Open Science: a revolution in sight?

• Purpose This article aims at describing the evolution of scientific communication, largely represented by the publication process. It notes the disappearance of the traditional publication on paper and its progressive replacement by electronic publishing, a new paradigm implying radical changes in the whole mechanism. It aims also at warning the scientific community about the dangers of some new avenues and why, rather than subcontracting an essential part of its work, it must take back a full control of its production. • Design/methodology/approach The article reviews the emerging concepts in scholarly publication and aims to answer frequently asked questions concerning free access to scientific literature as well as to data, science and knowledge in general. • Findings The article provides new observations concerning the level of compliance to institutional open access mandates and the poor relevance of journal prestige for quality evaluation of research and researchers. The results of introducing an open access policy at the University of Liège are noted. • Social implications Open access is, for the first time in human history, an opportunity to provide free access to knowledge universally, regardless of either the wealth or the social status of the potentially interested readers. It is an essential breakthrough for developing countries. • Value Open access and Open Science in general must be considered as common values that should be shared freely. Free access to publicly generated knowledge should be explicitly included in universal human rights. There are still a number of obstacles hampering this goal, mostly the greed of intermediaries who persuade researchers to give their work for free, in exchange for prestige. The worldwide cause of Open Knowledge is thus a major universal issue for the 21st Century

Open Science and Covid-19: An opportunity to democratise knowledge?

Researchers face the following dilemma on a daily basis, which the pandemic has brought to the attention of the general public: How can the results of publicly funded research on issues of major importance to humanity be made available to society as a whole, when the major publishers are sequestering this work? Pre-publication platforms have seen a spectacular increase in the number of articles submitted during the Covid-19 pandemic. This success confirms that, when urgency prevails, it is these rapid tools that researchers turn to first, both to inform themselves and to inform. Pre-publications also accelerate the establishment of international collaborations that allow for the rapid compilation of a large volume of epidemiological information and the manipulation of a gigantic amount of data accumulated by many scientists.Les chercheurs sont quotidiennement confrontés au dilemme suivant, que la pandémie a porté à l'attention du grand public : Comment mettre à la disposition de la société dans son ensemble les résultats de recherches financées par des fonds publics sur des questions d'importance majeure pour l'humanité, alors que les grands éditeurs séquestrent ces travaux ? Les plateformes de prépublication ont connu une augmentation spectaculaire du nombre d'articles soumis pendant la pandémie de Covid-19. Ce succès confirme que, lorsque l'urgence prévaut, c'est vers ces outils rapides que les chercheurs se tournent en priorité, tant pour s'informer que pour informer. Les prépublications accélèrent également la mise en place de collaborations internationales qui permettent de compiler rapidement un grand volume d'informations épidémiologiques et de manipuler une quantité gigantesque de données accumulées par de nombreux scientifiques

Preparation of reproducible alkaline phosphatase-antibody conjugates for enzyme immunoassay using a heterobifunctional linking agent

peer reviewedConjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency of such conjugates can be improved by optimizing the degree of substitution of IgG and AP. We have determined conditions yielding better performing conjugates than those synthesized by methods described previously. Moreover, the results obtained with the technique presented here are quite reproducible with all four monoclonal antibodies tested

Recognition of the latency-associated immediate early protein IE63 of varicella-zoster virus by human memory T-lymphocytes

peer reviewedVaricella-zoster virus (VZV) is a human alpha herpesvirus that establishes latency in sensory ganglia. Latency is characterized by the abundant expression of the immediate early protein 63 (IE63), whereas other viral proteins have not yet been detected during the quiescent phase of VZV infection. The IE63 protein is a component of the virion and is expressed very early in the infectious cycle. The IE63 protein is also expressed in skin during episodes of varicella and herpes zoster. We have evaluated the cell-mediated immune response against IE63 in naturally immune adults with a history of chickenpox, by T lymphoproliferation and cytotoxicity assays. Among donors who had T cell proliferation to unfractionated VZV Ags from infected cell extract, 59% had T cell recognition of purified IE63. The CTL response to IE63 was equivalent to CTL recognition of IE62, the major tegument component of VZV whose immunogenicity has been previously described. IgG Abs against IE63 were detected in serum from healthy immune adults. These results indicate that IE63 is an important target of immunity to VZV. T cell recognition of IE63 is likely to be involved in controlling VZV reactivation from latency

Etude des plaques de Peyer de l'intestin du souriceau nouveau-né comme voie possible de pénétration du virus du cancer mammaire de la souris (MMTV)

Peer reviewe

Acute experimental glomerulonephritis induced by the glomerular deposition of circulating polymerid IgA-Concanavalin A complexes

The perfusion of polymeric or secretory IgA-Concanavalin A complexes into the aorta of rats led to a mannose-dependent binding of both IgA and lectin to the glomerular capillary wall, as shown by double immunolocalization experiments, by quantitative analysis of the amount of radiolabeled complexes bound per g of kidney, and by blocking experiments with the corresponding carbohydrate. Rats injected with amounts of those complexes as low as 500 ?g developed, one hour later, a focal and segmental proliferative glomerulonephritis characterized by the deposition of injected complexes and of rat C3 and rat fibrin/ fibrinogen in most glomeruli ; focal thrombosis and small areas of necrosis in 10 to 15% of glomeruli, confined to the periphery of a single lobule of the tuft and segmental infiltration of these glomeruli by polymorphonuclear leucocytes and platelets. At the same time, many mesangial cells exhibited a hyperactive appearance, and red blood cells were noted in tubular lumens. In contrast, rats similarly injected with either monomeric IgA-ConA complexes, multimeric or secretory IgA-peanut agglutinin complexes or polymeric or monomeric IgA aggregates of comparable apparent molecular weight did not develop obvious glomerular lesions within one hour. The data indicate that preformed polymeric IgA-ConA complexes can specifically bind to glomerular structures in vivo and trigger acute glomerular lesions locally, analogous to those observed in some glomerular diseases associated with a cryoglobulinemia

Varicella vaccination in Japan, South Korea, and Europe.

The most extensive use of varicella vaccine has been in the United States and Canada, where it is universally recommended. However, a number of other countries now have recommendations for use of the vaccine, which has been expanding in Europe and Latin America. In this article, we review information concerning varicella vaccination in Japan, where the vaccine was first developed, and in South Korea and parts of Europe. Despite the worldwide availability of an efficient vaccine, varicella vaccination policy is highly variable from country to country. The recent development of a tetravalent vaccine against measles, mumps, rubella, and varicella could modify this variability in the future. It is evident that efforts to control varicella will spread gradually to all continents