Genetic Characterization and Bottleneck Demographic Assessment of Caspian Horse Population (KARAKTERISASI GENETIK DAN HAMBATAN DEMOGRAFI PADA PENILAIAN POPULASI KUDA KASPIA)

The aim of this study was to evaluate genetic characterization of the Caspian horse population using microsatellite markers. This study was determined the efficiency of microsatellite markers for conservation plans and breeding strategies in Caspian horse population. A total of 120 Caspian horse samples including 95 adults and 25 foals were genotyped by using seventeen microsatellite markers recommended by ISAG. The number of allele per locus varied from 5 (HMS01 and HTG07) to 9 (HTG10) with an average of 7.41. The observed heterozygosity and the expected heterozygosity ranged from 0.505-0.831 (mean 0.684), from 0.615-0.835 (mean 0.748) respectively. PIC value ranged from 0.716 (HMS01) to 0.834 (AHT04) with an average of 0.787. The total exclusion probability of 17 microsatellite loci was 0.9999. The low values of Wright’s fixation index/ Fis (0.084) indicated the low levels of inbreeding. A significant heterozygote excesses based on different models, suggested that Caspian horse population has decreased to low numbers in the past, but a bottleneck event is still  very striking, and its number has recently increased is not in mutation drift equilibrium. The present study contributes to our knowledge of the genetic diversity of the Caspian caspian horse population and helps to deûne its genetic conservation strategies

Genetic Diversity and Molecular Phylogeny of Iranian Goats Based on Cytochrome Oxidase I (COXI) Gene Sequences (KERAGAMAN GENETIK DAN FILOGENI MOLEKULER KAMBING-KAMBING IRAN BERDASARKAN SEKUENS GEN CYTOCHROME OXIDASE I (COXI))

Mitochondrial DNA has been one of the most widely used molecular markers for phylogenetic studies in animals because of its simple genomic structure. This study examines the genetic characteristic of domestic goat using sequence analysis of mitochondrial DNA Cytochrome oxidase subunit I (COXI) to identify and differentiate among three common breeds (Adani, Najdi and Markhoz) of Iran. The genomic DNA was isolated by salting out method and amplified cytochrome oxidase I gene using Polymerase Chain Reaction (PCR) method with a pair of primer. Phylogenetic trees and pairwise calculations were obtained by using Mega 6 software. A partial sequence of cytochrome oxidase I gene of Iranian goats is 1286 bp and contained four variable sites and three haplotypes. Phylogenetic analysis of haplotype in the combination with the goat from GenBank showed that Iranian goatclustered in a separate lineage. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country