mixing method which does not need addition of extra monomer (MMA). After the specimens were stored at various conditions (room temperature and ambient humidity for 1 day and 7 days; water storage at 37oC for 7, 14 and 30 days), 3-point bending tests were performed to test the flexural strength and SEM images were taken to assess the fractured morphology and fibers embedment to the PMMA matrix. The data was analyzed by 1-way ANOVA and Tukey post hoc test. RESULTS: In general, all fiber-impregnated cold-cured PMMA showed a higher flexural strength than the control at various storage conditions, except for 1 wt.% fiber concentration. The reinforcing ability decreased with increase in the duration of water storage condition. SEM images showed a good impregnation of E-glass fibers to PMMA matrix. CONCLUSIONS: A new E-glass fiber impregnation method in cold-cured PMMA, which is immediately transferable and usable in dental clinics/laboratories, was successfully developed. Such method may increase of the cold-cured PMMA flexural strength.link_to_OA_fulltex

In vitro evaluation of novel electrospun oxide-scaffold biocomposites

OBJECTIVE: The study of this in vitro study was to evaluate the biological performance of several novel oxides-loaded poly(lactic-L-glycolic acid) (PLLA) scaffold biocomposites, which were fabricated by electrospinning method, to demonstrate their use in bone tissue engineering. METHOD: Silicon dioxide (SiO2), Zirconium dioxide (ZrO2), Titanium dioxide (TiO2) and Hafnium dioxide (HfO2) conjugated with PLLA were developed and fabricated by electrospinning the biocomposite scaffolds, namely PLLA/SiO2, PLLA/ZrO2, PLLA/TiO2, and PLLA/HfO2. These biocomposite were characterized by SEM and EDX analyses. In vitro studies such as biomineralization, protein absorption, cytotoxicity were also carried out. RESULT: The scaffold possessed a porous nature with pore dimensions suitable for cell infiltration and colonization. The presence of zirconia and hafnia in the PLLA/ZrO2 and PLLA/HfO2 scaffold increased protein adsorption and biomineralization properties. The PLLA/ZrO2 and PLLA/HfO2 scaffold were also found to be non-toxic to osteoblast MC3T3-E1 cells. CONCLUSION: The novel electrospun PLLA/oxide scaffold biocomposites harbor the desired features such as biocompatibility and porous structure to serve as template for bone tissue engineering. In particular, PLLA/ZrO2 and PLLA/HfO2 are the highest potential candidates to be used for bone tissue engineering