Yeast system as a screening tool for pharmacological assessment of G protein coupled receptors

International audienc

Modulation des réponses aux odorants de la muqueuse olfactive de rat par les peptides métaboliques : focus sur le NPY

National audienc

Modulation de la perception olfactive par l'endothéline

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Short-term effects of endothelin in rat olfactory mucosa : modulation of odorant detection and intercellular communication

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Endothelin as a neuroprotective factor in the olfactory epithelium

International audienceIn mammals, the olfactory sensory neurons are the only ones directly in contact with an aggressive environment. Thus, the olfactory mucosa is one of the few neuronal zones which are continuously renewed during adulthood. We have previously shown that endothelin is locally matured in the olfactory mucosa and that olfactory sensory neurons preferentially express ET(B) receptors, while ET(A) receptors are rather present in non neuronal olfactory mucosa cells. In addition to its vasoactive effect, the endothelin system is known for its pleiotropic effects including the modulation of cell population dynamics. We thus examined its potential neuroprotective effect in the olfactory mucosa using a primary culture of olfactory sensory neurons lying on non neuronal cells. While a serum deprivation led to a massive decrease of the density of olfactory sensory neurons in the primary cultures, endothelin 1 (ET-1) rescued part of the neuronal population through both ET(A) and ET(B) receptors. This effect was mainly anti-apoptotic as it reduced cleaved caspase-3 signal and nuclear condensation. Furthermore, the olfactory epithelium of ET(B)-deficient rats displayed increased apoptosis. These results strongly suggest that ET-1 acts as an anti-apoptotic factor on olfactory sensory neurons, directly through ET(B) and indirectly by limiting non neuronal cells death through ET(A)

Endothelin modulates both short-term kinetics of odorant detection and long-term cellular population dynamics in olfactory mucosa

International audienc

Coherence and Stochastic Dominance

National audienc

Endothelin modulates both short-term detection kinetics of odors and long-term cellular population dynamics in olfactory mucosa

International audienc