Biologia de Rhopalosiphum Padi (Hemiptera:Aphididae) em diferentes temperaturas e cultivares de trigo.

Editores técnicos: Joseani Mesquita Antunes, Ana Lídia Variani Bonato, Márcia Barrocas Moreira Pimentel

Uso da técnica EPG para monitoramento do comportamento alimentar de afídeos nas cultivares de trigo Embrapa 16 e BRS Timbaúva.

Editores técnicos: Joseani Mesquita Antunes, Ana Lídia Variani Bonato, Márcia Barrocas Moreira Pimentel

Comparison of three different immunoassays in the diagnosis of heparin-induced thrombocytopenia.

Background: Heparin-induced thrombocytopenia (HIT) is caused by platelet activating antibodies that recognize platelet factor 4/heparin (PF4/H) complexes. Laboratory testing plays a key role in the diagnosis of HIT. As functional assays are unfeasible for most clinical laboratories, antigen binding assays are commonly used in routine testing. However, their low specificity leads to overdiagnosis of HIT. Therefore, it is advisable to improve screening tests in this setting. Methods: Blood samples from 114 patients in whom HIT was suspected were investigated using a chemiluminescence test (HemosIL (R) AcuStar HIT-IgG), a PF4/H IgG enzyme immunoassay (Lifecodes PF4 IgG), an IgG-specific lateral flow immunoassay heparin-induced thrombocytopenia (LFI-HIT, STic Expert (R) HIT) and the heparin-induced platelet aggregation (HIPA) test. Results: Twenty-nine (25.4%) out of 114 subjects with suspected HIT had a positive HIPA test. None of patients with a 4Ts score <4 were positive at HIPA. HemosIL (R) AcuStar HIT-IgG showed the best performance in term of sensitivity and specificity when used as single test. Receiver operating characteristic (ROC) analysis showed optimization of sensitivity and specificity using a cut-off of 1.13 U/mL (0.95 and 0.98, respectively). As an alternative approach, a strategy based on screening samples by STic Expert (R) HIT and then retesting positive results by Lifecodes PF4 IgG (cut-off 1 OD) or HemosIL (R) AcuStar HIT-IgG (cut-off 1.3 U/mL) showed a performance compared to a single test approach by HemosIL (R) AcuStar HIT-IgG. Conclusions: The HemosIL (R) AcuStar HIT or a combinatorial approach with the STic Expert (R) HIT and the PF4/H IgG enzyme immunoassay provide an accurate diagnosis of immune HIT

New Technique of Stereolithography to Local Curing in Thermosensitive Resins Using CO(2) Laser

A theoretical and experimental study of thermosensitive resins used in thermal stereolithography is presented. The process of local curing through the application of infrared radiation, which has proved to be useful in a new technique for the making of prototypes by means of selective heating with C02 laser (10.6um), is studied. The ideal composition of the thermosensitive resins has proved to be 10 parts epoxy, 1.4 part diethylene triamine (the curing agent) and 0.7 part silica powder. A physical theoretical model is applied for control of the parameters which influence the confinement of the curing in the irradiated bulk. A mathematical model is applied too; it was developed through the resolution of the heat conduction equation dependent on time in cylindrical co-ordinates, which enables to determine the behaviour of curing in terms of irradiation conditions

Flutuação populacional de Plutella xylostella (L.) (Lepidoptera: Plutellidae) na cultura da canola, em Passo Fundo - RS.

