InSight's Reconstructed Aerothermal Environments

The InSight spacecraft was proposed to be a build-to-print copy of the Phoenix vehicle due to the knowledge that the lander payload would be similar and the trajectory would be similar. However, the InSight aerothermal analysts, based on tests performed in CO2 during the Mars Science Laboratory mission (MSL) and completion of Russian databases, considered radiative heat flux to the aftbody from the wake for the first time for a US Mars mission. The combined convective and radiative heat flux was used to determine if the as-flown Phoenix thermal protection system (TPS) design would be sufficient for InSight. All analyses showed that the design would be adequate. Once the InSight lander was successfully delivered to Mars on November 26, 2018, work began to reconstruct the atmosphere and trajectory in order to evaluate the aerothermal environments that were actually encountered by the spacecraft and to compare them to the design environments.The best estimated trajectory (BET) reconstructed for the InSight atmospheric entry fell between the two trajectories considered for the design, when looking at the velocity versus altitude values. The maximum heat rate design trajectory (MHR) flew at a higher velocity and the maximum heat load design trajectory (MHL) flew at a lower velocity than the BET. For TPS sizing, the MHL trajectory drove the design. Reconstruction has shown that the BET flew for a shorter time than either of the design environments, hence total heat load on the vehicle should have been less than used in design. Utilizing the BET, both DPLR and LAURA were first run to analyze the convective heating on the vehicle with no angle of attack. Both codes were run with axisymmetric, laminar flow in radiative equilibrium and vibrational non-equilibrium with a surface emissivity of 0.8. Eight species Mitcheltree chemistry was assumed with CO2, CO, N2, O2, NO, C, N, and O. Both codes agreed within 1% on the forebody and had the expected differences on the aftbody. The NEQAIR and HARA codes were used to analyze the radiative heating on the vehicle using full spherical ray-tracing. The codes agreed within 5% on most aftbody points of interest.The LAURA code was then used to evaluate the conditions at angle of attack at the peak heating and peak pressure times. Boundary layer properties were investigated to confirm that the flow over the forebody was laminar for the flight.Comparisons of the aerothermal heating determined for the reconstructed trajectory to the design trajectories showed that the as-flown conditions were less severe than desig

Clostridium difficile in Retail Meat Products, USA, 2007

To determine the presence of Clostridium difficile, we sampled cooked and uncooked meat products sold in Tucson, Arizona. Forty-two percent contained toxigenic C. difficile strains (either ribotype 078/toxinotype V [73%] or 027/toxinotype III [NAP1 or NAP1-related; 27%]). These findings indicate that food products may play a role in interspecies C. difficile transmission

Genome Sequencing and Analysis of a Type A Clostridium perfringens Isolate from a Case of Bovine Clostridial Abomasitis

Clostridium perfringens is a common inhabitant of the avian and mammalian gastrointestinal tracts and can behave commensally or pathogenically. Some enteric diseases caused by type A C. perfringens, including bovine clostridial abomasitis, remain poorly understood. To investigate the potential basis of virulence in strains causing this disease, we sequenced the genome of a type A C. perfringens isolate (strain F262) from a case of bovine clostridial abomasitis. The ∼3.34 Mbp chromosome of C. perfringens F262 is predicted to contain 3163 protein-coding genes, 76 tRNA genes, and an integrated plasmid sequence, Cfrag (∼18 kb). In addition, sequences of two complete circular plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), and two incomplete plasmid fragments, pF262A (48.5 kb) and pF262B (50.0 kb), were identified. Comparison of the chromosome sequence of C. perfringens F262 to complete C. perfringens chromosomes, plasmids and phages revealed 261 unique genes. No novel toxin genes related to previously described clostridial toxins were identified: 60% of the 261 unique genes were hypothetical proteins. There was a two base pair deletion in virS, a gene reported to encode the main sensor kinase involved in virulence gene activation. Despite this frameshift mutation, C. perfringens F262 expressed perfringolysin O, alpha-toxin and the beta2-toxin, suggesting that another regulation system might contribute to the pathogenicity of this strain. Two complete plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), unique to this strain of C. perfringens were identified

