Flow cytometric measurement of glutathione content of human cancer biopsies.

Rice et al. (1986) have described a flow cytometric method where the non-fluorescent probe monochlorobimane (mBCl) forms a fluorescent adduct with cellular glutathione (GSH) under the action of glutathione-S-transferase. We show here that for EMT6 carcinosarcoma cells there is a close correlation between mean cell fluorescence, expressed as a ratio to that of fluorescence calibration beads, and biochemically determined GSH content over the range 0.2-2.0 fmol cell-1. Single cell suspensions from 14 human cancers were prepared by 23-gauge needle aspiration or mechanical disaggregation of surgical specimens, stained using mBCl and examined by flow cytometry. There was a wide range in individual cell fluorescence, which in contrast to EMT6 cells was not strongly correlated with Coulter volume. By comparing tumour cell fluorescence to that of calibration beads, and assuming that the relationship with GSH content for EMT6 holds for other cells, a mean GSH content of 0.95 fmol cell-1 was derived for nine carcinomas, and 0.21 fmol cell-1 for five non-Hodgkin's lymphomas. Although this semi-quantitation needs further validation, the method used here is rapid, gives an indication of heterogeneity of tumour cell GSH content, and can be applied to fine needle biopsy samples. It therefore shows promise as a means for studying prospectively the relationship of GSH content to clinical drug and radiation sensitivity, and for monitoring the effects of agents such as buthionine sulphoximine which are intended to improve treatment results through tumour cell GSH depletion

The Deuterium, Oxygen, and Nitrogen Abundance Toward LSE 44

We present measurements of the column densities of interstellar DI, OI, NI, and H2 made with FUSE, and of HI made with IUE toward the sdO star LSE 44, at a distance of 554+/-66 pc. This target is among the seven most distant Galactic sight lines for which these abundance ratios have been measured. The column densities were estimated by profile fitting and curve of growth analyses. We find D/H = (2.24 +1.39 -1.32)E-5, D/O = (1.99 +1.30 -0.67)E-2, D/N = (2.75 +1.19 -0.89)E-1, and O/H = (1.13 +0.96 -0.71)E-3 (2 sigma). Of the most distant Galactic sight lines for which the deuterium abundance has been measured LSE 44 is one of the few with D/H higher than the Local Bubble value, but D/O toward all these targets is below the Local Bubble value and more uniform than the D/H distribution. (Abstract abridged.)Comment: 20 pages, including 9 figures. Accepted for publication in Ap

A Near-Solar Metallicity, Nitrogen-Deficient Lyman Limit Absorber Associated with two S0 Galaxies

From UV spectra of the bright quasar PHL 1811 recorded by FUSE and the E140M configuration on STIS, we have determined the abundances of various atomic species in a Lyman limit system at z = 0.0809 with log N(H I) = 17.98. Considerably more hydrogen may be in ionized form, since the abundances of C II, Si II, S II and Fe II are very large compared to that of O I, when compared to their respective solar abundance ratios. Our determination [O/H] = -0.19 in the H I-bearing gas indicates that the chemical enrichment of the gas is unusually high for an extragalactic QSO absorption system. However, this same material has an unusually low abundance of nitrogen, [N/O] < -0.59, indicating that there may not have been enough time during this enrichment for secondary nitrogen to arise from low and intermediate mass stars. In an earlier investigation we found two galaxies at nearly the same redshift as this absorption system and displaced by 34 and 87 kpc from the line of sight. An r-band image recorded by the ACS on HST indicates these are S0 galaxies. One or both of these galaxies may be the source of the gas, which might have been expelled in a fast wind, by tidal stripping, or by ram-pressure stripping. Subtraction of the ACS point-spread function from the image of the QSO reveals the presence of a face-on spiral galaxy under the glare of the quasar; although it is possible that this galaxy may be responsible for the Lyman limit absorption, the exact alignment of the QSO with the center of the galaxy suggests that the spiral is the quasar host.Comment: 74 pages, 14 figures; to be published in the Astrophysical Journal (Part 1) May 1, 2005 issue. A version of the paper with figures of better quality may be found at http://www.astro.princeton.edu/~ebj/PHL1811_paper.ps (postscript) or http://www.astro.princeton.edu/~ebj/PHL1811_paper.pdf (pdf

