İnvaziv duktal karsinom tanısı almış meme kanserli hastalarda, Ki-67 ile diğer prognostik faktörler arasındaki ilişki

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Meme kanseri hücre hattı MCF-7 üzerinde arsenik trioksit'in sitotoksisitesi ve diğer kemoterapötik ajanlarla etkileşimi

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Response Surface Methodology for Extraction of Curcumin from Turmeric and Piperine from Black Pepper

Considering that approximately a third of all drugs currently on the market is derived from natural products, curcumin attracted attention due to its anticancer, antioxidative, antiinflammatory and antimicrobial properties. Unfortunately, its low solubility and depending on that a poor bioavailability are limiting factors for its clinical application. It was shown that the administration of curcumin with piperin, the main component of black pepper, increased the bioavailability of curcumin. In addition, piperine increased the plasma concentration and delayed the elimination of drugs like phenytoin and rifampin, and has antiinflammatory and antifungal properties among others. Considering the literature has shown that for extracting curcumin and piperine, respectively, Soxhlet, microwave-assisted extraction, supercritical carbon dioxide extraction and concentional extraction with ethanol as solvent were used among others. According to the concentional extraction of curcumin with ethanol important parameter like the ethanol concentration was not investigated. In addition the maximum extraction time in this case was only 50 minutes, too short in our opinion. Due to these facts the optimum extraction parameters for the conventional extraction of curcumin from turmeric and of piperine from black pepper, respectively, were investigted in this study, with respect to extraction time of 7-21 hours, ethanol concentration of 10-90 % and drug to solvent ratio of 1:10-1:30. Response surface methodology was used as a tool to determine the optimum conditions for the extraction of curcumin and piperine, with help of an experimental design, central composite design. The ideal parameter for this conventional extraction of curcumin from turmeric and of piperine from black pepper, respectively, were an extraction time of 15 hours, an ethanol concentration of 70 % (v/v) and a drug to solvent ratio of 1:20.Considering that approximately a third of all drugs currently on the market is derived from natural products, curcumin attracted attention due to its anticancer, antioxidative, antiinflammatory and antimicrobial properties. Unfortunately, its low solubility and depending on that a poor bioavailability are limiting factors for its clinical application. It was shown that the administration of curcumin with piperin, the main component of black pepper, increased the bioavailability of curcumin. In addition, piperine increased the plasma concentration and delayed the elimination of drugs like phenytoin and rifampin, and has antiinflammatory and antifungal properties among others. Considering the literature has shown that for extracting curcumin and piperine, respectively, Soxhlet, microwave-assisted extraction, supercritical carbon dioxide extraction and concentional extraction with ethanol as solvent were used among others. According to the concentional extraction of curcumin with ethanol important parameter like the ethanol concentration was not investigated. In addition the maximum extraction time in this case was only 50 minutes, too short in our opinion. Due to these facts the optimum extraction parameters for the conventional extraction of curcumin from turmeric and of piperine from black pepper, respectively, were investigted in this study, with respect to extraction time of 7-21 hours, ethanol concentration of 10-90 % and drug to solvent ratio of 1:10-1:30. Response surface methodology was used as a tool to determine the optimum conditions for the extraction of curcumin and piperine, with help of an experimental design, central composite design. The ideal parameter for this conventional extraction of curcumin from turmeric and of piperine from black pepper, respectively, were an extraction time of 15 hours, an ethanol concentration of 70 % (v/v) and a drug to solvent ratio of 1:20

Curcumin and piperine loaded zein-chitosan nanoparticles: Development and in-vitro characterisation

WOS: 000427445500004PubMed ID: 29556123Curcumin as the active compound of turmeric has antioxidative, antiinflammatory, antimicrobial and anticancer properties among others. However, its disadvantageous properties like low solubility, poor bioavailability and rapid degradation under neutral or alkaline pH conditions or when exposed to light limit its clinical application. These problems can be solved by a smart combination of using a natural enhancer like piperine and preparing nanoparticles by a proper method like electrospray. Due to these facts it was aimed in this study to develop curcumin and piperine loaded zein-chitosan nanoparticles step by step. For that purpose various formulation parameters like the concentrations of zein, curcumin, piperine and chitosan and the preparation parameters like the applied voltage and the nozzle diameter were investigated step by step. The nanoparticles were characterised by investigating their shapes, morphologies, particle sizes with help of SEM images and the cytotoxicity on neuroblastoma cells. It was succeeded to prepare curcumin and piperine loaded zein-chitosan nanoparticles having a mean particle size of approximately 500 nm and high encapsulation efficencies for curcumin (89%) and piperine (87%). Using a curcumin concentration of 10-25 mg/ml resulted in reduction of the viability of approximately 50% of the neuroblastoma cells. The here developed nanoparticle formulation consisting of solely natural compounds showed good cytotoxic effects and is a promising approach with appropriate properties for final consumption. (C) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University