カゼイン摂取マウス腸管内で生成するホスホペプチドのアミノ酸組成

著者らは既に,牛乳カルシウムの利用性が高いことの理由のひとつに,小腸管内に生成するカゼインホスホペプチドが考えられることを指摘した.その機構を知る第一歩として本研究では,カゼイン摂取マウスの小腸管内容物からホスホペプチドを単離し,アミノ酸組成を調べた. マウスには体重130～200gの雄ウイスター種を用い,β-カゼインもしくはHammarstenカゼインを含む飼料をspace-feedingし,その腸内容物からホスホペプチドを分離した.ホスホペプチドの単離並びに精製はゲル泳過法およびDowex50イナン交換クロマトグラフィーによった.またアミノ酸組成は,ホスホペプチドの加水分解物について日立アミノ酸自動分析器034型によって分析した. 単離したホスホペプチドは,グルタミン酸とホスホセリンが多く,これらがCa結合性残基となる可能性が考えられた.またN/P比は,トリプシン消化ペプチドについての文献値に近似していた

On the quality of raw milk utilized in Okayama prefacture. : Chemical components.

Chemical investigation of milk which had been consumed in Okayama prefacture from Agust in 1956 to June 1957 has been done. About 170 samples were collected at 10 regions (A-J in Fig. 1), and pH, titratable acidity, fat content by Gerber method, protein content by Kjedahl method, lactose content by Lane-Eynone method and milk solid of them were determined. Results obtained are summarised as follows; 1) The great part of samples were within the range of 3.20～3.70% of fat, 2.60～3.30% of protein, 4.10～4.40% of lactose, and 0.13～0.16% of acidity, respectively. On the average of them, the fat content was higher than that of whole land and the lactose content was slightly lower. 2) In summer, the decrease of protein content was remarkedly but the fat content was even in all seasons

On the quality of raw milk utilized in Okayama prefacture. : Bacterial count.

Bacterial investigation has been performed as the previous report. Coli form bacteria was determined by positive Presumptive test with desoxycholate agar. Total bacterial count was represented with individual count (IMC) and clump count (CMC) by direct microscopic method. The ratio of IMC/CMC was calculated to obtained some knowledges of bacterial growth. As the results, it was ascertained that the bacterial count of these samples and the contamination of coli form bacteria were remarkedly. Milk temperature was almost equal to the atmospheric temperature. From this, we concluded that the cooling of milk, especially at farmers, was important to improve the bacteriological quality of milk at this area

Studies on the Raw Milk utilized in Okayama Prefecture. : The Milk-solids Calculating Formula.

Statistical investigations of various milk-solids calculating formulas had been performed with 228 samples of Holstein's milk during June in 1956 to Dec. in 1958. The specific gravities and fat contents of them lowered slightly in summar, but their SNF contents decreased remarkedly in the season. Then, significant difference was also observed in summar between the calculated and determined milk-solids contents. This fact was resulted from the lowering of SNF content, so the use of correcting factor was neccessary to obtained better results. Similar results were observed in October's samples. There were no significant differences in the chemical components of milks colected from the older, pre-war, experienced farmers and unaccustomed new ones

Isokinetic Closed Kinetic Chain Exercise における膝関節反力の筋骨格モデル解析

前十字靱帯損傷者の術後リハビリテーションとして閉運動連鎖(CKC)が注目されてきた。CKCで理想的な下肢全体の筋収縮を引き出すためにサイクロイド曲線を利用した等運動性閉運動連鎖型評価訓練機を開発した。足部が円弧状運動を行うことで股関節、膝関節に均等にトルクを分散しスムーズで安全な運動が可能となる。ロードセルによる足部出力の計測と、ビデオキャプチャーデータを同期して各関節トルクを求めることができる。この運動について筋骨格シミュレーションモデルを用いて解析し、膝関節に加わるストレスを検討した。健常人では本機により大腿四頭筋とハムストリングのバランスの良い収縮が得られ、膝関節全屈曲角度域にわたって脛骨を前方へ引き出す力はほとんど働いていないことが証明された。CYBEX などのOKC では再建靱帯に危険なストレスがかかる可能性があり、本機によるCKC での筋力評価訓練は安全かつ有用であると考える