The association between the expression of the HER family members and disease-free survival in univariate and multivariate analysis.

<p>P-value of <i>≤0.05</i> was considered significant.</p

The association between EGFR and EGFR/HER-4 and disease-free survival in Dukes’ C and D colon cancer patients.

<p>Kaplan-Meier survival curves showing the impact on the disease-free survival of the patients with EGFR expression (A), membranous EGFR expression (B), EGFR 1+ immunostaining (C) and EGFR/HER-4 co-expression (D). A log-rank test value of <i>P≤0.05</i> was considered statistical significance.</p

Immunohistochemical expression of HER family members in Dukes’ C and D colon cancer patients.

<p>Immunohistochemical expression of HER family members in Dukes’ C and D colon cancer patients.</p

The immunostaining of HER family members in Dukes’ C and D colon cancer specimens.

<p>Immunostaining of EGFR 3+, HER-2 3+, HER-3 2+, and HER-4 3+ in formalin fixed paraffin embedded tumour sections stained immunohistochemically, as described under methods and patients section. Magnification: ×200.</p

Co-expression level of HER family members presented as percentage positive tumour cells immunostaining in Dukes’ C and D colon cancer patients.

<p>Co-expression level of HER family members presented as percentage positive tumour cells immunostaining in Dukes’ C and D colon cancer patients.</p

The co-expression of all HER family members in a patient with Dukes’ C colon cancer.

<p>Co-expression of EGFR, HER-2, HER-3, and HER-4 in a formalin fixed paraffin embedded tumour sections stained immunohistochemically as described under methods and patients section. Magnification: ×200.</p

Clinicopathological parameters and survival of Dukes’ C and D colon cancer patients.

<p>Overall survival and disease-free survival relative to the indicated features was determined by Kaplan-Meier analysis and the log-rank test. P-value of <i>≤0.05</i> was considered significant.</p

The association between HER family expression and clinicopathological characteristics using the Chi-squared test (Fisher’s exact test, FET).

<p>P-value of <i>≤0.05</i> was considered significant.</p