2,384 research outputs found
Dihedral-angle Gaussian distribution driving protein folding
The proposal of this paper is to provide a simple angular random walk model
to build up polypeptide structures, which encompass properties of dihedral
angles of folded proteins. From this model, structures will be built with
lengths ranging from 125 up to 400 amino acids for the different fractions of
secondary structure motifs, which dihedral angles were randomly chosen
according to narrow Gaussian probability distributions. In order to measure the
fractal dimension of proteins three different cases were analyzed. The first
contained alpha-helix structures only, the second beta-strands structures and
the third a mix of alpha-helices and beta-sheets. The behavior of proteins with
alpha-helix motifs are more compacted than in other situations. The findings
herein indicate that this model describes some structural properties of a
protein and suggest that randomness is an essential ingredient but proteins are
driven by narrow angular Gaussian probability distributions and not by
random-walk processes.Comment: 9 pages, 4 figures, to be published in Physica
Improved method for determination of waxes in olive oils: Reduction of silica and use of a less hazardous solvent
The evaluation of the content of waxes is request both by IOC Trade Standard and by Regulation (EEC) 2568/91 and its further amendments. The official method uses 15 g of silicic acid and elutes several fractions by using huge volumes of dangerous solvent (n-hexane). The developed method uses 1 g of silicic acid with a different particle size and less than 20 mL of solvent mixture, substituting n-hexane with less toxic isooctane. Briefly, after spiking with a suitable internal standard, oil sample is fractionated by SPE (Solid Phase Extraction) cartridge with 1 g of silica, waxes are eluted with 14 mL of isooctane/ethyl ether 99/1 (6 mL discarded and 8 mL collected), then, after elution sample is reconstitute in 200 \u3bcL of n-heptane and analysed by capillary GC. Data of "In home" validation, (repeatability, accuracy and recovery) and relative chromatograms are reported in this paper
Nanoparticles for fingermark detection: an insight into the reaction mechanism
This publication presents one of the first uses of silicon oxide nanoparticles to detect fingermarks. The study is not confined to showing successful detection of fingermarks, but is focused on understanding the mechanisms involved in the fingermark detection process. To gain such an understanding, various chemical groups are grafted onto the nanoparticle surface, and parameters such as the pH of the solutions or zeta potential are varied to study their influence on the detection. An electrostatic interaction has been the generally accepted hypothesis of interaction between nanoparticles and fingermarks, but the results of this research challenge that hypothesis, showing that the interaction is chemically driven. Carboxyl groups grafted onto the nanoparticle surfaces react with amine groups of the fingermark secretion. This formation of amide linkage between carboxyl and amine groups has further been favoured by catalyzing the reaction with a compound of diimide type. The research strategy adopted here ought to be applicable to all detection techniques using nanoparticles. For most of them the nature of the interaction remains poorly understood
Monitoring and Occurrence of Heavy PAHs in Pomace Oil Supply Chain Using a Double-Step Solid-Phase Purification and HPLC-FLD Determination
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental and processing contaminants generated by both spontaneous and anthropogenic incomplete combustion processes of organic matter. Contamination of PAHs in vegetable oils can result from several factors and processes, including environmental contamination, oil processing, and migration from food contact materials. The determination of PAHs in edible oil presents a challenge because of the complexity of the matrix. Since PAHs are present at lower levels than triglycerides, it is necessary to isolate the compounds of interest from the rest of the matrix. To this purpose, a new purification approach based on a double solid-phase extraction (SPE) step followed by high performance liquid chromatography–fluorometric detector (HPLC-FLD) analysis was developed. The method involves a first purification step by using a 5 g silica SPE cartridge, previously washed with dichloromethane (20 mL), dried completely, and then conditioned with n-hexane (20 mL). The triglycerides are retained by the silica, while the PAH-containing fraction is eluted with a mixture of n-hexane/dichloromethane (70/30, v/v). After evaporation, the residue is loaded on a 5 g amino SPE cartridge and eluted with n-hexane/toluene (70/30, v/v) before HPLC-FLD analysis. The focus was the evaluation of the contribution of the various phases of the pomace oil supply chain in terms of the heavy PAHs (PAH8) concentration. Data collected showed that pomace contamination increased (by 15 times) as storage time increased. In addition, the process of pomace drying, which is necessary to reduce its moisture content before solvent extraction of the residual oil, appeared to significantly contribute to the total heavy PAHs content, with increases in value by up to 75 times
Migration of polypropylene oligomers into ready-to-eat vegetable soups
