Isometric handgrip strength of agricultural workers from northeast region of India

Handgrip strength is considered as one of the most important factors for performing various agricultural tasks related to torqueing, lifting, pulling, pushing, etc. Hand tools and equipment which are designed based on anthropometric/strength data of different population, may not be suitable for any particular targeted user group. As a result work related musculoskeletal disorders (WMSDs) at upper extremity may occur very often. Lack of strength data of Assamese population (people of Assam, a state in northeast India), motivated present authors to conduct a survey on isometric handgrip strength data of 200 agricultural workers (130 male and 70 female, aged 17-62 years) from the Kamrup district of the state. Maximal isometric handgrip strength was determined using a handheld handgrip dynamometer with standard testing position, protocol and instructions. Descriptive statistics such as mean, standard deviation (SD), percentiles etc. were summarized. Results of student’s t-tests showed significant differences (

Isometric muscular strength data of Indian agricultural workers for equipment design: Critical analysis

Abstract: Human muscular strength is extensively used in Indian agriculture for operating various push-pull type farm tools and equipment.  Incompatibility between operators’ physical capabilities (anthropometric and biomechanical) and demands of physical task to operate tools/equipment often leads to poor performance, low productivity and safety problems.  Although anthropometric data are generally being considered, an inadvertent negligence of using strength database for agricultural tools/equipment design is very common in developing countries like India.  Therefore, in present paper an attempt has been made to statistically analyze available strength data (pooled and regional/state wise data) of male and female Indian agricultural workers to understand nature of variability of those data in terms of difference between pooled Indian data vs. individual state data; difference between male vs. female data across various states of India; and for determining safe operational force limits for handling various agricultural tools/equipment.  Critical evaluation of male and female strength data revealed that there are significant differences (p<0.01 or p<0.05) between mean values of pooled Indian data vs. individual state data for almost all strength variables under study.  It has also been observed that average muscular strength of female is significantly lower (in general 2/3rd of male) than their male counter parts across all states.  Thus, present study concluded that regional variations and gender variation of isometric strength data are crucial ergonomic consideration for using percentile strength data during calculation of operational force limits for designing various agricultural tools/equipment to be used by targeted user populations from various parts of a country like India with huge ethnic diversity.   Keywords: ergonomics, muscular strength, tools and equipment, agricultural workers, Indi

Normal and pathogenic variation of RFC1 repeat expansions: implications for clinical diagnosis

Cerebellar Ataxia, Neuropathy and Vestibular Areflexia Syndrome (CANVAS) is an autosomal recessive neurodegenerative disease, usually caused by biallelic AAGGG repeat expansions in RFC1. In this study, we leveraged whole genome sequencing (WGS) data from nearly 10,000 individuals recruited within the Genomics England sequencing project to investigate the normal and pathogenic variation of the RFC1 repeat. We identified three novel repeat motifs, AGGGC (n=6 from 5 families), AAGGC (n=2 from 1 family), AGAGG (n=1), associated with CANVAS in the homozygous or compound heterozygous state with the common pathogenic AAGGG expansion. While AAAAG, AAAGGG and AAGAG expansions appear to be benign, here we show a pathogenic role for large AAAGG repeat configuration expansions (n=5). Long read sequencing was used to fully characterise the entire repeat sequence and revealed a pure AGGGC expansion in six patients, whereas the other patients presented complex motifs with AAGGG or AAAGG interruptions. All pathogenic motifs seem to have arisen from a common haplotype and are predicted to form highly stable G quadruplexes, which have been previously demonstrated to affect gene transcription in other conditions. The assessment of these novel configurations is warranted in CANVAS patients with negative or inconclusive genetic testing. Particular attention should be paid to carriers of compound AAGGG/AAAGG expansions, since the AAAGG motif when very large (>500 repeats) or in the presence of AAGGG interruptions. Accurate sizing and full sequencing of the satellite repeat with long read is recommended in clinically selected cases, in order to achieve an accurate molecular diagnosis and counsel patients and their families