White spot syndrome virus (WSSV) transmission risk through infected cooked shrimp products assessed by polymerase chain reaction (PCR) and bio-inoculation studies

The aim of the study was to evaluate the resistance of white spot syndrome virus (WSSV) in shrimps (Penaeus monodon) to the process of cooking. The cooking was carried out at 1000C six different durations 5, 10, 15, 20, 25 and 30 min. The presence of WSSV was tested by single step and nested polymerase chain reaction (PCR). In the single step PCR, the primers 1s5 & 1a16 and IK1 & IK2 were used. While in the nested PCR, primers IK1 &IK2 – IK3 & IK4 were used for the detection of WSSV. WSSV was detected in the single step PCR with the primers 1s5 and 1a16 and the nested PCR with the primers IK1 and IK2 – IK3 & IK4 from the cooked shrimp samples. The cooked shrimps, which gave positive results for WSSV by PCR, were further confirmed for the viability of WSSV by conducting the bio-inoculation studies. Mortality (100%) was observed within 123 h of intra-muscular post injection (P.I) into the live healthy WSSV-free shrimps (P. monodon). These results show that the WSSV survive the cooking process and even infected cooked shrimp products may pose a transmission risk for WSSV to the native shrimp farming systems

Numerical Toy-Model Calculation of the Nucleon Spin Autocorrelation Function in a Supernova Core

We develop a simple model for the evolution of a nucleon spin in a hot and dense nuclear medium. A given nucleon is limited to one-dimensional motion in a distribution of external, spin-dependent scattering potentials. We calculate the nucleon spin autocorrelation function numerically for a variety of potential densities and distributions which are meant to bracket realistic conditions in a supernova core. For all plausible configurations the width of the spin-density structure function is found to be less than the temperature. This is in contrast with a naive perturbative calculation based on the one-pion exchange potential which overestimates the width and thus suggests a large suppression of the neutrino opacities by nucleon spin fluctuations. Our results suggest that it may be justified to neglect the collisional broadening of the spin-density structure function for the purpose of estimating the neutrino opacities in the deep inner core of a supernova. On the other hand, we find no indication that processes such as axion or neutrino pair emission, which depend on nucleon spin fluctuations, are substantially suppressed beyond the multiple-scattering effect already discussed in the literature. Aside from these practical conclusions, our model reveals a number of interesting and unexpected insights. For example, the spin-relaxation rate saturates with increasing potential strength only if bound states are not allowed to form by including a repulsive core. There is no saturation with increasing density of scattering potentials until localized eigenstates of energy begin to form.Comment: 14 latex pages in two-column format, 15 postscript figures included, uses revtex.sty and epsf.sty. Submitted to Physical Review

Contact stress analysis of spiral bevel gears using nonlinear finite element static analysis

A procedure is presented for performing three-dimensional stress analysis of spiral bevel gears in mesh using the finite element method. The procedure involves generating a finite element model by solving equations that identify tooth surface coordinates. Coordinate transformations are used to orientate the gear and pinion for gear meshing. Contact boundary conditions are simulated with gap elements. A solution technique for correct orientation of the gap elements is given. Example models and results are presented

Viscous three-dimensional analyses for nozzles for hypersonic propulsion

A Navier-Stokes computer code was validated using a number of two- and three-dimensional configurations for both laminar and turbulent flows. The validation data covers a range of freestream Mach numbers from 3 to 14, includes wall pressures, velocity profiles, and skin friction. Nozzle flow fields computed for a generic scramjet nozzle from Mach 3 to 20, wall pressures, wall skin friction values, heat transfer values, and overall performance are presented. In addition, three-dimensional solutions obtained for two asymmetric, single expansion ramp nozzles at a pressure ratio of 10 consists of the internal expansion region in the converging/diverging sections and the external supersonic exhaust in a quiescent ambient environment. The fundamental characteristics that were captured successfully include expansion fans; Mach wave reflections; mixing layers; and nonsymmetrical, multiple inviscid cell, supersonic exhausts. Comparison with experimental data for wall pressure distributions at the center planes shows good agreement

Kaon Condensation in a Nambu--Jona-Lasinio (NJL) Model at High Density

We demonstrate a fully self-consistent microscopic realization of a kaon-condensed colour-flavour locked state (CFLK0) within the context of a mean-field NJL model at high density. The properties of this state are shown to be consistent with the QCD low-energy effective theory once the proper gauge neutrality conditions are satisfied, and a simple matching procedure is used to compute the pion decay constant, which agrees with the perturbative QCD result. The NJL model is used to compare the energies of the CFLK0 state to the parity even CFL state, and to determine locations of the metal/insulator transition to a phase with gapless fermionic excitations in the presence of a non-zero hypercharge chemical potential and a non-zero strange quark mass. The transition points are compared with results derived previously via effective theories and with partially self-consistent NJL calculations. We find that the qualitative physics does not change, but that the transitions are slightly lower.Comment: 21 pages, ReVTeX4. Clarified discussion and minor change

On some singularities of the correlation functions that determine neutrino opacities

Certain perturbation graphs in the calculation of the effects of the medium on neutrino scattering in supernova matter have a nonintegrable singularity in a physical region. A number of papers have addressed the apparent pathology through an ansatz that invokes higher order (rescattering) effects. Taking the Gamow-Teller terms as an example, we display an expression for the spin-spin correlation function that determines the cross-sections. It is clear from the form that there are no pathologies in the order by order perturbation expansion. Explicit formulae are given for a simple case, leading to an answer that is very different from one given by other authors.Comment: 8 page

On the ground state of gapless two flavor color superconductors

This paper is devoted to the study of some aspects of the instability of two flavor color superconductive quark matter. We find that, beside color condensates, the Goldstone boson related to the breaking of $U(1)_A$ suffers of a velocity instability. We relate this wrong sign problem, which implies the existence of a Goldstone current in the ground state or of gluonic condensation, to the negative squared Meissner mass of the $8^{th}$ gluon in the g2SC phase. Moreover we investigate the Meissner masses of the gluons and the squared velocity of the Goldstone in the multiple plane wave LOFF states, arguing that in such phases both the chromo-magnetic instability and the velocity instability are most probably removed. We also do not expect Higgs instability in such multiple plane wave LOFF. The true vacuum of gapless two flavor superconductors is thus expected to be a multiple plane wave LOFF state.Comment: 16 pages, RevTe3X4 styl

Circular dichroism studies on the signal sequence of E. coli alkaline phosphatase indicate the presence of both α-helix and β-structure in hydrophobic environments

The conformations of a synthetic peptide corresponding to the signal sequence of E. coli alkaline phosphatase, Lys-Gln-Ser-Thr-Ile-Ala-Leu-Ala-Leu-Leu-Pro-Leu-Leu-Phe-Thr-Pro-Val-Thr-Lys-Ala-OCH3, have been examined in different environments by circular dichroism spectroscopy. In trifluoroethanol, methanol and aqueous mixtures of these solvents, the signal peptide has largely random conformation (~80%) with small amounts of a-helix and β-structure. However, in micellar environment, there is a significant increase in ordered conformation with both α-helix and β-structure being present, unlike in other signal sequences reported in the literature, where only the α-helical conformation has been observed. Hence, an α-helical conformation may not be as stringent a requirement as overall hydrophobicity for recognition of signal sequences by the cell's export machinery