In Vivo Application of Electric Fields Collaborate With rRHEB to Direct Optic Nerve Regeneration

Regenerating the optic nerve requires signals that both "fuel" and "steer" retinal ganglion cell (RGC) axon growth to appropriate targets. Although strategies to increase mammalian target of rapamycin (mTOR) activity have been employed to "fuel" RGC axon regeneration, they are not as effective at "steering" regeneration. Based on compelling evidence that electric field (EF) application does both, we investigated whether EFs-in conjunction with mTOR activation-direct more target-specific RGC axon regeneration than either treatment alone