5 research outputs found
Estrazione di licopene con CO2 supercritica da matrici biologiche preparate da bacche mature di pomodoro
Una storia etiopica, l'impresa Varnero
L'intervista ad Alberto Varnero, titolare dell'omonima impresa di costruzioni edili è uno spaccato di storia dell'urbanistica di Addis Abeba dal dopoguerra ad oggi
Isoprenoid, Lipid, and Protein Contents in Intact Plastids Isolated from Mesocarp Cells of Traditional and High-Pigment Tomato Cultivars at Different Ripening Stages
Isoprenoid, Lipid, and Protein Contents in Intact Plastids Isolated from Mesocarp Cells of Traditional and High-Pigment Tomato Cultivars at Different Ripening Stages
This study reports quali-quantitative analyses on isoprenoids, phospholipids, neutral lipids, phytosterols, and proteins in purified plastids isolated from fresh fruits of traditional (Donald and Incas) and high-pigment (Kalvert and HLY-18) tomato cultivars at four ripening stages. In all of the investigated cultivars, lycopene, beta-catotene, lutein, and total carotenoids varied significantly during ripening. Chromoplasts of red-ripe tomato fruits of high-pigment cultivars accumulated twice as much as lycopene (307.6 and 319.2 mu g/mg of plastid proteins in Kalvert and HLY-18, respectively) than ordinary cultivars (178.6 and 151.7 mu g/mg of plastid proteins in Donald and Incas, respectively); differences in chlorophyll and a-tocopherol contents were also evidenced. Phospholipids and phytosterols increased during ripening, whereas triglycerides showed a general decrease. Regardless of the stage of ripening, palmitic acid was the major fatty acid in all cultivars (ranging from 35 to 52% of the total fatty acids), followed by stearic, oleic, linoleic, linolenic, and myristic acids, but their relative percentage was affected by ripening. Most of the bands detected on the SDS-PAGEs of plastid proteins were constantly present during chloroplast-to-chromoplast conversion, some others disappeared, and only one, with a molecular weight of similar to 41.6 kDa, was found to increase in intensity