Diesel degradation capability and environmental robustness of strain Pseudomonas aeruginosa WS02

Petroleum hydrocarbon (PHC) degrading bacteria have been frequently discovered. However, in practical application, a single species of PHC degrading bacterium with weak competitiveness may face environmental pressure and competitive exclusion due to the interspecific competition between petroleum-degrading bacteria as well as indigenous microbiota in soil, leading to a reduced efficacy or even malfunction. In this study, the diesel degradation ability and environmental robustness of an endophytic strain Pseudomonas aeruginosa WS02, were investigated. The results show that the cell membrane surface of WS02 was highly hydrophobic, and the strain secreted glycolipid surfactants. Genetic analysis results revealed that WS02 contained multiple metabolic systems and PHC degradation-related genes, indicating that this strain theoretically possesses the capability of oxidizing both alkanes and aromatic hydrocarbons. Gene annotation also showed many targets which coded for heavy metal resistant and metal transporter proteins. The gene annotation-based inference was confirmed by the experimental results: GC-MS analysis revealed that short chain PHCs (C10–C14) were completely degraded, and the degradation of PHCs ranging from C15–C22 were above 90% after 14 d in diesel-exposed culture; Heavy metal (Mn2+, Pb2+ and Zn2+) exposure was found to affect the growth of WS02 to some extent, but not its ability to degrade diesel, and the degradation efficiency was still maintained at 39–59%. WS02 also showed a environmental robustness along with PHC-degradation performance in the co-culture system with other bacterial strains as well as in the co-cultured system with the indigenous microbiota in soil fluid extracted from a PHC-contaminated site. It can be concluded that the broad-spectrum diesel degradation efficacy and great environmental robustness give P. aeruginosa WS02 great potential for application in the remediation of PHC-contaminated soil.<br/

19th dec fish 8 hypoxia

continuous time series data for cardiac contraction recorded using World Precision Instruments software DataTra

18th dec fish 5 hypoxia

continuous time series data for cardiac contraction recorded using World Precision Instruments software DataTra

20th dec fish 10 hypoxia

20th dec fish 10 hypoxi

18th dec fish 4 hypoxia

continuous time series data for cardiac contraction recorded using World Precision Instruments software DataTra

raw contractile force data fish 1

Data is recorded as a continuous time series using World Precision Instruments Software DataTrax

17th dec fish 3 hypoxia

continuous time series data for cardiac contraction recorded using World Precision Instruments software DataTra

19th dec fish 7 hypoxia

continuous time series data for cardiac contraction recorded using World Precision Instruments software DataTra