Recombinational Repair of a Chromosomal DNA Double Strand Break: A Dissertation

Repairing a chromosomal DNA double strand break is essential for survival and maintenance of genomic integrity of a eukaryotic organism. The eukaryotic cell has therefore evolved intricate mechanisms to counteract all sorts of genomic insults in the context of chromatin structure. Modulating chromatin structure has been crucial and integral in regulating a number of conserved repair processes along with other fundamental genomic processes like replication and transcription. The work in this dissertation has focused on understanding the role of chromatin remodeling enzymes in the repair of a chromosomal DNA double strand break by homologous recombination. This has been approached by recapitulating the biochemical formation of recombination intermediates on chromatin in vitro. In this study, we have demonstrated that the mere packaging of DNA into nucleosomal structure does not present a barrier for successful capture of homologous DNA sequences, a central step of the biochemical pathway of recombinational repair. It is only the assembly of heterochromatin-like more complex nucleo-protein structure that presents additional constraints to this key step. And, this additional constraint can be overcome by the activities of ATP-dependent chromatin remodeling enzymes. These findings have great implications for our perception of the mechanism of the recombinational repair process of a chromosomal DNA double strand break within the eukaryotic genome

Unitary sources say: It is inhibition!

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/162710/2/tjp14269.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/162710/1/tjp14269_am.pd

Revolutionizing Retail: Design and Implementation of an AI-Powered Autonomous Checkout System

The implementation of AI-Powered Autonomous Checkout System represents a significant advancement in retail technology, aimed at revolutionizing the traditional checkout experience. This paper delves into the design and operational intricacies of an innovative AI-Powered Autonomous Checkout System and the impact of such a system on both customers and retail shops. This technology uses Artificial Intelligence and Internet of Things components to recognize products accurately and automate billing, which speeds up the checkout process. This study provides a deeper knowledge of the system's operation through in-depth insights into the checkout interface, sensor integration, and system architecture. The study also addresses the possible advantages of the AI-Powered Autonomous Checkout System, such as increased productivity, precise item identification, and happier customers. This research lays the path for future developments in the field by concentrating on the design and operational aspects, which add to a thorough understanding of AI technology's implementation in retail environments

RANDOMIZED STUDY: PROPOFOL VERSUS FENTANYL-MIDAZOLAM COMBINATION FOR CONSCIOUS SEDATION DURING FIBREOPTIC NASOTRACHEAL INTUBATION

Background The ideal characteristics of the sedative and analgesic that is used for awake fiberoptic intubation are that it should be easily titrable, that it should act rapidly, that it should maintain hemodynamic stability, that it should provide sufficient amnesia, and that it should have a short-acting time because the manipulation of the airway is only required until the intubation is finished. Currently, fentanyl and midazolam are used; propofol also fits the characteristics. This study aims to determine the efficacy and safety of both agents in the conduction of awake fiberoptic intubation. Method This study was a prospective randomized study. There were a total of 25 participants in the study. All of them underwent a pre-anesthetic assessment. The patients were divided into two groups. The first group received propofol as their sedating agent and the second group received fentanyl and midazolam combination as their sedating agent. The intubation period, sedation score, intubation score, hemodynamic vitals, oxygen saturation, degree of amnesia, and degree of global acceptance were recorded. Results Both propofol and the fentanyl-midazolam combination were effective for sedation during fiberoptic intubation. Propofol had a slightly higher sedation score at 2 minutes (mean: 13.3 vs. 15.5, p < 0.05), but both groups reached similar sedation levels at 6 minutes (score: 16). Intubation scores showed no significant differences between groups (p > 0.05). Propofol provided better hemodynamic stability, with lower systolic blood pressure during stage 2 (p < 0.05). Oxygen saturation remained stable in both groups. Complete amnesia was achieved in 75% of the propofol group and 85% of the fentanyl-midazolam group, with similar global acceptance ratings (75% vs. 85%). Conclusion Propofol alone is suitable for awake fiber optic insertion for artificial ventilation. Recommendation Propofol should be considered as a sedative agent for the performance of fiber optic insertion for artificial ventilation

How can power discourses be changed?

Social policy impact is partly determined by how policy is articulated and advocated, including which values are highlighted and how. We examine the influence of policy framing and reframing on outcomes, with particular reference to policies of the Delhi state government in India that target the practices of female feticide, infanticide and neglect that underlie the ‘daughter deficit’. Using Snow and Benford’s categories for understanding reframing processes, the paper outlines and applies a ‘model’ of reframing disputed issues, derived from looking at two famous campaigns – Gandhi’s 1930 Salt March in the struggle for Indian freedom from British rule and the African- American civil rights struggle of the 1950s and 60s. It argues that ‘carrot and stick’ policy measures, such as financial incentives and legal prohibitions, to counteract the ‘daughter deficit’ must be complemented by well crafted discursive interventions

Utilization of modern temporary contraceptive methods and its predictors among reproductive-aged women in India: insights from NFHS-5 (2019–21)

Evidence from various studies on modern contraceptive methods shows that the utilization varies greatly. The present study aimed to estimate the magnitude and determinants for temporary modern contraceptive utilization among reproductive-aged (15-49 years) women in India. We analysed National Family Health Survey-5 data using the “svyset” command in STATA software. Modern contraception utilization was estimated using the weighted prevalence, and its correlates were assessed by multivariable regression by reporting an adjusted prevalence ratio (aPR) with 95% confidence interval (CI). QGIS 3.2.1 software was used for spatial analysis of different temporary modern contraceptives. The mean (SD) age of 359,825 respondents was 31.6 (8.5) years with 75.1% (n = 270,311) and 49.2% (n = 177,165) of them being from rural area and having completed education up to secondary school, respectively. The overall utilization of modern temporary contraception was 66.1% [95%CI: 65.90–66.35, n = 237,953]. Multigravida (vs. nulligravida) [aPR = 2.13 (1.98–2.30)], higher education of husband (vs. not educated) [aPR = 1.20 (1.14–1.27)], urban (vs. rural) [aPR = 1.06 (1.03–1.10)], watching television less than once a week (vs. not at all) [aPR = 1.04 (1.01–1.08)], divorced (vs. married) [aPR = 0.65 (0.45–0.94)], and Scheduled Tribe (ST) (vs. unreserved) [aPR = 0.92 (0.88–0.96)] were significant independent determinants. The highest utilization of male condoms, IUCDs, pills and injections were in Himachal Pradesh (86%), Nagaland (64%), Tripura (85%), and Ladakh (20%), respectively. Out of every ten reproductive-aged (15–49 years) women in India, six are using temporary modern contraceptive methods. More intervention strategies should be planned, considering factors like gravida, education, residence, health promotion and caste to attain replacement fertility level