Bennu regolith mobilized by TAGSAM: Expectations for the OSIRIS-REx sample collection event and application to understanding naturally ejected particles

The Origins, Spectral Interpretation, Resource Identification, and Security–Regolith Explorer (OSIRIS-REx) mission will collect material from the asteroid Bennu and return it to Earth. The sample collection method uses pressurized nitrogen gas to mobilize regolith. It is likely that the gas will mobilize more, potentially much more, material than is captured by the Touch-and-Go Sample Acquisition Mechanism (TAGSAM) for return. The amount, velocities, and fate of this mobilized material have important implications for our understanding of Bennu's physical properties and processes, such as impact cratering, regolith mobility, and the recently observed natural ejection of particles. The nature of the TAGSAM-ejected material is also significant for mission operations. We conducted numerical simulations and ground-based tests to estimate the amount and speeds of material mobilized by the sampling event. The estimated ejected masses range from 12 kg to >165 kg; given that Bennu appears to be a nearly strengthless rubble pile, the higher end of the mass range is more likely. Maximum ejection speeds for 1-cm-diameter particles could reach 10 m/s; smaller particles will be ejected at faster speeds. Minimum ejection speeds will be determined by the as yet unknown cohesive strength of Bennu's regolith. Material ejected more slowly than 10 cm/s will reaccrete on Bennu; material ejected between 10 and 30 cm/s may or may not reaccrete; and material ejected faster than 30 cm/s will escape. Some of the ejected material is predicted to remain in transient orbits around Bennu. The lifetimes of these orbits span from days to several weeks, the maximum duration of our integrations. Reaccreted material should be concentrated in a region near the sample site, although re-impacts will occur globally. Gas from TAGSAM will mobilize material beyond the contact perimeter; because of interactions between cohesion and microgravity, there is a preferred particle size of mobility, predicted to be tens of centimeters, depending on the TAG latitude. Observing Bennu during and after the sampling event will make it possible to test these predictions and understand how the asteroid responds to disruption and particle mobility across the surface

Zoonotic Transfer of Clostridium difficile Harboring Antimicrobial Resistance between Farm Animals and Humans.

The emergence of Clostridium difficile as a significant human diarrheal pathogen is associated with the production of highly transmissible spores and the acquisition of antimicrobial resistance genes (ARGs) and virulence factors. Unlike the hospital-associated C. difficile RT027 lineage, the community-associated C. difficile RT078 lineage is isolated from both humans and farm animals; however, the geographical population structure and transmission networks remain unknown. Here, we applied whole-genome phylogenetic analysis of 248 C. difficile RT078 strains from 22 countries. Our results demonstrate limited geographical clustering for C. difficile RT078 and extensive coclustering of human and animal strains, thereby revealing a highly linked intercontinental transmission network between humans and animals. Comparative whole-genome analysis reveals indistinguishable accessory genomes between human and animal strains and a variety of antimicrobial resistance genes in the pangenome of C. difficile RT078. Thus, bidirectional spread of C. difficile RT078 between farm animals and humans may represent an unappreciated route disseminating antimicrobial resistance genes between humans and animals. These results highlight the importance of the "One Health" concept to monitor infectious disease emergence and the dissemination of antimicrobial resistance genes

Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens

Clostridium perfringens is a Gram-positive, anaerobic spore-forming bacterium commonly found in soil, sediments, and the human gastrointestinal tract. C. perfringens is responsible for a wide spectrum of disease, including food poisoning, gas gangrene (clostridial myonecrosis), enteritis necroticans, and non-foodborne gastrointestinal infections. The complete genome sequences of Clostridium perfringens strain ATCC 13124, a gas gangrene isolate and the species type strain, and the enterotoxin-producing food poisoning strain SM101, were determined and compared with the published C. perfringens strain 13 genome. Comparison of the three genomes revealed considerable genomic diversity with >300 unique "genomic islands" identified, with the majority of these islands unusually clustered on one replichore. PCR-based analysis indicated that the large genomic islands are widely variable across a large collection of C. perfringens strains. These islands encode genes that correlate to differences in virulence and phenotypic characteristics of these strains. Significant differences between the strains include numerous novel mobile elements and genes encoding metabolic capabilities, strain-specific extracellular polysaccharide capsule, sporulation factors, toxins, and other secreted enzymes, providing substantial insight into this medically important bacterial pathogen. ©2006 by Cold Spring Harbor Laboratory Press

Identification of Novel Pathogenicity Loci in Clostridium perfringens Strains That Cause Avian Necrotic Enteritis

Type A Clostridium perfringens causes poultry necrotic enteritis (NE), an enteric disease of considerable economic importance, yet can also exist as a member of the normal intestinal microbiota. A recently discovered pore-forming toxin, NetB, is associated with pathogenesis in most, but not all, NE isolates. This finding suggested that NE-causing strains may possess other virulence gene(s) not present in commensal type A isolates. We used high-throughput sequencing (HTS) technologies to generate draft genome sequences of seven unrelated C. perfringens poultry NE isolates and one isolate from a healthy bird, and identified additional novel NE-associated genes by comparison with nine publicly available reference genomes. Thirty-one open reading frames (ORFs) were unique to all NE strains and formed the basis for three highly conserved NE-associated loci that we designated NELoc-1 (42 kb), NELoc-2 (11.2 kb) and NELoc-3 (5.6 kb). The largest locus, NELoc-1, consisted of netB and 36 additional genes, including those predicted to encode two leukocidins, an internalin-like protein and a ricin-domain protein. Pulsed-field gel electrophoresis (PFGE) and Southern blotting revealed that the NE strains each carried 2 to 5 large plasmids, and that NELoc-1 and -3 were localized on distinct plasmids of sizes ∼85 and ∼70 kb, respectively. Sequencing of the regions flanking these loci revealed similarity to previously characterized conjugative plasmids of C. perfringens. These results provide significant insight into the pathogenetic basis of poultry NE and are the first to demonstrate that netB resides in a large, plasmid-encoded locus. Our findings strongly suggest that poultry NE is caused by several novel virulence factors, whose genes are clustered on discrete pathogenicity loci, some of which are plasmid-borne

Incidence and trends of low back pain hospitalisation during military service – An analysis of 387,070 Finnish young males

<p>Abstract</p> <p>Background</p> <p>There is evidence that low back pain (LBP) during young adulthood and military service predicts LBP later in life. The purpose of this study was to investigate the incidence and trends of LBP hospitalisation among Finnish military conscripts.</p> <p>Methods</p> <p>All male conscripts performing their compulsory military service during 1990–2002 were included in the study population. Altogether 387,070 military conscripts were followed throughout their six-to-twelve-month service period. Data on LBP hospitalisations were obtained from the National Hospital Discharge Register.</p> <p>Results</p> <p>Altogether 7,240 LBP hospitalisations were identified among 5,061 (1.3%) male conscripts during the study period. The event-based incidence of LBP hospitalisation was 27.0 (95% confidence interval (CI): 25.7–28.2). In most cases, the diagnosis was unspecified LBP (<it>n </it>= 5,141, 71%) followed by lumbar disc disorders (<it>n </it>= 2,069, 29%). Hospitalisation incidence due to unspecified LBP was 19.1 per 1,000 person-years (95% CI: 18.3 to 20.4), and 7.8 per 1,000 person-years (95% CI: 6.7 to 8.3) due to lumbar disc disorders. The incidence of unspecified LBP remained unaltered, while hospitalisation due to lumbar disc disorders declined from 1993 onwards.</p> <p>Conclusion</p> <p>Although conscripts accepted into military training pass physician-performed examinations as healthy, young adults, LBP hospitalisation causes significant morbidity during military service.</p