Resumo: Dentre as pragas que infestam a cultura da canola destaca-se a traça-das-crucíferas, Plutella xylostella (L.). Suas larvas consomem principalmente folhas, mas, em altas populações, também podem consumir hastes e epiderme das síliquas, podendo comprometer a produção de grãos. Estudos que abordem a dinâmica dessa praga na canola são escassos no Brasil. Portanto, este trabalho teve por objetivo realizar a flutuação populacional de P. xylostella nessa cultura no estado do Rio Grande do Sul. O estudo foi conduzido numa área experimental da Embrapa Trigo, Passo Fundo/RS, onde foi semeada uma área de 800 m2 com canola, híbrido Hyola 433, em maio de 2016. Nessa área, foi definida uma área de 125 m2, da qual, semanalmente, de junho a outubro de 2016, 25 plantas de canola foram coletadas e transportadas para o laboratório. As plantas foram vistoriadas com o auxílio de microscópio estereoscópico e as larvas quantificadas. Variáveis climáticas foram obtidas a partir de Estação Meteorológica localizada na Embrapa Trigo. No total, foram coletadas 106 larvas de P. xylostella nas plantas de canola, com o pico populacional observado no mês de outubro (17 larvas). A população de larvas coletadas e a sua densidade por planta foram baixas durante o período avaliado devido em parte, às baixas médias de temperatura. A flutuação populacional de P. xylostella não sofreu influência das precipitações pluviométricas. Abstract: Among the pests that infest the canola crop, the diamondback moth, Plutella xylostella (L.) stands out. The larvae consume mainly leaves, and in high populations they can also consume stems and siliqua, which can compromise grain production. Studies addressing the dynamics of this canola pest are scarce in Brazil. Therefore, this work aimed to carry out the population fluctuation of P. xylostella in this crop in the state of Rio Grande do Sul. The study was conducted in an experimental area of Embrapa Trigo, in Passo Fundo/RS, where an area of the 800 m2 with canola, Hyola 433 hybrid, was planted in May 2016. In this area, 125 m2 were defined and weekly, from June to October 2016, 25 canola plants were collected and examined in the laboratory. Plants were inspected using a stereoscopic microscope and the larvae quantified. Climatic variables were obtained from the Meteorological Station located at Embrapa Trigo. A total of 106 larvae of were collected from canola plants, with the peak population observed in October (17 larvae). The total population of larvae and larvae/plant, were low throughout the period evaluated, due in part to the low average temperatures. The population fluctuation was not influenced by rainfall that occurred during the study

Flutuação populacional de afídeos na cultura da canola, em Passo Fundo - RS.

Dentre as pragas que infestam a cultura da canola destacam-se os afídeos, Brevicoryne brassicae, Lipaphis pseudobrassicae, Macrosiphum euphorbiae e Myzus persicae, que ao se alimentarem da seiva das plantas provocam deformações e enrolamento das folhas, e em infestações severas podem ocasionar a morte das plantas. Estudos que abordem a dinâmica de afídeos na canola são escassos no Brasil. Portanto, este trabalho teve por objetivo realizar a flutuação populacional de afídeos nessa cultura no estado do Rio Grande do Sul, o maior produtor de canola do país. O estudo foi conduzido numa área experimental da Embrapa Trigo, Passo Fundo/RS, onde foi semeada uma área de 800 m2 com canola, híbrido Hyola 433, em maio de 2015. Nessa área, foi definida uma área de 125 m2 , da qual, semanalmente, de maio a setembro de 2015, 25 plantas de canola eram coletadas e transportadas em sacos plásticos para o laboratório de Entomologia. Nesse laboratório, os afídeos foram coletados das plantas, quantificados e identificados em nível de espécie. Variáveis climatológicas foram obtidas a partir de Estação Meteorológica localizada na Embrapa Trigo. No total, foram coletados 2024 espécimes, M. persicae (58%), L. pseudobrassicae (41%) e M. euphorbiae (1%). Os picos populacionais foram: M. persicae em junho (296 espécimes), L. pseudobrassicae em setembro (238) e M. euphorbiae em maio (26). As populações de afídeos foram baixas durante todo o período avaliado. A ocorrência de temperaturas amenas e de precipitações bem distribuídas durante o estudo contribuíram para que as populações das espécies de afídeos não fossem elevadas. ABSTRACT- Among the pests that infest the culture of canola, aphid species Brevicoryne brassicae, Lipaphis pseudobrassicae, Macrosiphum euphorbiae and Myzus persicae stand out, which when feeding on the sap cause deformation and curling of the leaves, and in severe infestations they can cause death of the plants. Studies that address the dynamics of aphids in canola are scarce in Brazil. Therefore, this work aimed to carry out the population fluctuation of aphids in this crop in the state of Rio Grande do Sul, the largest producer of canola in the country. The study was conducted in an experimental area of Embrapa Trigo, in Passo Fundo/RS, where an area of the 800 m2 with canola, Hyola 433 hybrid, was planted in May 2015. In this area, an area of the 125 m2 was defined, of which weekly, from May to September 2015, 25 canola plants were collected and transported in plastic bags to the Entomology laboratory. In this laboratory, aphids were collected from plants, quantified and identified at the species level. Climatological variables were obtained from the Meteorological Station located at Embrapa Trigo. In total, 2024 specimens were collected, M. persicae (58%), L. pseudobrassicae (41%) and M. euphorbiae (1%). The population peaks were: M. persicae in June (296 specimens), L. pseudobrassicae in September (238) and M. euphorbiae in May (26). Aphid populations were low throughout the evaluated period. The occurrence of mild temperatures and well-distributed rainfall during the study contributed so that the populations of aphid species were not high