ポリテトラフルオロエチレン フィルム ニ ヨル スイヨウエキチュウ ノ ムデンカ キンゾク サクタイ ノ ノウシュク

ポリテトラフルオロエチレン(PTFE)製シールテープ(幅1.3cm、厚み0.1mm、長さ20cm)を浮かべた約500mlの試料水溶液中にクロロホルム蒸気を30分間通気・循環し、その後、ピンセットでテープを取り出し、有機溶媒に浸してテープに捕そくされている溶質を溶離した。溶質として、水銀(II)、鉛(II)、銀(I)及びカドミウム(II)のジチゾン錯体並びにコバルト(II)、ニッケル(II)、カドミウム(II)及び亜鉛(II)のPAN{1-(2-ピリジルアゾ)-2-ナフトール|錯体を用いたところ、良好な捕そく率が得られた。本法は単色法によるジチゾン錯体の定量にも適用できる。カドミウム(II)-PAN錯体ではクロロホルムの代わりにベンゼン蒸気を、又、亜鉛(II)-PAN錯体ではクロロホルム蒸気を1時間通気・循環した。A PTFE-seal-tape of 20 cm long, 1.3 cm wide and 0.1 mm thick was placed on the surface of an aq. solution of 400 to 500 ml. Chloroform vapor was introduced into the solution and circulated in a closed system for 30 min. Then, the film was picked up and the solute held on it was eluted with CHCl3. As solutes, Hg(II), Pb(II), Ag(I) and Cd(II)-dithizonates and Co(II), Ni(II), Gd(II) and Zn(II)-PAN complexes were used (dithizone: diphenylthiocarbazone; PAN: l-(2-pyridylazo)-2-naphthol). For Cd(II)-PAN benzene vapor was circulated, and for Zn(II)-PAN, CHCl3 vapor was circulated for 1 h. Recoveries of metal dithizonates and PAN complexes were more than 80%. In an acidic solution, free dithizone is collected almost completely by the PTFE film, but can be re- B moved from the film while keeping metal dithizonates on the film by circulating CHCl3 vapor in an alkaline solution. When air or carbon tetrachloride vapor was circulated, metal complexes were not collected by the PTFE film. The PTFE film surface is assumed to be covered with a thin layer of liquid CHCl3 upon circulating CHCl3 vapor in aq. solution. The present method is applicable to solutes with a large　chloroform/water partition constant

Study on variations in the normal microflora of the pharynx and the serum immunoglobulins in members of the 21st Japanese Antarctic Research Expedition 1979-1981

We examined the difference between the normal microflora in the pharynx and the levels of serum immunoglobulins of the Expedition members during their stay in Japan and at Syowa and Mizuho Stations, Antarctica. 1) There was no evidence that the bacterial species and the number of microflora in the pharynx had changed during the first three months at Syowa Station. 2) The number of pathogenic bacteria in the pharynx increased in the middle of winter (June and July) at Syowa Station. 3) In midsummer (November and December) of Antarctica, the pathogenic bacteria in the pharynx increased, probably due to the outdoor work. 4) The pathogenic bacteria were not detected in the pharynx of the members living at Mizuho Station, the nearest station to the South Pole, where the mercury falls to about 30 to 40 degrees below zero for eight months. 5) No remarkable differences were recognized in the levels of IgG and IgM in serum of the members during their stay in Japan and in Antarctica. On the other hand, IgA of them showed a slight increase, which may be explained by enhanced activity of local immunity

ダイ21ジ エットウ タイイン ノ イントウ セイジョウ サイキンソウ ト タイエキ セイ メンエキ グロブリン コウタイ ノ スイイ ニ ツイテ

環境的に汚染度の高い日本国内と, 汚染度の少ないと思われる南極において, 同一個体が生活を続けた場合の咽頭の正常細菌叢, および体液性免疫グロブリン抗体の変動を検討する目的で本実験を行った。方法として出発前に隊員より咽頭分泌物を採取し細菌培養を行い対照とし, 現地では季節の変わり目などに数回, さらに帰国後3ヵ月を経て同様に検索した。また血清を採取して, 免疫グロブリンIgG, IgA, IgMについても測定した。その結果は, 以下のとおりである。1) 現地で3ヵ月位の生活では, 咽頭部の正常細菌叢にあまり変動はみられない。2) 現地における冬季には, 病原菌を検出するものが増加していた。3) みずほ基地での生活者は, 4名中3名が正常細菌叢のバランスを保っていた。4) 現地の夏季には病原菌の検出率も高い。5) 帰国後の検索では病原菌が多数認められる。6) 体液性免疫グロブリンでは, IgAが増加していた。We examined the difference between the normal microflora in the pharynx and the levels of serum immunoglobulins of the Expedition members during their stay in Japan and at Syowa and Mizuho Stations, Antarctica. 1) There was no evidence that the bacterial species and the number of microflora in the pharynx had changed during the first three months at Syowa Station. 2) The number of pathogenic bacteria in the pharynx increased in the middle of winter (June and July) at Syowa Station. 3) In midsummer (November and December) of Antarctica, the pathogenic bacteria in the pharynx increased, probably due to the outdoor work. 4) The pathogenic bacteria were not detected in the pharynx of the members living at Mizuho Station, the nearest station to the South Pole, where the mercury falls to about 30 to 40 degrees below zero for eight months. 5) No remarkable differences were recognized in the levels of IgG and IgM in serum of the members during their stay in Japan and in Antarctica. On the other hand, IgA of them showed a slight increase, which may be explained by enhanced activity of local immunity