Enhancement of Power Efficiency in OFDM System by SLM with Predistortion Technique, Journal of Telecommunications and Information Technology, 2011, nr 4

Orthogonal Frequency Division Multiplexing (OFDM) is considered as a strong candidate for future wireless communication because it is marked by its higher frequency multiplicity and greater immunity to multipath fading. However, the main drawback of OFDM is its high amplitude fluctuations measured by peak-to-average power ratio (PAPR), which leads to power inefficiency and requires expensive high power amplifier (HPA) with very good linearity. In this paper, we propose selected mapping (SLM) with predistortion technique to decrease the nonlinear distortion and to improve the power efficiency of the nonlinear HPA. In the proposed method SLM reduces the PAPR and improves the power efficiency, the predistorter improves the bit error rate (BER) performance of the system. The PAPR reduction is possible with SLM when compared with original OFDM. After reducing the PAPR with SLM the data goes into the HPA with and without predistorter. The BER performance curves of SLM method with or without predistorter shows that, predistorter operates more effectively in SLM method than original OFDM system. At 4 dB IBO (input backoff) the conventional method with predistorter achieves 1.8 dB SNR gain than conventional method without a predistorter and at 6 dB IBO the BER performance is towards the ideal linear amplifier. The proposed system will be evaluated for OFDM system in the presence of a nonlinear power amplifier

Pan-RAF and MEK vertical inhibition enhances therapeutic response in non-V600 BRAF mutant cells

BACKGROUND: Currently, there are no available targeted therapy options for non-V600 BRAF mutated tumors. The aim of this study was to investigate the effects of RAF and MEK concurrent inhibition on tumor growth, migration, signaling and apoptosis induction in preclinical models of non-V600 BRAF mutant tumor cell lines. METHODS: Six BRAF mutated human tumor cell lines CRL5885 (G466 V), WM3629 (D594G), WM3670 (G469E), MDAMB231 (G464 V), CRL5922 (L597 V) and A375 (V600E as control) were investigated. Pan-RAF inhibitor (sorafenib or AZ628) and MEK inhibitor (selumetinib) or their combination were used in in vitro viability, video microscopy, immunoblot, cell cycle and TUNEL assays. The in vivo effects of the drugs were assessed in an orthotopic NSG mouse breast cancer model. RESULTS: All cell lines showed a significant growth inhibition with synergism in the sorafenib/AZ628 and selumetinib combination. Combination treatment resulted in higher Erk1/2 inhibition and in increased induction of apoptosis when compared to single agent treatments. However, single selumetinib treatment could cause adverse therapeutic effects, like increased cell migration in certain cells, selumetinib and sorafenib combination treatment lowered migratory capacity in all the cell lines. Importantly, combination resulted in significantly increased tumor growth inhibition in orthotropic xenografts of MDAMB231 cells when compared to sorafenib - but not to selumetinib - treatment. CONCLUSIONS: Our data suggests that combined blocking of RAF and MEK may achieve increased therapeutic response in non-V600 BRAF mutant tumors

Enhancement of Power Efficiency in OFDM System by SLM with Predistortion Technique

Orthogonal Frequency Division Multiplexing (OFDM) is considered as a strong candidate for future wireless communication because it is marked by its higher frequency multiplicity and greater immunity to multipath fading. However, the main drawback of OFDM is its high amplitude fluctuations measured by peak-to-average power ratio (PAPR), which leads to power inefficiency and requires expensive high power amplifier (HPA) with very good linearity. In this paper, we propose selected mapping (SLM) with predistortion technique to decrease the nonlinear distortion and to improve the power efficiency of the nonlinear HPA. In the proposed method SLM reduces the PAPR and improves the power efficiency, the predistorter improves the bit error rate (BER) performance of the system. The PAPR reduction is possible with SLM when compared with original OFDM. After reducing the PAPR with SLM the data goes into the HPA with and without predistorter. The BER performance curves of SLM method with or without predistorter shows that, predistorter operates more effectively in SLM method than original OFDM system. At 4 dB IBO (input backoff) the conventional method with predistorter achieves 1.8 dB SNR gain than conventional method without a predistorter and at 6 dB IBO the BER performance is towards the ideal linear amplifier. The proposed system will be evaluated for OFDM system in the presence of a nonlinear power amplifier

High-Fidelity Reprogrammed Human IPSCs Have a High Efficacy of DNA Repair and Resemble hESCs in Their MYC Transcriptional Signature

Human induced pluripotent stem cells (hiPSCs) are reprogrammed from adult or progenitor somatic cells and must make substantial adaptations to ensure genomic stability in order to become “embryonic stem cell- (ESC-) like.” The DNA damage response (DDR) is critical for maintenance of such genomic integrity. Herein, we determined whether cell of origin and reprogramming method influence the DDR of hiPSCs. We demonstrate that hiPSCs derived from cord blood (CB) myeloid progenitors (i.e., CB-iPSC) via an efficient high-fidelity stromal-activated (sa) method closely resembled hESCs in DNA repair gene expression signature and irradiation-induced DDR, relative to hiPSCs generated from CB or fibroblasts via standard methods. Furthermore, sa-CB-iPSCs also more closely resembled hESCs in accuracy of nonhomologous end joining (NHEJ), DNA double-strand break (DSB) repair, and C-MYC transcriptional signatures, relative to standard hiPSCs. Our data suggests that hiPSCs derived via more efficient reprogramming methods possess more hESC-like activated MYC signatures and DDR signaling. Thus, an authentic MYC molecular signature may serve as an important biomarker in characterizing the genomic integrity in hiPSCs