Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats

OBJECTIVE: The aim of this study was to compare subgingival irrigation with tetracycline hydrochloride (TTC-HCL) as adjunctive treatment to scaling and root planning (SRP) on induced periodontitis in rats. MATERIAL AND METHODS: In 60 rats, periodontal disease was ligature-induced at the mandibular left first molar. After 7 days, the ligature was removed and all animals were submitted to SRP, and divided into 2 groups according to the following treatment: C (n=30) - subgingival irrigation with 1 mL of saline; T (n=30) - subgingival irrigation with 1 mL of TTC-HCL (50 mg/mL). Ten animals in each group were euthanized at 7, 15 and 30 days posttreatment. The histometric values were statistically analyzed (

Students’ Perspectives on Use Behaviour of Learning Management Systems in Sri Lankan Universities

This research aims to explore the perceptions of students in using Learning Management System (LMS) in order to enhance the learning process of undergraduates in government universities in Sri Lanka.Many universities in Sri Lanka have implemented LMS to make their learning interactive and engaging.These institutions have made considerable amount of investments in terms of finance and other resources but the benefits enjoyed are far below expectations.Hence, it is of high recognition that a study is very much needed to understand the reason(s) for such low usage in Sri Lankan free education context. Quantitative study based on self-administered questionnaire survey was conducted.Out of the 15 government universities in Sri Lanka, 03 were selected. Results from 344 valid responses reveals that Attitude, Experience and Anxiety significantly influence the students’ use behaviour of LMS in Sri Lankan universities, while Efficacy and Collaboration do not have any impact. The research identifies what factors would influence these students’ use behaviour of LMS in state universities. Variables Students’ Attitude towards LMS, Efficacy, Experience, Anxiety and Collaboration with Lecturers and Students are considered to be the predictor variables and their influence and impact on Use Behaviour of LMS (predicted variable) is studied

Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats

OBJECTIVE: The aim of this study was to compare subgingival irrigation with tetracycline hydrochloride (TTC-HCL) as adjunctive treatment to scaling and root planning (SRP) on induced periodontitis in rats. MATERIAL AND METHODS: In 60 rats, periodontal disease was ligature-induced at the mandibular left first molar. After 7 days, the ligature was removed and all animals were submitted to SRP, and divided into 2 groups according to the following treatment: C (n=30) - subgingival irrigation with 1 mL of saline; T (n=30) - subgingival irrigation with 1 mL of TTC-HCL (50 mg/mL). Ten animals in each group were euthanized at 7, 15 and 30 days posttreatment. The histometric values were statistically analyzed (p<0.05). RESULTS: In the histometric analysis, at 7, 15 and 30 days, Group T (0.72±0.05 mm(2), 0.57±0.14 mm(2), 0.62±0.07 mm(2)), showed less bone loss (p<0.05) than Group C (1.35±0.25 mm(2); 1.40±0.31 mm(2); 1.29±0.27 mm(2)), respectively. CONCLUSIONS: Subgingival irrigation with TTC-HCL was an effective adjunctive treatment for periodontal disease induced in rats

