Polymorphisms of Bovine HSP90 and Their Implications in Beef Cattle Productivity

Production of beef cattle represents a $60 billion industry in the United States (USDA, 2015). The American beef cattle industry loses an estimated$370 million annually due to heat stress (St-Pierre, 2003). As of 2003, this was equal to nearly 99 million pounds of beef lost (USDA, 2015). The average American consumed roughly 65 pounds of beef in 2003; this means that the 99 million pounds of beef lost to heat stress would have been enough to feed approximately 1.5 million Americans for an entire year (Barclay, 2012)

Operation of the T-100 Hall Effect Thruster

The introduction of Hall Effect Thrusters from the former Soviet Union to the United States generated considerable interest in using the propulsion system aboard Western spacecraft. The established programs evaluated the SPT-100, and TAL-55 Hall Effect Thrusters for efficiency, lifetime, and performance characteristics. The T-100 model garnered only minor interest during this time compared with the same generation counterparts, the SPT-100, and TAL-55. This gap in knowledge on the efficiency, and performance of the T-100 warrants investigation into the design, and operation of the thruster. Operational characteristics will be measured on a restored T-100 Hall Thruster, using argon as a propellant. The efficiency of the Hall Thruster operation is affected by the utilization of the propellant mass, and power inputs. Power inputs of the magnetic arrays for the T-100 are less than optimal due to the inherent losses in the magnetic architecture. The ignition system of the hallow cathode improves cathode mass flow efficiency by eliminating pre-heating. The T-100 Hall Effect Thruster provides a robust option for modern electric propulsion systems that is comparable to its counterparts

tmRNA - a novel high-copy-number RNA diagnostic target - its application for Staphylococcus aureus detection using real-time NASBA

A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent was determined for the assay. A chimeric in vitro transcribed internal amplification control was developed and included in the assay. Application of the assay in natural and artificially contaminated unpasteurized (raw) milk enabled detection of 1-10 CFUS. aureus mL(-1) in 3-4 h, without the need for culture enrichment. Staphylococcus aureus was detected in all artificially contaminated milk samples (n=20) and none of the natural milk samples (n=20). Microbiological analysis of the natural milk samples was performed in parallel according to ISO 6888-3 and confirmed the absence of S. aureus. The method developed in this study has the potential to enable the specific detection of S. aureus in raw milk in a significantly shorter time frame than current standard methods. The assay further demonstrates the usefulness of tmRNA/ssrA as a nucleic acid diagnostic target

A Clean, Green New Zealand? An In-Depth Look at the Personal Experiences of Animal Rights Activists

This study explored personal experiences of animal rights and environmental activists in New Zealand. The stories of participants provided insight into the challenges activists face in a country where the economy is heavily dependent on animal agriculture. A qualitative methodology was utilised and several major themes emerged: (1) emotional and psychological experiences, (2) group membership, (3) characteristics of activism and liberation, (4) the law and its agents, and (5) challenge to society. Participants of the study represent a group of individuals engaged in acts of altruistic offending triggered by exposure to the suffering of non-human animals. Their moral philosophy and conscience overrode all considerations for legal repercussions, and through their activism they not only challenged the status quo, but also called upon non-activist members of society to make meaningful contributions to the world around them

A Style Comparison of the Piano and Organ Versions of Liszt’s Prelude and Fugue on B-A-C-H

This paper analyzes the piano and organ versions of the Prelude and Fugue on B-A-C-H composed by Franz Liszt and provides a style comparison of the two. Jerry Glynn Smith also provides context for Liszt's work with the organ

Expert Views on Effectiveness, Feasibility, and Implementation of Biosecurity Measures for Mitigating Tier 1 Disease Risks in the U.S. Swine, Beef Cattle, and Dairy Industries

Understanding disease transmission routes and implications for biosecurity is critical to mitigating livestock disease outbreaks and maintaining efficient and profitable production. The goal of biosecurity is to eliminate pathogen exposure and minimize endemic pathogen impact, particularly important for foreign animal diseases that threaten U.S. animal health and the economy. We elicit swine, beef cattle, and dairy expert views on the effectiveness, feasibility, and implementation of both biosecurity measures targeting disease transmission routes and specific biosecurity measures. Biosecurity targeting direct animal-to-animal contact, semen, people, and vehicles and other fomites was identified as the most effective and feasible for the swine, beef cattle, and dairy industries. Efforts targeting airborne and arthropod transmission were ranked low for effectiveness and feasibility across all three industries. The swine industry had higher estimated implementation of biosecurity against most disease transmission routes. All-in and all-out production had the highest estimated implementation in the swine industry. In the beef cattle industry, performing daily observations by producer/employees had the highest estimated implementation. Reduced environmental viral load through pathogen reduction had the lowest estimated implementation in the swine and beef cattle industries. In the dairy industry, monitoring production records for health status changes had the highest estimated implementation, and line of separation in place for all employees entering premises had the lowest. Swine experts considered separation line for all animals entering/leaving premises and reduced environmental viral load through pathogen reduction the most and least effective and feasible, respectively. Stabilization and monitoring for affected premises and daily observations by producer/employees were identified as most effective and feasible by beef cattle and dairy experts, respectively. All-in and all-out production was rated least effective and feasible by both beef cattle and dairy experts