Polyolefin oligomeric hydrocarbons (POH) are non-intentionally added substances (NIAS) which mainly reside in the polymer (PE, PP) as a consequence of the polymerization process, and that under favorable conditions (high fat content, high temperature, and long contact time) may migrate at high amount from the packaging into the food. The food industry offers a wide range of ready-to-eat products, among these, vegetable soups designed to be stored at refrigeration temperature (for times around 6 weeks), and in most cases to be heated for a few minutes in a microwave oven (into the original container, mostly of PP) before consumption. The present work aimed to study for the first-time migration of POH during the shelf life of these products, including storage at refrigeration temperature and after microwave heating. On-line high-performance liquid chromatography (HPLC)-gas chromatography (GC), followed by flame ionization detection (FID), was applied for POH analysis in a number of ready-to-eat products purchased from the Italian market. Microwave heating determined a variable POH increase ranging from 0.1 to 6.2 mg/kg. Parameters possibly affecting migration such as fat content and heating time were also studied
Fingermark Detection on Thermal Papers: Proposition of an Updated Processing Sequence
The detection of latent fingermarks on thermal papers proves to be particularly challenging because the application of conventional detection techniques may turn the sample dark grey or black, thus preventing the observation of fingermarks. Various approaches aiming at avoiding or solving this problem have been suggested. However, in view of the many propositions available in the literature, it gets difficult to choose the most advantageous method and to decide which processing sequence should be followed when dealing with a thermal paper. In this study, 19 detection techniques adapted to the processing of thermal papers were assessed individually and then were compared to each other. An updated processing sequence, assessed through a pseudo-operational test, is suggested
Potential application of liquid dye penetrants for serial number restoration on firearms
© 2018, © 2018 Australian Academy of Forensic Sciences. Chemical reagents for serial number restoration are designed to exploit the plastic deformation zone below the obliterated serial number. Most techniques in operational use are variants of Fry’s Reagent. This technique uses toxic, corrosive chemicals and requires a skilful operator in the laboratory. Fry’s Reagent can also be destructive of the firearm due to rusting. In an attempt to find a non-toxic, non-destructive alternative to Fry’s Reagent, this project tested the use of liquid dye penetrants (LDPs), a non-destructive examination technique to detect cracks and imperfections used in the welding industry, for the restoration of obliterated serial numbers. Steel plates bearing an obliterated serial number with a range of obliteration depths were subjected to LDP treatment under various treatment conditions. The results using LDPs were negative for all obliteration depths and all test conditions attempted. Some of the serial numbers were subsequently restored successfully with Fry’s reagent, proving that the plastic deformation zone was present on the steel plates below the obliteration. Further work to develop a non-toxic, non-destructive serial number restoration process will be undertaken
Microwave-based technique for fast and reliable extraction of organic contaminants from food, with a special focus on hydrocarbon contaminants
Due to food complexity and the low amount at which contaminants are usually present in food, their analytical determination can be particularly challenging. Conventional sample preparation methods making use of large solvent volumes and involving intensive sample manipulation can lead to sample contamination or losses of analytes. To overcome the disadvantages of conventional sample preparation, many researchers put their efforts toward the development of rapid and environmental-friendly methods, minimizing solvent consumption. In this context, microwave-assisted-extraction (MAE) has obtained, over the last years, increasing attention from analytical chemists and it has been successfully utilized for the extraction of various contaminants from different foods. In the first part of this review, an updated overview of the microwave-based extraction technique used for rapid and efficient extraction of organic contaminants from food is given. The principle of the technique, a description of available instrumentation, optimization of parameters affecting the extraction yield, as well as integrated techniques for further purification/enrichment prior to the analytical determination, are illustrated. In the second part of the review, the latest applications concerning the use of microwave energy for the determination of hydrocarbon contaminants-namely polycyclic aromatic hydrocarbons (PAHs) and mineral oil hydrocarbons (MOH)-are reported and critically overviewed and future trends are delineated
Conversion of Fe−NH_2 to Fe−N_2 with release of NH_3
Tris(phosphine)borane ligated Fe(I) centers featuring N_2H_4, NH_3, NH_2, and OH ligands are described. Conversion of Fe–NH_2 to Fe–NH_3^+ by the addition of acid, and subsequent reductive release of NH_3 to generate Fe–N_2, is demonstrated. This sequence models the final steps of proposed Fe–mediated nitrogen fixation pathways. The five-coordinate trigonal bipyramidal complexes described are unusual in that they adopt S = 3/2 ground states and are prepared from a four-coordinate, S = 3/2 trigonal pyramidal precursor
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