90 research outputs found
Comprehensive transcriptome analysis of fluid shear stress altered gene expression in renal epithelial cells
Isolation of primary avian osteocytes
Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3
Rapid release of nitric oxide in response to mechanical stress by bone cells grown from adult mouse long bone fragments
Osteocytes subjected to pulsating fluid flow regulate osteoblast proliferation and differentiation
Osteocytes subjected to pulsating fluid flow regulate osteoblast proliferation and differentiation
Different response of cells from adult and neonatal mouse bone to biochemical and mechanical challenge
Rapid release of nitric oxide in response to mechanical stress by bone cells grown from adult mouse long bone fragments
Low-intensity pulsed ultrasound affects human articular chondrocytes in vitro
We investigated whether low-intensity pulsed ultrasound (LIPUS) stimulates chondrocyte proliferation and matrix production in explants of human articular cartilage obtained from donors suffering from unicompartimental osteoarthritis of the knee, as well as in isolated human chondrocytes in vitro. Chondrocytes and explants were exposed to LIPUS (30 mW/cm2; 20 min/day, 6 days). Stimulation of [35S]-sulphate incorporation into proteoglycans by LIPUS was 1.3-fold higher in degenerative than in collateral monolayers as assessed biochemically and 1.9-fold higher in explants as assessed by autoradiography. LIPUS decreased the number of cell nests containing 1-3 chondrocytes by 1.5 fold in collateral and by 1.6 fold in degenerative explants. LIPUS increased the number of nests containing 4-6 chondrocytes by 4.8 fold in collateral and by 3.9 fold in degenerative explants. This suggests that LIPUS stimulates chondrocyte proliferation and matrix production in chondrocytes of human articular cartilage in vitro. LIPUS might provide a feasible tool for cartilage tissue repair in osteoarthritic patients, since it stimulates chondrocyte proliferation and matrix production
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