Percentages of T helper (CD3+CD8−) producing IFN-γ (Th1 - A/D) and IL-17 (Th17 - B/E), and percentages of Treg (CD3+CD4+CD127-Foxp3+ - C) lymphocytes in healthy volunteers and septic patients with paired samples (D0 and D7). n = number of individuals in each group.

<p><i>P</i> values are shown. The Mann-Whitney test was used to compare healthy volunteer and patient samples (D0 and D7), and the Wilcoxon Signed-Rank Test was used to compare D0- and D7-related samples.</p

Percentages of T helper (CD3+CD8−) lymphocytes producing IFN-γ and IL-17, T helper (CD3+CD4+) expressing CD127-Foxp3+ and monocytes (CD14 positive) expressing CD163 or CD206 in healthy volunteers and septic patients (D0 samples).

<p>Values represent the median and 25–75% quartile. n  =  number of individuals in each group. The Mann-Whitney test was applied.</p

Percentages of T helper lymphocytes producing IFN-γ, IL-17 (CD3+CD8−), T helper (CD3+CD4+) expressing CD127−Foxp3+ and monocytes (CD14 positive) expressing CD163 or CD206 in septic patients at admission (D0), divided into non-survivors (n = 24) and survivors (n = 35). Values represent the median and 25–75% quartile.

<p>The Mann-Whitney test was applied.</p

Strategy for the analysis of alternatively activated monocytes.

<p>Dot plots shown are representative of one healthy volunteer and one septic patient. Twenty thousand events were acquired based on forward scatter versus side scatter parameters and CD14-positive stained cells. The percentages of cells expressing CD163 or CD206 were determined with quadrants based on the isotype controls.</p

Strategy for the analysis of T regulatory lymphocytes.

<p>Dot plots shown are representative of one healthy volunteer and one septic patient. Fifty thousand events were acquired with CD3+ gating and forward scatter versus side scatter parameters. For the analyses, the first gate was determined based on low SSC and high CD45 APC-Cy7, and the second gate was based on CD4+CD3+ cells. The cells with the phenotype CD45+CD3+CD4+CD127-FOXP3+ were considered to be Treg lymphocytes. Quadrants or gates were established based on the isotype controls.</p

Percentages of AAM expressing CD163 (A) and CD206 (B) in healthy volunteers and septic patients with paired samples (D0 and D7).

<p>n =  number of individuals in each group. <i>P</i> values are shown. The Mann-Whitney test was used to compare healthy volunteer and patient samples (D0 and D7), and the Wilcoxon Signed-Rank Test was applied to patients’ related samples.</p

Absolute counts of CD4+ T lymphocytes and subpopulations (CD3+CD8−) producing IFN-γ and IL-17 after PMA/Io stimulation, and T helper (CD3+CD4+) expressing CD127-Foxp3+ in healthy volunteers (n = 30) and septic patients (D0 samples, n = 46).

<p>Values represent the median and 25–75% quartile. The Mann-Whitney test was applied.</p

Percentages of T helper (CD3+CD8−) producing IFN-γ (Th1 - A/D) and IL-17 (Th17 - B/E), and percentages of Treg (CD3+CD4+CD127Foxp3+ - C) lymphocytes in non-survivors (n = 14) and survivors (n = 17) at admission (D0) and after 7 days (D7) in patients with follow-up samples. *<i>P</i><0.05 (Wilcoxon Signed-Rank Test).

<p>Percentages of T helper (CD3+CD8−) producing IFN-γ (Th1 - A/D) and IL-17 (Th17 - B/E), and percentages of Treg (CD3+CD4+CD127Foxp3+ - C) lymphocytes in non-survivors (n = 14) and survivors (n = 17) at admission (D0) and after 7 days (D7) in patients with follow-up samples. *<i>P</i><0.05 (Wilcoxon Signed-Rank Test).</p

Neutrophil reactive oxygen species (ROS) generation in patients across the continuum of sepsis

ROS generation was measured by 2',7'dichlorofluorescein (DCFH) metabolism. Neutrophils were gated based on FSC versus SSC parameters and the expression of CD14 on cell surface. ROS generation was analyzed in histograms and expressed as the geometric mean fluorescence intensity (GMFI). *p < 0.01 compared to healthy volunteers; #p < 0.05 compared to the severe sepsis group; §p < 0.05 compared to the septic shock group.<p><b>Copyright information:</b></p><p>Taken from "Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis"</p><p>http://ccforum.com/content/12/1/R25</p><p>Critical Care 2008;12(1):R25-R25.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2374621.</p><p></p

Correlation between formyl-methionyl-leucyl-phenylalanine (fMLP)-induced reactive oxygen species (ROS) generation and sepsis-associated organ failure assessment (SOFA) score in neutrophils and monocytes in patients with severe sepsis and septic shock

<p><b>Copyright information:</b></p><p>Taken from "Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis"</p><p>http://ccforum.com/content/12/1/R25</p><p>Critical Care 2008;12(1):R25-R25.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2374621.</p><p></p