クロロゲン酸類のDPPHラジカル消去活性とマウスマクロファージ様細胞に対する影響について

Green coffee contains a large amount of polyphenols, especially caffeoyl quinic acid (CQA) related compounds. Using TOYOPEARL HW-40F column chromatography, five fractions (I～V) were separated from green coffee bean extract. HPLC technique demonstrated that Frs. II and III were mainly composed of CQA, whereas Frs. IV and V were mainly cmposed of diCQA. We measured the DPPH radical scavenging activity of these fractions. Frs. III and V had higher DPPH radical scavenging activities than green coffee bean extract. We also investigated the effect of these fractions on the cytotoxicity and the nitric oxide (NO) production by LPS-activated mouse macrophage-like Raw 264.7 cells. Noncytotoxic concentration of Fr. III (CQA fraction) inhibited NO production by activated Raw 264.7 cells. Fr. IV (diCQA fraction) showed higher cytotoxicity and increased NO production at lower concentration. The present study demonstrated that green coffee bean extract contains numerous substance which regulate the function of activated macrophages

The Japanese space gravitational wave antenna; DECIGO

DECi-hertz Interferometer Gravitational wave Observatory (DECIGO) is the future Japanese space gravitational wave antenna. DECIGO is expected to open a new window of observation for gravitational wave astronomy especially between 0.1 Hz and 10 Hz, revealing various mysteries of the universe such as dark energy, formation mechanism of supermassive black holes, and inflation of the universe. The pre-conceptual design of DECIGO consists of three drag-free spacecraft, whose relative displacements are measured by a differential Fabry– Perot Michelson interferometer. We plan to launch two missions, DECIGO pathfinder and pre- DECIGO first and finally DECIGO in 2024

DECIGO pathfinder

DECIGO pathfinder (DPF) is a milestone satellite mission for DECIGO (DECi-hertz Interferometer Gravitational wave Observatory) which is a future space gravitational wave antenna. DECIGO is expected to provide us fruitful insights into the universe, in particular about dark energy, a formation mechanism of supermassive black holes, and the inflation of the universe. Since DECIGO will be an extremely large mission which will formed by three drag-free spacecraft with 1000m separation, it is significant to gain the technical feasibility of DECIGO before its planned launch in 2024. Thus, we are planning to launch two milestone missions: DPF and pre-DECIGO. The conceptual design and current status of the first milestone mission, DPF, are reviewed in this article

フキノール酸のラジカル消去能について

今まで報告例のないフキノール酸(FA)のラジカル消去能について、クロロゲン酸(CGA)、エピガロカテキンガレート(EGCG)、没食子酸(GA)と比較して検討した。スーパーオキシドアニオン(HX-XOD反応で生成)、ヒドロキシルラジカル(フェントン反応で生成)、一酸化窒素(NOC-7より生成)およびDPPHラジカル消去能はESR法を用いて測定した。またDPPHラジカル消去能は吸光度法でも測定した。その結果、スーパーオキシドアニオン消去能はEGCG>GA>FA>CGAの順、ヒドロキシルラジカル消去能はEGCG>CGA>FA>GAの順、一酸化窒素消去能はEGCG>FA>CGAの順、ESR法で測定したDPPHラジカル消去能はEGCG>GA>FA>CGAの順、そして吸光度法で測定したDPPHラジカル消去能はEGCG>FA>CGAの順となった。本研究によりFAは抗酸化剤としての利用が期待できると考えられる。Radical scavenging activity of fukinolic acid (FA), chlorogenic acid (CGA), epigallocatechin gallate (EGCG) and gallic acid (GA) against superoxide anion (produced by HX-XOD reaction), hydroxyl radical (produced by Fenton reaction), nitric oxide (NO) (produced by NOC-7 with carboxy-PTIO) and DPPH radical was measured by ESR spectroscopy and OD method. The radical scavenging activity of these compounds was in the following order : (1) superoxide anion, EGCG>GA>FA>CGA, (2) hydroxyl radical, EGCG>CGA>FA>GA, (3) NO, EGCG>FA>CGA, and (4) DPPH radical, EGCG>GA>FA>CGA (ESR method), EGCG>FA>CGA (OD method). These data suggest that FA should be considered as a candidate of applicable antioxidant as well as other polyphenols

The status of DECIGO

DECIGO (DECi-hertz Interferometer Gravitational wave Observatory) is the planned Japanese space gravitational wave antenna, aiming to detect gravitational waves from astrophysically and cosmologically significant sources mainly between 0.1 Hz and 10 Hz and thus to open a new window for gravitational wave astronomy and for the universe. DECIGO will consists of three drag-free spacecraft arranged in an equilateral triangle with 1000 km arm lengths whose relative displacements are measured by a differential Fabry-Perot interferometer, and four units of triangular Fabry-Perot interferometers are arranged on heliocentric orbit around the sun. DECIGO is vary ambitious mission, we plan to launch DECIGO in era of 2030s after precursor satellite mission, B-DECIGO. B-DECIGO is essentially smaller version of DECIGO: B-DECIGO consists of three spacecraft arranged in an triangle with 100 km arm lengths orbiting 2000 km above the surface of the earth. It is hoped that the launch date will be late 2020s for the present