Identification of a Bacteria-produced Benzisoxazole with Antibiotic Activity against Multi-drug Resistant Acinetobacter baumannii

The emergence of multi-drug resistant pathogenic bacteria represents a serious and growing threat to national healthcare systems. Most pressing is an immediate need for the development of novel antibacterial agents to treat Gram-negative multi-drug resistant infections, including the opportunistic, hospital-derived pathogen, Acinetobacter baumannii. Herein we report a naturally occurring 1,2-benzisoxazole with minimum inhibitory concentrations as low as 6.25 μg ml−1 against clinical strains of multi-drug resistant A. baumannii and investigate its possible mechanisms of action. This molecule represents a new chemotype for antibacterial agents against A. baumannii and is easily accessed in two steps via de novo synthesis. In vitro testing of structural analogs suggest that the natural compound may already be optimized for activity against this pathogen. Our results demonstrate that supplementation of 4-hydroxybenzoate in minimal media was able to reverse 1,2-benzisoxazole’s antibacterial effects in A. baumannii. A search of metabolic pathways involving 4-hydroxybenzoate coupled with molecular modeling studies implicates two enzymes, chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyltransferase, as promising leads for the target of 3,6-dihydroxy-1,2-benzisoxazole

Some pulse characteristics of atmospheric radio noise bursts at 3 MHz

An atmospheric radio noise burst represents the radiation received from one complete lightning flash at the frequency to which a receiver is tuned and within the receiver bandwidth. At tropical latitudes, the principal source of interference in the frequency range from 0.1 to 10 MHz is the burst form of atmospheric radio noise. The structure of a burst shows several approximately rectangular pulses of random amplitude, duration and frequency of recurrence. The influence of the noise on data communication can only be examined when the value of the number of pulses crossing a certain amplitude threshold per unit time of the noise burst is known. A pulse rate counter designed for this purpose has been used at Bangalore (12°58′N, 77°35′E) to investigate the pulse characteristics of noise bursts at 3 MHz with a receiver bandwidth of 3.3 kHz/6d B. The results show that the number of pulses lying in the amplitude range between peak and quasi-peak values of the noise bursts and the burst duration corresponding to these pulses follow log normal distributions. The pulse rates deduced therefrom show certain correlation between the number of pulses and the duration of the noise burst. The results are discussed with a view to furnish necessary information for data communication

Implementation of a modified CVSD coder

This paper describes the implementation of the modified continuously variable slope delta modulator (MCVSD), in which the basic step size δ0 is made to vary as a function of input signal level. The information needed to carry out this is extracted at the local decoder output so that the coder and the decoder track each other. The result is a significant improvement in the dynamic range (about 15dB) as compared to CVSD coder without degrading the peak signal to noise ratio

Constant factor incremental delta modulator

This paper presents a new algorithm for the step-size change of instantaneous adaptive delta modulator. The present strategy is such that the step-size at any sampling instant can increase or decrease by either of the two constant factors or can remain the same, depending upon the combination of three or four most recent output bits. The quantizer has been simulated on a digital computer, and its performance compared with other quantizers. The figure of merit used is the SNR with gaussian signals as the input. The results indicate that the new design can give an improved SNR over a wider dynamic range and fast response to step inputs, as compared to the earlier systems

Ternary adder and subtracter using ternary multiplexer

This paper describes the use of a ternary multiplexer as a building block in the implementation of ternary adders and subtractors and also in the development of ternary coded adders/subtractors

Pipeline Analog To Ternary Converter

The paper describes the application of the pipelining principle to the realization of an analogue-to-ternary converter. The circuit shows a considerable saving in hard-ware compared with an earlier proposed circuit. The main hardware components used are analogue comparators, subtractors and the delay elements; hence this method of A/T conversion can operate at a higher sampling frequency

Low Voltage CMOS op-amp with Rail-to-Rail Input/Output Swing

As the supply voltage to a standard CMOS op-amp is reduced, the input common mode range and the output swing get reduced drastically. Special biasing circuits have to be used to raise them up to rail-to-rail supply voltage. Three low voltage op-amps with new biasing circuits have been proposed in this paper and their performance evaluated. The op-amp design is focused on dynamic range and high drive capability

A simple, wide-range, linear thermistor-based temperature indicator

A novel thermistor-based temperature indicator using an RC oscillator and an up/down counter has been developed and described. The indicator provides linear performance over a wide dynamic temperature range of 0-100°C. This indicator is free from the error due to lead resistances of the thermistor and gives a maximum error of ±0 · 1°C in the range 0-100°C. Test results are given to support the theory

Character recognition — A review

The machine replication of human reading has been the subject of intensive research for more than three decades. A large number of research papers and reports have already been published on this topic. Many commercial establishments have manufactured recognizers of varying capabilities. Handheld, desk-top, medium-size and large systems costing as high as half a million dollars are available, and are in use for various applications. However, the ultimate goal of developing a reading machine having the same reading capabilities of humans still remains unachieved. So, there still is a great gap between human reading and machine reading capabilities, and a great amount of further effort is required to narrow-down this gap, if not bridge it. This review is organized into six major sections covering a general overview (an introduction), applications of character recognition techniques, methodologies in character recognition, research work in character recognition, some practical OCRs and the conclusions

Quaternary unary operators

Unary operators are functions of a single variable. Realization of quaternary unary operators (QUOs) using quaternary multiplexer (QMUX) is presented in this paper. QUOs are divided into eight groups on the basis of the number of change overs in the output for an input sequence of 0, 1, 2, 3. This grouping reduces the hardware required to realize them. QMUX with two, three, and four input lines are proposed for the realization of QUOs belonging to the eight groups. A systematic procedure for the selection of QMUX and the implementation of the QUOs are given. The QMUXs are designed using CMOS ICs. The hardware required for their implementation is also discussed