The ordinary becomes extraordinary: the occupation of living whilst dying

© 2012 Dr. Deidre Diane MorganPalliative care and occupational therapy research has rarely focused on the experience of disrupted embodiment and impact on everyday life. The purpose of this study was to better understand this lived experience for people with advanced cancer living at home, in Victoria, Australia. Employing a pragmatic qualitative approach informed by hermeneutic phenomenology as defined by van Manen (1990), it examined peoples’ lived experience of disrupted embodiment and the impact of this on everyday life. While initial analysis was guided by the structure of Colaizzi’s (1978) descriptive methods, final analysis utilised an interpretive approach. Consistent with an interpretive approach, conceptual frameworks of embodiment (Gadow, 1982; Leder, 1990) and occupation (Kielhofner, 2008) were used to direct inquiry and to understand better participants’ ways of being-in-the-world (Lopez & Willis, 2004; Toombs, 1995; van Manen, 1990; Wojnar & Swanson, 2007). The literature review highlights the extensive focus of palliative care on ameliorating physical and psycho-spiritual suffering. Maintaining active participation in everyday activities or occupations at the end-of-life receives minimal attention, yet available studies suggest participation facilitates adaptation between self and a rapidly changing body, and serves to ameliorate suffering related to functional decline. Despite functional decline, ongoing participation in day-to-day activities is highly valued by people at the end-of-life. In this study, people told their stories about living with rapidly disintegrating bodies and how this influenced their ability to participate in essential and valued occupations. Thematic analyses aimed to identify themes built from empirical data and evaluate these against the conceptual frameworks. Relentless bodily disintegration disrupts the person’s relationship with time and hinders ability to engage in everyday activities including self care and valued social and recreational pursuits. While people experience an ever shifting sense of self, and ability to engage in daily occupations is increasingly restricted, a desire to live as fully as possible only intensifies in the face of these changes. Contending with this deterioration whilst seeking to live as actively as possible is the work of adaptation at the end-of-life. As people strove to continue active engagement in everyday activities and developed spontaneous strategies to do so, for the most part this was without clinical guidance or support. Although several people expressed a desire for increased support to interpret changing bodies and assistance to optimise function wherever possible, this type of support was limited. Conventional rehabilitation models are not geared towards a downward functional trajectory and an alternative model of care is needed. The concept of habilitation (Koenig Coste, 2004; Raia, 1992) that focuses on optimising function rather than emphasising restoration, irrespective of direction or place in the disease trajectory, warrants further exploration. This thesis concludes by highlighting the need for clinician awareness of the ineffable nature of disrupted embodiment, and the way that routine nature of everyday occupation conceals the significance of participation at the end-of-life. A challenge is made for clinicians to look beyond effective physical and psycho-spiritual palliation as end points in themselves, to ways in which they can enable active participation in everyday occupations

Bone formed after maxillary sinus floor augmentation by bone autografting with hydroxyapatite and low-level laser therapy: A randomized controlled trial with histomorphometrical and immunohistochemical analyses

Purpose: The aim of this study was to evaluate the bone formed after maxillary sinus floor augmentation (MSFA) by bone autografting combined with hydroxyapatite (HA) that had been either treated with low-level laser therapy (LLLT) or not. Materials and Methods: Twelve biopsies were obtained from patients 6 months after MSFA using a combination of 50% of autogenous bone (AB) and 50% of HA (AB/HA group, n = 6) followed by LLLT (AB/HA-LLLT group, n = 6). The laser used in this study was gallium-aluminium-arsenide laser with a wavelength of 830 nm (40 mW; 5.32 J/point; 0.57 W/cm 2 ). Samples obtained were subjected to histological, histometric, and immunohistochemical analysis for detection of tartrate-resistant acid phosphatase and runt-related transcription factor 2. The data were submitted to statistical analysis (Shapiro-Wilk and Student t tests; α = 5%). Results: Statistical analysis revealed no significant difference in vital bone presence and immunohistochemical analysis between the groups. There was no reduction in bone marrow or fibrous tissue in the AB/HA group and AB/HA-LLLT group. There was a decrease in the amount of remaining biomaterial between the groups (P = 0.0081). Conclusion: LLLT did not increase the formation of new bone; instead, it accelerated the bone remodeling process

Comparative analysis of root surface smear layer removal by different etching modalities or erbium:yttrium-aluminum-garnet laser irradiation. A scanning electron microscopy study

The purpose of this study was to evaluate the effect of erbium:yttrium-aluminum-garnet (Er:YAG) laser (2.94 mu m) irradiation on the removal of root surface smear layer of extracted human teeth and to compare its efficacy with that of citric acid, ethylenediamine tetra-acetic acid (EDTA), or a gel containing a mixture of tetracycline hydrochloride (HCl) and citric acid, using scanning electron microscopy (SEM). Thirty human dentin specimens were randomly divided into six groups: G1 (control group), irrigated with 10 ml of physiologic saline solution; G2, conditioned with 24% citric acid gel; G3, conditioned with 24% EDTA gel; G4, conditioned with a 50% citric acid and tetracycline gel; G5, irradiated with Er:YAG laser (47 mJ/10 Hz/5.8 J/cm(2)/pulse); G6, irradiated with Er:YAG laser (83 mJ/10 Hz/10.3 J/cm(2)/pulse). Electron micrographs were obtained and analyzed according to a rating system. Statistical analysis was conducted with Kruskal-Wallis and Mann-Whitney tests (P < 0.05). G1 was statistically different from all the other groups; no statistically significant differences were observed between the Er:YAG laser groups and those undergoing the other treatment modalities. When the two Er:YAG laser groups were compared, the fluency of G6 was statistically more effective in smear layer removal than the one used in G5 (Mann-Whitney test, P < 0.01). Root surfaces irradiated by Er:YAG laser had more irregular contours than those treated by chemical agents. It can be concluded that all treatment modalities were effective in smear layer removal. The results of our study suggest that the Er:YAG laser can be safely used to condition diseased root surfaces effectively. Furthermore, the effect of Er:YAG laser irradiation on root surfaces should be evaluated in vivo so that its potential to enhance the healing of periodontal tissues can be assessed