Physical Properties of Complex C Halo Clouds

Observations from the Galactic Arecibo L-Band Feed Array HI (GALFA-HI) Survey of the tail of Complex C are presented and the halo clouds associated with this complex cataloged. The properties of the Complex C clouds are compared to clouds cataloged at the tail of the Magellanic Stream to provide insight into the origin and destruction mechanism of Complex C. Magellanic Stream and Complex C clouds show similarities in their mass distributions (slope = -0.7 and -0.6, respectively) and have a common linewidth of 20 - 30 km/s (indicative of a warm component), which may indicate a common origin and/or physical process breaking down the clouds. The clouds cataloged at the tail of Complex C extend over a mass range of 10^1.1 to 10^4.8 solar masses, sizes of 10^1.2 to 10^2.6 pc, and have a median volume density of 0.065 cm^(-3) and median pressure of (P/k) = 580 K cm^{-3}. We do not see a prominent two-phase structure in Complex C, possibly due to its low metallicity and inefficient cooling compared to other halo clouds. From assuming the Complex C clouds are in pressure equilibrium with a hot halo medium, we find a median halo density of 5.8 x 10^(-4) cm^(-3), which given a constant distance of 10 kpc, is at a z-height of ~3 kpc. Using the same argument for the Stream results in a median halo density of 8.4 x 10^(-5) x (60kpc/d) cm^(-3). These densities are consistent with previous observational constraints and cosmological simulations. We also assess the derived cloud and halo properties with three dimensional grid simulations of halo HI clouds and find the temperature is generally consistent within a factor of 1.5 and the volume densities, pressures and halo densities are consistent within a factor of 3.Comment: Accepted for publication in AJ. 54 pages, including 6 tables and 16 figure

Spatial Variability in the Ratio of Interstellar Atomic Deuterium to Hydrogen. I. Observations toward delta Orionis by the Interstellar Medium Absorption Profile Spectrograph

Studies of the abundances of deuterium in different astrophysical sites are of fundamental importance to answering the question about how much deuterium was produced during big bang nucleosynthesis and what fraction of it was destroyed later. With this in mind, we used the Interstellar Medium Absorption Profile Spectrograph (IMAPS) on the ORFEUS-SPAS II mission to observe at a wavelength resolution of 4 km/s (FWHM) the L-delta and L-epsilon absorption features produced by interstellar atomic deuterium in the spectrum of delta Ori A. A chi-square analysis indicated that 0.96 < N(D I)< 1.45e15 cm^{-2} at a 90% level of confidence, and the gas is at a temperature of about 6000K. To obtain an accurate value of N(H I) needed for a determination of the atomic ratio of D to H, we measured the L-alpha absorption features in 57 spectra of delta Ori in the IUE archive. From our measurement of N(H I)= 1.56e20 cm^{-2}, we found that N(D I)/N(H I)= 7.4(+1.9,-1.3)e-6 (90% confidence). Our result for D/H contrasts with the more general finding along other lines of sight that D/H is approximately 1.5e-5. The underabundance of D toward delta Ori A is not accompanied by an overabundance of N or O relative to H, as one might expect if the gas were subjected to more stellar processing than usual.Comment: 37 pages, 6 figures. Submitted to the Astrophysical Journa

