Amarų pernešamo moliūgų geltonojo viruso (CABYV) molekulinis apibūdinimas Turkijoje

International audienceCucurbit aphid-borne yellows virus (CABYV) causes yellowing symptoms on the older leaves of plants from several species in the Cucurbitaceae family. Previous phylogenetic analyses showed the existence of two major clades of CABYV isolates related to their geographic origin – Asian vs European-African (Mediterranean) groups. To provide an understanding of the molecular variability of CABYV in Turkey, surveys were performed in 2011 to 2015 to obtain plants infected by the virus in four provinces, where cucurbit cultivation is of economic importance for the country. One hundred and forty leaf samples of cucurbit plants showing virus-like symptoms: yellowing, mosaic, deformation on leaves and stunting, were collected from Antalya, Ankara and Burdur provinces during the 2011–2013 growing seasons and 40 leaf samples from Tokat province during the 2015 growing season. Five CABYV isolates, collected in Antalya, Hatay and Mugla provinces in 1994, were added to the analysis. The coat protein coding regions of 15 CABYV isolates were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using specific primers and sequenced. According to the sequence analyses, 11 isolates shared 99% nucleotide identity with isolates from Egypt, Slovenia and Montenegro, three isolates showed more than 99% nucleotide identity with isolates from China, South Korea and Iran, and one isolate presented evidence of mixed infection. Neighbour-joining analyses based on the resultant sequences revealed that the Turkish CABYV isolates clustered in two distinct molecular groups: five isolates from 1994, six isolates from 2012–2013 and one from 2015 clustered in the Mediterranean group, whereas three isolates from 2012–2013 clustered in the Asian group. In addition, one isolate presented a mixed infection of the two groups

Molecular characterization of cucurbit aphid-borne yellows virus affecting cucurbits in Turkey

National audienceCucurbits are economically important vegetable in Turkey. Cucurbit crops are seriously affected by many viruses including Cucurbit aphid-borne yellows virus (CABYV). CABYV, a widespread polerovirus causing yellowing symptoms on older leaves that were previously associated to nutritional or physiological disorders. CABYV was first identified in 1988 in France, now, CABYV is widely spread in the whole Mediterranean region (Lecoq and Desbiez 2012). In Turkey, CABY reported firstly by Yardımcı and Özgönen (2007) as serologicaly. The purpose of this study was to characterize the molecular variability of CABYV in Turkey, through determining partial coat protein (CP) nucleotide sequences of (CABYV) isolates of Turkey

Presence of cucurbit viruses in Ankara and Antalya province and molecular characterization of coat protein gene of zucchini yellow mosaic virus turkish isolates

Cucurbits are widely grown vegetables in different provinces of Turkey. However their production is restricted by pests and pathogens including different plant viruses, among which Zucchini yellow mosaic virus (ZYMV) is particularly damaging. Infections by this virus during the early stages of plant development causes deformation of fruits and significant yield losses. During 2009-2014, 221 cucurbits samples were collected from fields and greenhouses from different provinces of Turkey. Serological testing was performed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for presence of Watermelon mosaic virus (WMV), ZYMV, Cucumber mosaic virus (CMV), Papaya ring spot virus (PRSV), cucumber green mottle mosaic virus (CGMMV) and Squash mosaic virus (SqMV). For molecular analaysis of ZYMV, full-length CP nucleotide sequences were obtained for 45 ZYMV isolates and compared with sequences from worldwide isolates available in databases. The CP coding regions of Turkish ZYMV were 837 nt long and encoded 279 amino acids (aa). Forty two isolates from different regions were classified in the molecular subgroup Al, the most frequent in the Mediterranean Basin, Europe and Africa. Three isolates collected from Antalya region were classified in subgroup A4 and showed 99 % sequence identity with isolates from the same subgroup that have emerged recently in France

Presence of cucurbit viruses in Ankara and Antalya province and molecular characterization of coat protein gene of zucchini yellow mosaic virus turkish isolates

Cucurbits are widely grown vegetables in different provinces of Turkey. However their production is restricted by pests and pathogens including different plant viruses, among which Zucchini yellow mosaic virus (ZYMV) is particularly damaging. Infections by this virus during the early stages of plant development causes deformation of fruits and significant yield losses. During 2009-2014, 221 cucurbits samples were collected from fields and greenhouses from different provinces of Turkey. Serological testing was performed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for presence of Watermelon mosaic virus (WMV), ZYMV, Cucumber mosaic virus (CMV), Papaya ring spot virus (PRSV), cucumber green mottle mosaic virus (CGMMV) and Squash mosaic virus (SqMV). For molecular analaysis of ZYMV, full-length CP nucleotide sequences were obtained for 45 ZYMV isolates and compared with sequences from worldwide isolates available in databases. The CP coding regions of Turkish ZYMV were 837 nt long and encoded 279 amino acids (aa). Forty two isolates from different regions were classified in the molecular subgroup Al, the most frequent in the Mediterranean Basin, Europe and Africa. Three isolates collected from Antalya region were classified in subgroup A4 and showed 99 % sequence identity with isolates from the same subgroup that have emerged recently in France

Presence of cucurbit viruses in Ankara and Antalya province and molecular characterization of coat protein gene of zucchini yellow mosaic virus turkish isolates

Cucurbits are widely grown vegetables in different provinces of Turkey. However their production is restricted by pests and pathogens including different plant viruses, among which Zucchini yellow mosaic virus (ZYMV) is particularly damaging. Infections by this virus during the early stages of plant development causes deformation of fruits and significant yield losses. During 2009-2014, 221 cucurbits samples were collected from fields and greenhouses from different provinces of Turkey. Serological testing was performed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for presence of Watermelon mosaic virus (WMV), ZYMV, Cucumber mosaic virus (CMV), Papaya ring spot virus (PRSV), cucumber green mottle mosaic virus (CGMMV) and Squash mosaic virus (SqMV). For molecular analaysis of ZYMV, full-length CP nucleotide sequences were obtained for 45 ZYMV isolates and compared with sequences from worldwide isolates available in databases. The CP coding regions of Turkish ZYMV were 837 nt long and encoded 279 amino acids (aa). Forty two isolates from different regions were classified in the molecular subgroup Al, the most frequent in the Mediterranean Basin, Europe and Africa. Three isolates collected from Antalya region were classified in subgroup A4 and showed 99 % sequence identity with isolates from the same subgroup that have emerged recently in France