Effectiveness of the diode laser in the treatment of ligature-induced periodontitis in rats: a histopathological, histometric, and immunohistochemical study

The aim of this study was to evaluate the effects of the use of a high-power gallium-aluminum-arsenide diode laser (GaAlAs; 808 nm, 1 W, 20 s, 20 Hz, 10 J) alone or as adjunctive therapy to scaling and root planing in the treatment of induced periodontitis in rats. Periodontitis was induced by placing a ligature around the mandibular first molar of 60 rats. After 7 days, the ligature was removed and the animals were divided into four groups as follows: C (control), no periodontal treatment; SRP, scaling and root planing (SRP); DL, diode laser (DL) irradiation treatment; and SRP/DL, both SRP and DL irradiation treatment. Five animals from each group were euthanized at 7, 15, and 30 days posttreatment. The effectiveness of the treatments was evaluated in the furcation area using histopathological analysis, histometric analysis of alveolar bone loss (ABL), and immunohistochemical detection of tartrate-resistant acid phosphatase (TRAP), runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN). DL, alone or in combination with adjunctive therapy to SRP in the treatment of experimental periodontitis, resulted in a decreased local inflammatory response. At 7-days posttreatment, the DL and SRP/DL groups had fewer TRAP-positive cells and more RUNX2-positive cells. There was greater OCN immunolabeling in the DL group than in the C and SRP groups at 15 days. There was less ABL in the DL and SRP/DL groups at 15 and 30 days. In conclusion, DL was effective in the treatment of ligature-induced periodontitis in rats, both when used alone and when used as adjunctive therapy to SRP

One-Stage Approach to Rehabilitate a Hopeless Tooth in the Maxilla by Means of Immediate Dentoalveolar Restoration: Surgical and Prosthetic Considerations

Contemporary dentistry has increased the demand for predictable functional and esthetic results in a short period of time without compromising the long-term success of rehabilitation. Recent advances in surgical techniques have provided alternatives that allow the prosthetic rehabilitation of complex implant-supported cases through minimally invasive techniques. In this context, immediate dentoalveolar restoration (IDR) was described aiming at restoring function and esthetics through the reconstruction of lost periodontal tissues followed by immediate implant placement in order to minimize treatment time and surgical morbidity in a one-stage approach. Therefore, the aim of this clinical case is to describe the reconstruction and rehabilitation of a hopeless tooth in the maxillary region in a one-stage approach by means of IDR. The proposed steps to rehabilitate the case involved atraumatic dental extraction, immediate implant placement, and hard tissue augmentation by means of cortical-medullary bone graft harvested from the maxillary tuberosity. Afterwards, a provisional restoration was manufactured and installed to the implant allowing immediate prosthesis provisionalization and function in the same operatory time. Six months after the surgical procedure, the final prosthesis was manufactured and installed. The follow-up of nine years demonstrated the preservation of hard and soft tissue without tissue alteration and a successful esthetic outcome. The surgical protocol used allowed the ideal three-dimensional placement of the implant with the restoration of the bone buccal wall, favoring the esthetic and functional outcome of the case with harmony between white and pink esthetics. In conclusion, the employed treatment validated immediate implant-supported restoration of the missing tooth with high predictability. Furthermore, this protocol resulted in fewer surgical interventions, regeneration, and preservation of peri-implant tissues reaching the patient’s expectations