Luminescence investigations at Quendale (Broo Peninsula, Shetland)

This report is concerned with optically stimulated luminescence (OSL) investigations of sediment samples collected from ongoing University of Southern Maine archaeological excavations at the Links of Quendale, southern Shetland, investigating the early-modern township of Broo. 11 sediment samples were submitted to the luminescence laboratory at SUERC for OSL dating by Ian Simpson. This report summaries the protocols, and laboratory analysis, employed in quartz single aliquot regenerative (SAR) OSL dating, as used to construct an OSL chronology for wind-blown sands in proximity to the Broo excavations, in association with archaeological structures (5 samples), and for sands in the coastal and inland dune systems (6 samples). The chronology established for the inland sands, in contexts associated with the Broo 2 building and enclosure, spans from AD1540 ± 40 (SUTL2441) through to AD1810 ± 25 (SUTL2519), encompassing the archaeological period of interest. The dates obtained for sands within the enclosed and unenclosed areas to the immediate east and southwest of the excavated Broo site, are AD1760 ± 30 - AD1760 ± 25, and AD1810 ± 25 (SUTL2517-2518 and 2519, respectively), are consistent with the expectation that the clean sands which infill these structures, post-date the period in which the Broo township was abandoned. The coastal sand accumulations, as so far dated, yielded luminescence ages of 2380 ± 230 BC (SUTL2526), 1510 ± 270 BC (SUTL2527), AD 1030 ± 80 (SUTL2528), AD 1690 ± 50 (SUTL2529), AD 1720 ± 20 (SUTL2530) and a mixed-age sample with youngest component at AD 1955 ± 15 (SUTL2531), implying periods of sand mobilisation, synchronous with sand deposition in Orkney and northern Scotland, in the late Neolithic, the Early Bronze Age, the Norse period, the early-modern, and modern periods. This work suggests that the present-day physio-geographical setting of the Quendale Links, comprised of the coastal sand barrier, and the inland dune fields, is a product of a prolonged history of sand mobilisation, erosion and deposition from the Neolithic to the present day. Furthermore, the emerging temporal framework, coupled with the spatial distribution of dune forms across the Links, raises questions as to whether Little Ice Age storms were responsible for deposition, or erosive destruction of older dune-forms, and the re-mobilisation of this sediment. To test these ideas, profiling methods, both field- and laboratory- based, could be employed to obtain a more complete temporal and spatial characterisation of the dune systems and excavated sequences. Further OSL sampling and dating would be needed to define the vertical and lateral chronostratigraphies of the environmental features in the landscape and their relationships to archaeological structures

Intrapartum epidural analgesia and breastfeeding: a prospective cohort study

BACKGROUND Anecdotal reports suggest that the addition of fentanyl (an opioid) to epidural analgesia for women during childbirth results in difficulty establishing breastfeeding. The aim of this paper is to determine any association between epidural analgesia and 1) breastfeeding in the first week postpartum and 2) breastfeeding cessation during the first 24 weeks postpartum. METHODS A prospective cohort study of 1280 women aged > or = 16 years, who gave birth to a single live infant in the Australian Capital Territory in 1997 was conducted. Women completed questionnaires at weeks 1, 8, 16 and 24 postpartum. Breastfeeding information was collected in each of the four surveys and women were categorised as either fully breastfeeding, partially breastfeeding or not breastfeeding at all. Women who had stopped breastfeeding since the previous survey were asked when they stopped. RESULTS In the first week postpartum, 93% of women were either fully or partially breastfeeding their baby and 60% were continuing to breastfeed at 24 weeks. Intrapartum analgesia and type of birth were associated with partial breastfeeding and breastfeeding difficulties in the first postpartum week (p < 0.0001). Analgesia, maternal age and education were associated with breastfeeding cessation in the first 24 weeks (p < 0.0001), with women who had epidurals being more likely to stop breastfeeding than women who used non-pharmacological methods of pain relief (adjusted hazard ratio 2.02, 95% CI 1.53, 2.67). CONCLUSION Women in this cohort who had epidurals were less likely to fully breastfeed their infant in the few days after birth and more likely to stop breastfeeding in the first 24 weeks. Although this relationship may not be causal, it is important that women at higher risk of breastfeeding cessation are provided with adequate breastfeeding assistance and support.Christine Roberts is supported by a National Health and Medical Research Council (NHMRC) of Australia Public Health Practitioner Fellowship and Siranda Torvaldsen is supported by a NHMRC Australian Research Training Fellowship. The cohort study was supported by a project grant from The Canberra Hospital Private Practice Fund. Additional funding was provided by The Canberra Hospital Auxiliary, the Nurses' Board of the Australian Capital Territory, and the Australian Capital Territory Department of Health & Community Care

RNA-Sequencing data supports the existence of novel VEGFA splicing events but not of VEGFAxxxb isoforms

AbstractVascular endothelial growth factor (VEGFA), a pivotal regulator of angiogenesis and valuable therapeutic target, is characterised by alternative splicing which generates three principal isoforms, VEGFA121, VEGFA165 and VEGFA189. A second set of anti-angiogenic isoforms termed VEGFAxxxb that utilise an alternative splice site in the final exon have been widely reported, with mRNA detection based principally upon RT-PCR assays. We sought confirmation of the existence of the VEGFAxxxb isoforms within the abundant RNA sequencing data available publicly. Whilst sequences derived specifically from each of the canonical VEGFA isoforms were present in many tissues, there were no sequences derived from VEGFAxxxb isoforms. Sequencing of approximately 50,000 RT-PCR products spanning the exon 7–8 junction in 10 tissues did not identify any VEGFAxxxb transcripts. The absence or extremely low expression of these transcripts in vivo indicates that VEGFAxxxb isoforms are unlikely to play a role in normal physiology. Our analyses also revealed multiple novel splicing events supported by more reads than previously reported for VEGFA145 and VEGFA148 isoforms, including three from novel first exons consistent with existing transcription start site data. These novel VEGFA isoforms may play significant roles in specific cell types.</jats:p