Repeated Restraint Stress Exposure During Early Withdrawal Accelerates Incubation of Cue-Induced Cocaine Craving

A major challenge for treating cocaine addiction is the propensity for abstinent users to relapse. Two important triggers for relapse are cues associated with prior drug use and stressful life events. To study their interaction in promoting relapse during abstinence, we used the incubation model of craving and relapse in which cue-induced drug seeking progressively intensifies (\u27incubates\u27) during withdrawal from extended-access cocaine self-administration. We tested rats for cue-induced cocaine seeking on withdrawal day (WD) 1. Rats were then subjected to repeated restraint stress or control conditions (seven sessions held between WD6 and WD14). All rats were tested again for cue-induced cocaine seeking on WD15, 1 day after the last stress or control session. Although controls showed a time-dependent increase in cue-induced cocaine seeking (incubation), rats exposed to repeated stress in early withdrawal exhibited a more robust increase in seeking behavior between WD1 and WD15. In separate stressed and control rats,equivalent cocaine seeking was observed on WD48. These results indicate that repeated stress in early withdrawal accelerates incubation of cocaine craving, although craving plateaus at the same level were observed in controls. However, 1 month after the WD48 test, rats subjected to repeated stress in early withdrawal showed enhanced cue-induced cocaine seeking following acute (24 hours) food deprivation stress. Together, these data indicate that chronic stress exposure enhances the initial rate of incubation of craving during early withdrawal, resulting in increased vulnerability to cue-induced relapse during this period, and may lead to a persistent increase in vulnerability to the relapse-promoting effects of stress

Vanishing point detection for visual surveillance systems in railway platform environments

© 2018 Elsevier B.V. Visual surveillance is of paramount importance in public spaces and especially in train and metro platforms which are particularly susceptible to many types of crime from petty theft to terrorist activity. Image resolution of visual surveillance systems is limited by a trade-off between several requirements such as sensor and lens cost, transmission bandwidth and storage space. When image quality cannot be improved using high-resolution sensors, high-end lenses or IR illumination, the visual surveillance system may need to increase the resolving power of the images by software to provide accurate outputs such as, in our case, vanishing points (VPs). Despite having numerous applications in camera calibration, 3D reconstruction and threat detection, a general method for VP detection has remained elusive. Rather than attempting the infeasible task of VP detection in general scenes, this paper presents a novel method that is fine-tuned to work for railway station environments and is shown to outperform the state-of-the-art for that particular case. In this paper, we propose a three-stage approach to accurately detect the main lines and vanishing points in low-resolution images acquired by visual surveillance systems in indoor and outdoor railway platform environments. First, several frames are used to increase the resolving power through a multi-frame image enhancer. Second, an adaptive edge detection is performed and a novel line clustering algorithm is then applied to determine the parameters of the lines that converge at VPs; this is based on statistics of the detected lines and heuristics about the type of scene. Finally, vanishing points are computed via a voting system to optimize detection in an attempt to omit spurious lines. The proposed approach is very robust since it is not affected by ever-changing illumination and weather conditions of the scene, and it is immune to vibrations. Accurate and reliable vanishing point detection provides very valuable information, which can be used to aid camera calibration, automatic scene understanding, scene segmentation, semantic classification or augmented reality in platform environments

A comparison of Qatari and Chinese cadet table tennis players: analysis of Asian championship team competition

Video analysis was performed during the Asian Cadet Table Tennis Championships to determine playing style and patterns of top and lower ranked nations. China and Qatar were chosen for analysis based on their performance during the tournament (1st/2nd playoff and 7th/8th playoff respectively). Notational analysis techniques were implemented to analyze 3 matches for each nation. Significant (p<0.05) differences were identified in serve and return placement and shot choice. Chinese players used a higher proportion of long return placement and a higher percentage of topspin shots compared to Qatari players. This preliminary study suggests that at cadet level, Chinese players exhibit a more attacking style of play than Qatari players

Implications for physical preparation of cadet male table tennis players: analysis of the 2013 championship singles final

The purpose of this case-study was to attain and describe data on the physical demands placed on elite Asian cadet male table tennis players in high-level competition. The 2013 Asian cadet singles final was notated live while the match was video recorded for further post-analysis. A typical point consisted of 4-5 shots with the player activated for 3.2 s, working for 3.3 s and resting for 17.8 s on average. The frequency of movement types was reported and typical footwork patterns were described for the most common movement types observed (step, linear jump, lateral jump and pivot). Typical footwork sequences were presented for the first two movements when serving and returning. This information could be used to inform a strength and conditioning programme for a developing cadet player seeking to replicate a playing style of an elite Asian cadet player