573 research outputs found
Chromosomal integration of blaCTX-M genes in diverse Escherichia coli isolates recovered from river water in Japan
Occurrence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBLEC) in environmental waters is of great concern. However, unlike clinical ESBLEC, their genetic characteristics, in particular the genetic contexts of ESBL genes, are not well understood. In this study, we sequenced and analyzed the genomes of CTX-M-producing E. coli isolates recovered from river water to fully characterize the genetic contexts of blaCTX-M genes. Among the 14 isolates with completed genomes, blaCTX-M genes were detected on the chromosome in nine isolates. All but one chromosomal blaCTX-M genes were associated with ISEcp1 and were carried on different transposition units ranging in size from 2, 855 bp to 11, 093 bp; the exception, blaCTX-M-2, was associated with ISCR1. The remaining five isolates carried blaCTX-M genes on epidemic IncI1 plasmids of different sequence types (STs) (ST3, ST16, ST113, and ST167) (n = 4) or on an IncB/O/K/Z plasmid (n = 1). This study revealed that environmental E. coli carry blaCTX-M genes in diverse genetic contexts. Apparent high prevalence of chromosomal blaCTX-M potentially indicates that some E. coli can stably maintain blaCTX-M genes in environmental waters, though further studies are needed to confirm this
Web-based environment for user generation of spoken dialog for virtual assistants
In this paper, a web-based spoken dialog generation environment which enables users to edit dialogs with a video virtual assistant is developed and to also select the 3D motions and tone of voice for the assistant. In our proposed system, “anyone” can “easily” post/edit contents of the dialog for the dialog system. The dialog type corresponding to the system is limited to the question-and-answer type dialog, in order to avoid editing conflicts caused by editing by multiple users. The spoken dialog sharing service and FST generator generates spoken dialog content for the MMDAgent spoken dialog system toolkit, which includes a speech recognizer, a dialog control unit, a speech synthesizer, and a virtual agent. For dialog content creation, question-and-answer dialogs posted by users and FST templates are used. The proposed system was operated for more than a year in a student lounge at the Nagoya Institute of Technology, where users added more than 500 dialogs during the experiment. Images were also registered to 65% of the postings. The most posted category is related to “animation, video games, manga.”
The system was subjected to open examination by tourist information staff who had no prior experience with spoken dialog systems. Based on their impressions of tourist use of the dialog system, they shortened the length of some of the system’s responses and added pauses to the longer responses to make them easier to understand
MMDAE : Dialog scenario editor for MMDAgent on the web browser
We have developed MMDAgent (a fully open-source toolkit for voice interaction systems), which runs on a variety of platforms such as personal computers and smartphones. From this, the editing environment of the dialog scenario also needs to be operated on various platforms. So, we develop a scenario editor that is implemented on a Web browser. The purpose of this paper also includes making it easy to edit the scenario. Experiments were conducted for subjects using the proposed scenario editor. It was found that our proposed system provides better readability of a scenario and allows easier editing
Particle Propagator of Spin Calogero-Sutherland Model
Explicit-exact expressions for the particle propagator of the spin 1/2
Calogero-Sutherland model are derived for the system of a finite number of
particles and for that in the thermodynamic limit. Derivation of the expression
in the thermodynamic limit is also presented in detail. Combining this result
with the hole propagator obtained in earlier studies, we calculate the spectral
function of the single particle Green's function in the full range of the
energy and momentum space. The resultant spectral function exhibits power-law
singularity characteristic to correlated particle systems in one dimension.Comment: 43 pages, 6 figure
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