Distribution and normality tests of five macronutrient and Na concentrations and K/Na ratio in leaves of 77 RILs.

<p>▼ = VH8; <b>▽</b> = GUA; ■ = Sipima 280, □ = Sicot 75; ◆ = population mean.</p

Nutrient, Na concentrations and K/Na ratio in different plant parts of two parents of the RIL population, Guazuncho 2 (GUA), <i>Gossypium hirsutum</i>, and VH8-4602 (VH8), <i>G</i>. <i>barbadense</i>.

<p>The pairs of bold values within the same plant tissue differed significantly between parents at P≤0.05.</p><p>Nutrient, Na concentrations and K/Na ratio in different plant parts of two parents of the RIL population, Guazuncho 2 (GUA), <i>Gossypium hirsutum</i>, and VH8-4602 (VH8), <i>G</i>. <i>barbadense</i>.</p

Estimates of narrow sense heritability (h²) and predicted selection response for five macro-, five micro-nutrient and Na concentrations and K/Na ratio on leaves of the RIL population under two selection intensities based on a combined data analysis over two seasons.

<p>Estimates of narrow sense heritability (h²) and predicted selection response for five macro-, five micro-nutrient and Na concentrations and K/Na ratio on leaves of the RIL population under two selection intensities based on a combined data analysis over two seasons.</p

Distribution and normality tests of five micronutrient concentration in leaves of 77 RILs.

<p>▼ = VH8; <b>▽</b> = GUA; ■ = Sipima 280, □ = Sicot 75; ◆ = population mean.</p

Description of QTLs affecting leaf macro-, micro-nutrient and Na concentrations and K/Na ratio in the RIL population of Guazuncho 2 × VH8-4602 as detected by composite interval mapping using QTLCartographer based on the estimates of a two year combined analysis.

<p>^a variables K and K/Na ratio were log-transformed before QTL analysis.</p><p>“Signif” column indicates significant QTLs (LOD superior to permutation-based threshold). Putative QTLs with LOD>2.5 are also indicated. Positive sign of additive effect indicate that parent GUA increases the trait. LOD2L-LOD2R and LOD1L-LOD1R are the positions in cM of the drop-off in 2 or 1 LOD units from the LOD peak, respectively. R² is the proportion of the trait variance explained by the QTL.</p

Pearson correlation coefficients among macro-, micro-nutrient and Na concentrations and K/Na ratio on leaves of the RIL population based on a combined analysis of two seasons.

<p>*,**,*** indicate significance at P≤0.05, 0.01, 0.001, respectively.</p><p>n = 77.</p><p>Pearson correlation coefficients among macro-, micro-nutrient and Na concentrations and K/Na ratio on leaves of the RIL population based on a combined analysis of two seasons.</p

Importance of variation source for five macro-, five micro-nutrient and Na concentrations and K/Na ratio on leaves revealed from a combined data analysis over two seasons.

<p>Importance of variation source for five macro-, five micro-nutrient and Na concentrations and K/Na ratio on leaves revealed from a combined data analysis over two seasons.</p

The percentage of autophagic vacuoles varies with <i>beclin-1</i> gene expression.

<p>The autophagic vacuoles was analyzed by calculating MDC-stained cardiomyocytes using flow cytometry. (<b>A, C</b> and <b>E</b>) Representative percentage of autophagic vacuoles measured in MDC-stained cardiomyocytes using flow cytometry. (<b>B</b>) Evaluation of the influence of 3-MA on autophagic vacuoles. Con: control group, Ang II: treated with 1 µmol/L Angiotensin II, and Ang II+3-MA: treated with 1 µmol/L Angiotensin II and 10 mmol/L 3-MA. *<i>P</i><0.05 compared with Con group; # <i>P</i><0.05 compared with Ang II group. (<b>D</b>) Evaluation of the influence of <i>beclin-1</i> specific siRNA on autophagic vacuoles. NC: treated with lentivirus containing negative control of <i>beclin-1</i>-specific siRNA, Ang II+ NC: treated with lentivirus containing negative control of <i>beclin-1</i>-specific siRNA and 1 µmol/L Angiotensin II, and Ang II+siRNA: treated with lentivirus containing <i>beclin-1</i>-specific siRNA and 1 µmol/L Angiotensin II. *<i>P</i><0.05 compared with NC group; # <i>P</i><0.05 compared with Ang II+NC group. (<b>F</b>) Evaluation of the influence of pRc/CMV2-beclin-1 vector on autophagic vacuoles. pRc/CMV2: transfected with pRc/CMV2 vector, and pRc/CMV2-beclin-1: transfected with pRc/CMV2-beclin-1. *<i>P</i><0.05 compared with pRc/CMV2 group. Data are presented as means ± SEM.</p

clinical characteristics of patients with and without LVH.

<p>DM = diabetes mellitus, ACS = acute coronary syndrome, Af = atrial fibrillation, SBP = systolic blood pressure, DBP = diastolic blood pressure, TC = total cholesterol, TG = total glyceride, HDL = high-density lipoprotein, LDL = low-density lipoprotein, Cr = creatinine, CK-MB = creatine kinase-MB, LV = left ventricular diameter.</p

Autophagy and miR-30 expression in rats after TAAC surgery.

<p>The relative expression of mRNA level, microRNA level, protein, and autophagic vacuoles in left ventricular tissues from Sham and TAAC rats was analyzed 4 weeks after the operation. (<b>A</b>) Relative mRNA expression level of <i>beclin-1</i> was analyzed by qRT-PCR. 18S was used as an internal control. (<b>B</b>) Relative expression of autophagy-related proteins, beclin-1 and LC3, was analyzed by Western blotting. GAPDH was used as an internal control. (<b>C</b>) Autophagic vacuoles (arrows indicated) were detected by transmission electron microscopy, at a magnification of ×13500. Scale bar: 1 µm. (<b>D</b>) Relative miR-30a, miR-30b and miR-30c expression which was presented as 2^−Δct*10⁻³ was analyzed by qRT-PCR. U6 was used as an internal control. Data are presented as means ± SEM. *<i>P</i><0.05 compared with Sham.</p