Expression of survivin detected by immunohistochemistry in the cytoplasm and in the nucleus is associated with prognosis of leiomyosarcoma and synovial sarcoma patients

<p>Abstract</p> <p>Background</p> <p>Survivin, a member of the inhibitor of apoptosis-protein family suppresses apoptosis and regulates cell division. It is strongly overexpressed in the vast majority of cancers. We were interested if survivin detected by immunohistochemistry has prognostic relevance especially for patients of the two soft tissue sarcoma entities leiomyosarcoma and synovial sarcoma.</p> <p>Methods</p> <p>Tumors of leiomyosarcoma (n = 24) and synovial sarcoma patients (n = 26) were investigated for their expression of survivin by immunohistochemistry. Survivin expression was assessed in the cytoplasm and the nucleus of tumor cells using an immunoreactive scoring system (IRS).</p> <p>Results</p> <p>We detected a survivin expression (IRS > 2) in the cytoplasm of 20 leiomyosarcomas and 22 synovial sarcomas and in the nucleus of 12 leiomyosarcomas and 9 synovial sarcomas, respectively. There was no significant difference between leiomyosarcoma and synovial sarcoma samples in their cytoplasmic or nuclear expression of survivin. Next, all sarcoma patients were separated in four groups according to their survivin expression in the cytoplasm and in the nucleus: group 1: negative (IRS 0 to 2); group 2: weak (IRS 3 to 4); group 3: moderate (IRS 6 to 8); group 4: strong (IRS 9 to 12). In a multivariate Cox's regression hazard analysis survivin expression detected in the cytoplasm or in the nucleus was significantly associated with overall survival of patients in group 3 (RR = 5.7; P = 0.004 and RR = 5.7; P = 0.022, respectively) compared to group 2 (reference). Patients whose tumors showed both a moderate/strong expression of survivin in the cytoplasm and a moderate expression of survivin in the nucleus (in both compartments IRS ≥ 6) possessed a 24.8-fold increased risk of tumor-related death (P = 0.003) compared to patients with a weak expression of survivin both in the cytoplasm and in the nucleus.</p> <p>Conclusion</p> <p>Survivin protein expression in the cytoplasma and in the nucleus detected by immunohistochemistry is significantly associated with prognosis of leiomyosarcoma and synovial sarcoma patients.</p

Fifth European Dirofilaria and Angiostrongylus Days (FiEDAD) 2016

Peer reviewe

Influence of circulating prolactin increased by a psychotropic drug on gonadotrophin and progesterone secretion

The purpose of the present communication is to report on the effects of high levels of circulating prolactin on gonadotropin and progesterone secretion during the luteal phase of the menstrual cycle. Purified human prolactin not being available, endogenous prolactin was increased in six normal cycling women by daily oral administration of 150 mg sulpiride starting at midcycle. Blood samples were collected daily during a control cycle and during the cycle under treatment. Serum luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin were measured by radioimmunoassay methods. Prolactin results were expressed in terms of a laboratory standard: 1.0 unit (U) is the amount of immunoreactive prolactin contained in 1.0 ml of a pool of sera rich in prolactin. Serum progesterone was measured by competitive protein binding. All control cycles were ovulatory. The length of the luteal phase ranged from 14 to 15 days. In all cycles under treatment, there was a significant rise (p < 0.001) in serum prolactin starting the day of sulpiride administration and sustained during the entire luteal phase: the mean serum prolactin was 790 mU/ml as compared to 120 mU/ml during the luteal phase of the control cycles. Two cases showed no LH surge and no rise in progesterone secretion. In three out of the other four cases, the LH peaks occurred under treatment; they were lower than in the corresponding control cycles. In the four ovulatory cycles, the length of the luteal phase ranged from 11 to 14 days and was shortened by 1 to 4 days. During the luteal phase, the mean levels of serum LH (2.5 mIU/ml), FSH (5.0 mIU/ml) and progesterone (0.009 μg/ml) were significantly lower (p < 0.001) than those of the control cycles (LH = 7.4 mIU/ml; FSH = 10.0 mIU/ml; progesterone = 0.012 μg/ml).SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe

EFFETS DE L'HYPERPROLACTINEMIE INDUITE PAR LE SULPIRIDE SUR LES SECRETIONS DE GONADOTROPHINES ET DE PROGESTERONE

SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe

Evolution des gonadotrophines et de la progestérone sériques au cours d'une hyperprolactinémie induite par le sulpiride (Dogmatil R) pendant la phase lutéale du cycle menstruel

info:eu-repo/semantics/publishe

Oral contraception can decrease the pituitary capacity to release gonadotrophins in response to synthetic LH-releasing-hormone

The amounts of luteinizing hormone (LH) and follicle stimulating hormone (FSH) released in response to the intravenous administration of 12.5, 25, and 100 μg of LH-Releasing Hormone (LH-RH) were found to be severely impeded in 4 out of 5 women who had been taking oral contraceptives for periods ranging from 12 to 36 months. Long-term administration of oral contraceptives seems to limit the reserve capacity of the pituitary to release gonadotrophins. © 1974 J. F. Bergmann.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

EVOLUTION DES GONADOTROPHINES ET DE LA PROGESTéRONE SéRIQUES AU COURS D'UNE HYPERPROLACTINéMIE INDUITE PAR LE SULPIRIDE (DOGMATIL R) PENDANT LA PHASE LUTéALE DU CYCLE MENSTRUEL

info:eu-repo/semantics/publishe

Le Troisième Reich dans l’historiographie allemande

Confrontée à un passé bien lourd, d'abord privée de sources, l'historiographie allemande s’est libérée peu à peu de la perception qu’avaient eue les contemporains des réalités du Troisième Reich. Comme toute gestion mémorielle des crises graves et des époques criminelles, l’histoire de la période 1933-1945 fut d’abord écrite en marge d’une opinion plus soucieuse de tourner la page que de se souvenir. En mettant spectaculairement en évidence la responsabilité des fonctionnaires, les grands procès des années 1960 (Eichmann, Einsatzgruppen, Auschwitz) alimentèrent la contestation par la jeune génération du passé de leurs pères. Des fictions, des polémiques relayées par les médias et des expositions spectaculaires contribuèrent à la prise de conscience. Tel fut par exemple le cas de la présentation au grand public des crimes de la Wehrmacht, qui détruisit le mythe d’une armée noble comparée à des SS responsables de tous les maux. Las d’une république en crise endémique l’électorat du Reich avait attendu des solutions miracles d’un homme providentiel. Mais selon une formule célèbre, les Allemands de 1932 n’ont voté ni pour la guerre, ni pour Auschwitz. Ils ont pourtant eu l’un et l’autre – et le nazisme en fit des instruments de son pouvoir. Quand ils en prirent conscience, il était trop tard. L’impossibilité d’agir autrement ne fut pas la seule raison de l’adhésion au régime jusque dans sa dimension criminelle