9 research outputs found
Design, synthesis, and biological evaluation of bone-targeted proteasome inhibitors for multiple myeloma
Oxidation of hemoglobin and redistribution of band 3 promote erythrophagocytosis in visceral leishmaniasis
In visceral leishmaniasis (VL), oxidative
assault on erythrocytes perturbs their cellular environment
and makes them vulnerable to premature hemolysis. In this
study, we assessed the contribution of oxidation-induced
modifications of hemoglobin and membrane protein band 3
in the reduced survival of red cells in VL. Oxidative
transformation of oxyhemoglobin to hemichrome enhanced
its interaction with erythrocyte membrane in the infected
animals. Association between denatured globin and band 3
contributed to the formation of insoluble copolymer of
macromolecular dimension. Disulfide bonding appeared to
be necessary in the making of high molecular weight
aggregates during copolymerization. Hemichrome induced
clustering of band 3 promoted generation of epitopes on
erythrocyte cell surface. This provided a signal favoring
immunologic recognition of redistributed band 3 by
autologous IgG followed by erythrophagocytosis. An
eventual outcome of the sequence of events pointed to
early removal of affected red cells from circulation during
the disease