Mindcraft, a mobile mental health monitoring platform for children and young people: development and acceptability pilot study

BACKGROUND: Children and young people's mental health is a growing public health concern, which is further exacerbated by the COVID-19 pandemic. Mobile health apps, particularly those using passive smartphone sensor data, present an opportunity to address this issue and support mental well-being. OBJECTIVE: This study aimed to develop and evaluate a mobile mental health platform for children and young people, Mindcraft, which integrates passive sensor data monitoring with active self-reported updates through an engaging user interface to monitor their well-being. METHODS: A user-centered design approach was used to develop Mindcraft, incorporating feedback from potential users. User acceptance testing was conducted with a group of 8 young people aged 15-17 years, followed by a pilot test with 39 secondary school students aged 14-18 years, which was conducted for a 2-week period. RESULTS: Mindcraft showed encouraging user engagement and retention. Users reported that they found the app to be a friendly tool helping them to increase their emotional awareness and gain a better understanding of themselves. Over 90% of users (36/39, 92.5%) answered all active data questions on the days they used the app. Passive data collection facilitated the gathering of a broader range of well-being metrics over time, with minimal user intervention. CONCLUSIONS: The Mindcraft app has shown promising results in monitoring mental health symptoms and promoting user engagement among children and young people during its development and initial testing. The app's user-centered design, the focus on privacy and transparency, and a combination of active and passive data collection strategies have all contributed to its efficacy and receptiveness among the target demographic. By continuing to refine and expand the app, the Mindcraft platform has the potential to contribute meaningfully to the field of mental health care for young people

Estimativas da interação genótipo x ambiente (GA) em progênies de sorgo forrageiro desenvolvidas pelo IPA - Instituto Agronômico de Pernambuco.

Resumo: Em face da instabilidade climática do semiárido brasileiro, notadamente quanto à escassez e a distribuição errática das chuvas, o sorgo forrageiro que apresenta características de xerofilia detém a vantagem sobre a maioria das plantas forrageiras recomendadas para a região como suporte a pecuária, no atendimento de volumosos no período estival do ano. Foi utilizada a metodologia AMMI - ?Additive Main Effects and Multiplicative Interaction? (Análise de Efeitos Aditivos Principais e Interação Multiplicativa) que investiga a interação genótipo ambiente, e tem sido usada por vários autores e em diversas culturas. O objetivo deste trabalho foi avaliar o comportamento de 30 genótipos de sorgo forrageiro testados em seis ambientes do semi-árido de Pernambuco e de Alagoas usando a técnica AMMI visando recomendação. Os genótipos foram desenvolvidos pelo IPA a partir do método sequencial de pedigree, oriundos de cruzamentos e de autofecundações sucessivas até gerações avançadas. Através da análise infere-se que os genótipos G14, G22, G27 e G5 podem ser considerados estáveis e os genótipos G16 e G7 foram os que mais contribuíram com a interação GA

Desempenho de cultivares de milho em diferentes ambientes do Nordeste brasileiro, na safra 2008/2009.

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Desempenho de híbridos simples de milho no nordeste brasileiro: safra 2008/2009.

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Avaliação de híbridos de milho no Nordeste brasileiro: ensaios realizados no ano agrícola 2008.

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Desempenho de cultivares de milho no nordeste brasileiro: safra 2010/2011.

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Albumin and mammalian cell culture: implications for biotechnology applications

Albumin has a long historical involvement in design of media for the successful culture of mammalian cells, in both the research and commercial fields. The potential application of albumins, bovine or human serum albumin, for cell culture is a by-product of the physico-chemical, biochemical and cell-specific properties of the molecule. In this review an analysis of these features of albumin leads to a consideration of the extracellular and intracellular actions of the molecule, and importantly the role of its interactions with numerous ligands or bioactive factors that influence the growth of cells in culture: these include hormones, growth factors, lipids, amino acids, metal ions, reactive oxygen and nitrogen species to name a few. The interaction of albumin with the cell in relation to these co-factors has a potential impact on metabolic and biosynthetic activity, cell proliferation and survival. Application of this knowledge to improve the performance in manufacturing biotechnology and in the emerging uses of cell culture for tissue engineering and stem cell derived therapies is an important prospect

Oxidative protein labeling in mass-spectrometry-based proteomics

Oxidation of proteins and peptides is a common phenomenon, and can be employed as a labeling technique for mass-spectrometry-based proteomics. Nonspecific oxidative labeling methods can modify almost any amino acid residue in a protein or only surface-exposed regions. Specific agents may label reactive functional groups in amino acids, primarily cysteine, methionine, tyrosine, and tryptophan. Nonspecific radical intermediates (reactive oxygen, nitrogen, or halogen species) can be produced by chemical, photochemical, electrochemical, or enzymatic methods. More targeted oxidation can be achieved by chemical reagents but also by direct electrochemical oxidation, which opens the way to instrumental labeling methods. Oxidative labeling of amino acids in the context of liquid chromatography(LC)–mass spectrometry (MS) based proteomics allows for differential LC separation, improved MS ionization, and label-specific fragmentation and detection. Oxidation of proteins can create new reactive groups which are useful for secondary, more conventional derivatization reactions with, e.g., fluorescent labels. This review summarizes reactions of oxidizing agents with peptides and proteins, the corresponding methodologies and instrumentation, and the major, innovative applications of oxidative protein labeling described in selected literature from the last decade