The Heavy Element Enrichment of Lyman alpha Clouds in the Virgo Supercluster

Using high S/N STIS echelle spectra (FWHM=7 km/s) of 3C 273, we constrain the metallicities of two Lya clouds in the vicinity of the Virgo cluster. We detect C II, Si II, and Si III absorption lines in the Lya absorber at z = 0.00530. Previous observations with FUSE have revealed Ly beta - Ly theta lines at this redshift, thereby accurately constraining N(H I). We model the ionization of the gas and derive [C/H] = -1.2^{+0.3}_{-0.2}, [Si/C] = 0.2+/-0.1, and log n_{H} = -2.8+/-0.3. The model implies a small absorber thickness, ~70 pc, and thermal pressure p/k ~ 40 cm^{-3} K. It is most likely that the absorber is pressure confined by an external medium because gravitational confinement would require a very high ratio of dark matter to baryonic matter. Based on Milky Way sight lines in which carbon and silicon abundances have been reliably measured in the same interstellar cloud (including new measurements presented herein), we argue that the overabundance of Si relative to C is not due to dust depletion. Instead, this probably indicates that the gas has been predominately enriched by Type II supernovae. Such enrichment is most plausibly provided by an unbound galactic wind, given the absence of galaxies within a projected distance of 100 kpc and the presence of galaxies capable of driving a wind at larger distances. We also constrain the metallicity and physical conditions of the Virgo absorber at z = 0.00337 based on detections of O VI and H I and an upper limit on C IV. If this absorber is collisionally ionized, the O VI/C IV limit requires T > 10^{5.3} K. For either collisional ionization or photoionization, we find that [O/H] > -2.0 at z = 0.00337.Comment: Final Ap.J. versio

OVI Absorption in the Milky Way Disk, and Future Prospects for Studying Absorption at the Galaxy-IGM Interface

We present a brief summary of results from our FUSE program designed to study OVI absorption in the disk of the Milky Way. As a full analysis of our data has now been published, we focus on the improvements that FUSE afforded us compared to Copernicus data published thirty years ago. We discuss FUSE's limitations in studying OVI absorption from nearby galaxies using background QSOs, but present FUSE spectra of two probes which indicate the absence of OVI (but the presence of Lyman-beta) absorption 8 and 63 kpc from a foreground galaxy. Finally, we discuss the need for a more sensitive UV spectrograph to map out the physical conditions of baryons around galaxies.Comment: Invited review to appear in the proceedings of "Future Directions in Ultraviolet Spectroscopy" meeting, held Oct 20-22, 2008, Annapolis, MD. To appear as an AIP Conference Proceedin

Agricultural Land Use Planning and Groundwater Quality

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75123/1/j.1468-2257.1983.tb00394.x.pd

Studying the Warm-Hot Intergalactic Medium in Emission

We assess the possibility to detect the warm-hot intergalactic medium (WHIM) in emission and to characterize its physical conditions and spatial distribution through spatially resolved X-ray spectroscopy, in the framework of the recently proposed DIOS, EDGE, Xenia, and ORIGIN missions, all of which are equipped with microcalorimeter-based detectors. For this purpose we analyze a large set of mock emission spectra, extracted from a cosmological hydrodynamical simulation. These mock X-ray spectra are searched for emission features showing both the OVII K alpha triplet and OVIII Ly alpha line, which constitute a typical signature of the warm hot gas. Our analysis shows that 1 Ms long exposures and energy resolution of 2.5 eV will allow us to detect about 400 such features per deg^2 with a significance >5 sigma and reveals that these emission systems are typically associated with density ~100 above the mean. The temperature can be estimated from the line ratio with a precision of ~20%. The combined effect of contamination from other lines, variation in the level of the continuum, and degradation of the energy resolution reduces these estimates. Yet, with an energy resolution of 7 eV and all these effects taken into account, one still expects about 160 detections per deg^2. These line systems are sufficient to trace the spatial distribution of the line-emitting gas, which constitute an additional information, independent from line statistics, to constrain the poorly known cosmic chemical enrichment history and the stellar feedback processes.Comment: 19 pages, 10 figures, ApJ in press; revised version according to revie

nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2-fixing community dynamics

A dataset of 87 020 nifH reads and 16 782 unique nifH protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N2-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of Alphaproteobacteria, Betaproteobacteria and Deltaproteobacteria, mainly relatives to Bradyrhizobium and Methylosinus/Methylocystis, but a surprising paucity of Gammaproteobacteria. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on nifH diversity. nifH gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N2-fixing bacteriaFil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Tripp, H. James. University of California. Department of Ocean Sciences; Estados UnidosFil: Frank, Ildiko E.. University of California. Department of Ocean Sciences; Estados UnidosFil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Calderoli, Priscila Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Donato, Mariano Humberto. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Sistemática y Biología Evolutiva; ArgentinaFil: Zehr, Jonathan P.. University of California. Department of Ocean Sciences; Estados UnidosFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Biotecnología y Biología Molecular; Argentin