Phyto-pharmacology of Momordica dioica Roxb. ex. Willd: A Review

Momordica dioica Roxb. ex. Willd (Cucurbitaceae ) is commonly known as a biiter gourd. It is traditionally used as astringent, febrifuge, antiseptic, anthelmintic, spermicidal. Also Used in bleeding piles, urinary infection and as a sedative. Studies indicate that it possesses antioxidant, hepatoprotective, antibacterial, anti-inflammatory, anti-lipid peroxidative, hypoglycemic and analgesic properties.This review aims to highlight the ethnobotany, pharmacognostic and pharmacological uses of Momordica dioicaKeywords: Momordica dioica, Cucurbitaceae, phytochemical constituents, pharmacological actions, Parora , kakor

Geoinformatics-based investigation of slope failure and landslide damming of Chenab River, Lahaul-Spiti, Himachal Pradesh, India

A huge landslide blocked the flow of the Chenab River near Nalda village on the morning of 13th August 2021 in the Lahaul-Spiti district of Himachal Pradesh, which led to the flooding of several villages (Nalda, Jasrath, Tarang, etc.) in the Udaipur subdivision. The slope (∼200 ​m height, ∼220 ​m width, and approx. 30 ​m depth) on the left bank of the Chenab River failed, which brought colossal soil and debris. This resulted in the damming of the Chenab River near Leh Baring village, which lies upstream of Nalda Village Bridge and opposite to Junde village, creating a huge water reservoir that later started overflowing, posing a major threat to downstream villages. This caused damage to the houses located downstream, which were submerged, animals were also washed away, and a large part of agricultural land was also inundated. The event was observed and studied using satellite images of high-resolution obtained from Google Earth and Sentinel 2A. The Spatio-temporal satellite images were used to observe the scars developed in the region over the past few years along the river, which evidently show the early signatures of slope failure. To analyze the stability of the hill slope, the factor of safety of the hill was evaluated using SLOPE/W GeoStudio software. Slope map and drainage density map were also generated, showing the vulnerability of the hill slope. The debris flow runout simulation of the event was performed using the RAMMS debris flow model to calculate the volume of the landslide and the water reservoir formed due to damming of the Chenab River. The volume of the landslide (debris) due to slope failure was approx. 2.22 million m3 and the water reservoir volume was approximately 0.3 million m3. The significant factors accountable for the landslide i.e. the slope of the hill, geomorphology of the area, lithology (phyllites), presence of narrow and elongated valleys, the confluences of the river in the area, and continuous rainfall in the region during that period were evaluated. The paper also emphasizes monitoring of such vulnerable areas based on high-resolution time series satellite images, which are available on a regular basis to avoid the loss of human lives in the future

Interlaboratory evaluation of endotoxin analyses in agricultural dustscomparison of lal assay and mass spectrometry

Endotoxin exposure is associated with wheeze and asthma morbidity, while early life exposure may reduce risk of allergy and asthma. Unfortunately, it is difficult to compare endotoxin results from different laboratories and environments. We undertook this study to determine if lipopolysaccharide (LPS) extraction efficiency could account for differences among laboratories. We generated and collected aerosols from chicken and swine barns, and corn processing. We randomly allocated side-by-side filter samples to five laboratories for Limulus assay of endotoxin. Lyophilized aliquots of filter extracts were analyzed for 3-hydroxy fatty acids (3-OHFAs) as a marker of LPS using gas chromatography-mass spectrometry. There were significant differences in endotoxin assay and GC-MS (LPS) results between laboratories for all dust types (p < 0.01). Patterns of differences between labs varied by dust type. Relationships between assay and GC/MS results also depended on dust type. The percentages of individual 3-OHFA chain lengths varied across labs (p < 0.0001) suggesting that each lab recovered a different fraction of the LPS available. The presence of large amounts of particle associated LPS and absence of a freezing thawing cycle were associated with lower correlations between LPS and bioactivity, consistent with an absence of Limulus response to cell-bound endotoxin. These data suggest that extraction methods affect endotoxin measurements. The LAL methods may be most suitable when comparing exposures within similar environments; GC-MS offers additional information helpful in optimizing sample treatment and extraction. GC-MS may be of use when comparing across heterogeneous environments and should be considered for inclusion in future studies of human health outcomes

Interlaboratory evaluation of endotoxin analyses in agricultural dusts - comparison of LAL assay and mass spectrometry

Endotoxin exposure is associated with wheeze and asthma morbidity, while early life exposure may reduce risk of allergy and asthma. Unfortunately, it is difficult to compare endotoxin results from different laboratories and environments. We undertook this study to determine if lipopolysaccharide ( LPS) extraction efficiency could account for differences among laboratories. We generated and collected aerosols from chicken and swine barns, and corn processing. We randomly allocated side-by-side filter samples to five laboratories for Limulus assay of endotoxin. Lyophilized aliquots of filter extracts were analyzed for 3-hydroxy fatty acids ( 3-OHFAs) as a marker of LPS using gas chromatography-mass spectrometry. There were significant differences in endotoxin assay and GC-MS ( LPS) results between laboratories for all dust types ( p < 0.01). Patterns of differences between labs varied by dust type. Relationships between assay and GC/MS results also depended on dust type. The percentages of individual 3-OHFA chain lengths varied across labs ( p < 0.0001) suggesting that each lab recovered a different fraction of the LPS available. The presence of large amounts of particle associated LPS and absence of a freezing thawing cycle were associated with lower correlations between LPS and bioactivity, consistent with an absence of Limulus response to cell-bound endotoxin. These data suggest that extraction methods affect endotoxin measurements. The LAL methods may be most suitable when comparing exposures within similar environments; GC-MS offers additional information helpful in optimizing sample treatment and extraction. GC-MS may be of use when comparing across heterogeneous environments and should be considered for inclusion in future studies of human health outcomes