Pengaruh Radiasi Sinar Gamma Terhadap Kultur in Vitro Tanaman Jahe

In vitro shoot explants of white ginger and red ginger are irradiated by two different techniques. The first, the tuber explants are irradiated by 2 levels of gamma rays namely 10 and 30 Gy. The second, the shoot explants are irradiated by 2 levels of gamma rays namely 7,5 and 12,5 Gy. The irradiated explants are regenerated on modified MS medium with BAP 2 ppm and NAA 0,25 ppm. The result show that the irradiated tuber explants with 10 and 30 Gy could not initiate new regenerant of ginger. On the other hand the irradiated shoot explants with 7,5 and 12,5 Gy have been able to influence the growth and development of shoot, leaf and root. It is indicated that the level of gamma rays irradiation on shoot explants influences the induced putative mutant of ginger. However, the sprouting of red ginger on 12,5 Gy is higher and faster than 7,5 Gy, and white ginger as well as control. The other morphological characters have not been able to be identified, the experiment are still in process to detect the mutagenic influences

Pengaruh BAP dan Sukrosa terhadap Perbanyakan Jahe Empirit (Zingiber Officinale Rosc Var. Amarun) secara In Vitro

The need to produce numerous and good quality plantlet in short time has been carried out with in vitro culture. The objective of this research was to study BAP and sucrose effect on the in vitro multiplication of small ginger. Research was done in Plant Tissue Culture Laboratory, Department of Agronomy. Bogor Agricultural University from November 2002 until August 2003. The treatment used BAP 0, 1, 2, 3, 4, 5 ppm and sucrose 20, 30, 40, 50 g/l. The result showed that sucrose significantly influenced shoot number in 2-5 and 8 Week After Planting (WAP) and leaf number in 2-7 WAP; root length, root number and explant fresh weight. BAP only significantly influenced leaf number in 7 WAP and root quality. With time shoot color changed from green to yellow. Higher RAP and sucrose concentration increased micro rhizome percentage. Sucrose 40 g/l or RAP 2 ppm gave numerous shoot and high explant fresh weight