50 research outputs found
Electromagnetic characterization of soil-litter media – Application to the simulation of the microwave emissivity of the ground surface in forests
In order to improve our knowledge of the emitted signal of forests at L-band (1.4 GHz) we focused this study on permittivity measurements of heterogenic natural media such as soil or litter consisting of plant debris and organic matter. This study was done in the context of the upcoming SMOS (Soil Moisture and Ocean Salinity) satellite mission that will attempt to map surface soil moisture from L-band (1.4 GHz) passive microwave measurements. In the field of passive microwaves, very little information exists about the behaviour of the L-band signal of forests especially when litter is included in the soil-vegetation system. To date very few analyses have investigated the dielectric behaviour of the litter layer and its influence on the microwave emission of forests is generally neglected. © 2008 EDP Sciences
A New Bifidobacteria Expression SysTem (BEST) to Produce and Deliver Interleukin-10 in Bifidobacterium bifidum
In the last years there has been a growing interest in the use of genetically modified bacteria to deliver molecules of therapeutic interest at mucosal surfaces. Due to the well-recognized probiotic properties of some strains, bifidobacteria represent excellent candidates for the development of live vehicles to produce and deliver heterologous proteins at mucosal surfaces. However, very few studies have considered this genus because of its complexity to be genetically manipulated. In this work, we report the development of a new Bifidobacteria Expression SysTem (BEST) allowing the production of heterologous proteins in Bifidobacterium bifidum. This system is based on: i) the broad host range plasmid pWV01, ii) a stress-inducible promoter, and iii) two different signal peptides (SPs) one issued from Lactococcus lactis (SPExp4) and issued from Bifidobacterium longum (SPBL1181). The functionality of BEST system was validated by cloning murine interleukin-10 (IL-10) and establishing the resulting plasmids (i.e., pBESTExp4:IL-10 and pBESTBL1181:IL-10) in the strain of B. bifidum BS42. We then demonstrated in vitro that recombinant B. bifidum BS42 harboring pBESTBL1181:IL-10 plasmid efficiently secreted IL-10 and that this secretion was significantly higher (sevenfold) than its counterpart B. bifidum BS42 harboring pBESTExp4:IL-10 plasmid. Finally, we validated in vivo that recombinant B. bifidum strains producing IL-10 using BEST system efficiently delivered this cytokine at mucosal surfaces and exhibit beneficial effects in a murine model of low-grade intestinal inflammation
Effect of multiple scattering on the phase signature of wet subsurface structures: applications to polarimetric L- and C-band SAR
We propose a two-layer integral equation model (IEM) model including multiple-scattering terms to reproduce the phase signature of buried wet structures that we observed on L-band synthetic aperture radar (SAR) images. We have good agreement between the extended (single+multiple scattering) IEM model and previous results obtained using a single-scattering IEM model combined with finite-difference time-domain simulations. We show that the multiple scattering not only significantly influences the copolarized phase difference but can also be related to the soil moisture content. In order to assess the validity of our extended model, we performed radar measurements on a natural outdoor site and showed that they could be fairly well fitted to the extended model. A parametric analysis presents the dependence of the copolarized phase difference on roughness parameters (rms height and correlation length) and radar parameters (frequency and incidence angle). Our study also shows that the phase signature should allow detection of buried wet structures down to a larger depth for C-band (3.8 m) than for L-band (2.6 m). This signature could then be used to map subsurface moisture in arid regions using polarimetric SAR systems
Radiomètre en ondes millimétriques pour l'étude de l'atmosphère. Utilisation d'éléments quasi-optiques
We present a millimeter-wave radiometric system to study oxygen and water vapor atmospheric lines between 100 and 200 GHz. After a description of the remote sensing principle, we describe some elements of the receiver. The quasi-optical part of the receiver is emphasized : filter, interferometer, polarization rotator.Nous présentons un système radiométrique en ondes millimétriques pour l'étude des raies atmosphériques de l'oxygène et de la vapeur d'eau situées entre 100 et 200 GHz. Après une description du principe du radiosondage, les divers éléments du récepteur sont exposés. L'accent est porté sur les tehniques quasi-optiques utilisées : filtre, interféromètre, rotateur de polarisation
Radiomètre en ondes millimétriques pour l'étude de l'atmosphère. Utilisation d'éléments quasi-optiques
Nous présentons un système radiométrique en ondes millimétriques pour l'étude des raies atmosphériques de l'oxygène et de la vapeur d'eau situées entre 100 et 200 GHz. Après une description du principe du radiosondage, les divers éléments du récepteur sont exposés. L'accent est porté sur les tehniques quasi-optiques utilisées : filtre, interféromètre, rotateur de polarisation