Keck Spectroscopy of Two Young Globular Clusters in the Merger Remnant NGC 3921

Low-resolution UV-to-visual spectra of two candidate globular clusters in the merger remnant NGC 3921 are presented. These two clusters of apparent magnitude V = 22.2 (Mv = -12.5) lie at projected distances of ~5 kpc from the center and move with halo-type radial velocities relative to the local galaxy background. Their spectra show strong Balmer absorption lines indicative of main-sequence turnoffs dominated by A-type stars. Comparisons with model-cluster spectra computed by Bruzual & Charlot and others yield cluster ages in the range of 200-530 Myr, and metallicities about solar to within a factor of three. Given their small half-light radii (Reff < 5 pc) and ages corresponding to ~100 core- crossing times, these clusters are gravitationally bound and, hence, indeed young globulars. Assuming that they had Chabrier-type initial mass functions, their estimated current masses are 2.3(+-0.1)x10^6 Msun and 1.5(+-0.1)x10^6 Msun, respectively, or roughly half the mass of omegaCen. Since NGC 3921 itself shows many signs of being a 0.7(+-0.3) Gyr old protoelliptical, these two young globulars of roughly solar metallicity and their many counterparts observed with the Hubble Space Telescope provide supporting evidence that, in the process of forming elliptical-like remnants, major mergers of gas-rich disks can also increase the number of metal-rich globular clusters. (Abridged)Comment: 22 pages, 6 figures, accepted for publication in AJ, July 200

A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs

<p>Abstract</p> <p>Background</p> <p>To find candidate genes that potentially influence the susceptibility or resistance of crop plants to powdery mildew fungi, an assay system based on transient-induced gene silencing (TIGS) as well as transient over-expression in single epidermal cells of barley has been developed. However, this system relies on quantitative microscopic analysis of the barley/powdery mildew interaction and will only become a high-throughput tool of phenomics upon automation of the most time-consuming steps.</p> <p>Results</p> <p>We have developed a high-throughput screening system based on a motorized microscope which evaluates the specimens fully automatically. A large-scale double-blind verification of the system showed an excellent agreement of manual and automated analysis and proved the system to work dependably. Furthermore, in a series of bombardment experiments an RNAi construct targeting the <it>Mlo </it>gene was included, which is expected to phenocopy resistance mediated by recessive loss-of-function alleles such as <it>mlo5</it>. In most cases, the automated analysis system recorded a shift towards resistance upon RNAi of <it>Mlo</it>, thus providing proof of concept for its usefulness in detecting gene-target effects.</p> <p>Conclusion</p> <p>Besides saving labor and enabling a screening of thousands of candidate genes, this system offers continuous operation of expensive laboratory equipment and provides a less subjective analysis as well as a complete and enduring documentation of the experimental raw data in terms of digital images. In general, it proves the concept of enabling available microscope hardware to handle challenging screening tasks fully automatically.</p

The accumulation of plutonium isotopes (239+240) in the components of the natural environment in the territory of the Karaganda region adjacent to the Semipalatinsk test site

Presents the results of a radiological survey of the territory of the Karaganda region. The data obtained on contamination of isotopes of plutonium (239+240), assessed the impact of nuclear testing on Semipalatinsk testing nuclear polygon. Currently, the pollution of the examined areas of technogenic radionuclides in most places, does not exceed the background level of global fallout, and only at some points noted in the reading background of global fallout. However, there are local areas of contamination, which could be formed as a result of testing of BRV, or other atmospheric fallout from nuclear testing

A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs

Background To find candidate genes that potentially influence the susceptibility or resistance of crop plants to powdery mildew fungi, an assay system based on transient-induced gene silencing (TIGS) as well as transient over-expression in single epidermal cells of barley has been developed. However, this system relies on quantitative microscopic analysis of the barley/powdery mildew interaction and will only become a high-throughput tool of phenomics upon automation of the most time-consuming steps. Results We have developed a high-throughput screening system based on a motorized microscope which evaluates the specimens fully automatically. A large-scale double-blind verification of the system showed an excellent agreement of manual and automated analysis and proved the system to work dependably. Furthermore, in a series of bombardment experiments an RNAi construct targeting the Mlo gene was included, which is expected to phenocopy resistance mediated by recessive loss-of-function alleles such as mlo5. In most cases, the automated analysis system recorded a shift towards resistance upon RNAi of Mlo, thus providing proof of concept for its usefulness in detecting gene-target effects. Conclusion Besides saving labor and enabling a screening of thousands of candidate genes, this system offers continuous operation of expensive laboratory equipment and provides a less subjective analysis as well as a complete and enduring documentation of the experimental raw data in terms of digital images. In general, it proves the concept of enabling available microscope hardware to handle challenging screening tasks fully automatically

An adaptive multiscale finite element method

This work is devoted to an adaptive multiscale finite element method (MsFEM) for solving elliptic problems with rapidly oscillating coeefficients. Starting from a general version of the MsFEM with oversampling, we de- rive an a posteriori estimate for the H1-error between the exact solution of the problem and a corresponding MsFEM approximation. Our esti- mate holds without any assumptions on scale separation or on the type of the heterogeneity. The estimator splits into different contributions which account for the coarse grid error, the fine grid error and the oversampling error. Based on the error estimate we construct an adaptive algorithm that is validated in numerical experiments

Ages and Metallicities of Young Globular Clusters in the Merger Remnant NGC 7252

UV-to-visual spectra of eight young star clusters in the merger remnant and protoelliptical galaxy NGC 7252, obtained with the Blanco 4-m telescope on Cerro Tololo, are presented. These clusters lie at projected distances of 3-15 kpc from the center and move with a velocity dispersion of 140+/-35 km/s in the line of sight. Seven of the clusters show strong Balmer absorption lines in their spectra [EW(H-beta)= 6-13 Angstrom], while the eighth lies in a giant HII region and shows no detectable absorption features. Based on comparisons with model-cluster spectra by Bruzual & Charlot (1996) and Bressan, Chiosi, & Tantalo (1996), six of the absorption-line clusters have ages in the range of 400-600 Myr, indicating that they formed early on during the recent merger. These clusters are globular clusters as judged by their small effective radii and ages corresponding to ~100 core crossing times. The one emission-line object is <10 Myr old and may be a nascent globular cluster or an OB association. The mean metallicities measured for three clusters are solar to within +/-0.15 dex, suggesting that the merger of two likely Sc galaxies in NGC 7252 formed a globular-cluster system with a bimodal metallicity distribution. Since NGC 7252 itself shows the characteristics of a 0.5-1 Gyr old protoelliptical, its second-generation solar-metallicity globulars provide direct evidence that giant ellipticals with bimodal globular-cluster systems can form through major mergers of gas-rich disk galaxies.Comment: 34 pages, incl. 6 figures in EPS format, AAS LaTeX, to be published in AJ, Vol. 116, Nov. 199