Satisfacción en la calidad de la atención prenatal según opinión de las usuarias del centro de salud Primero de Mayo del Municipio de Matagalpa durante el segundo semestre del año 2016

El propósito de la investigación fué analizar la satisfacción en la calidad de atención prenatal según opinión de las usuarias externas e internas del centro de salud Primero de Mayo, durante el ll semestre 2016, analizando el entorno, el aspecto científico-técnico y satisfacción de los usuarios, esté estudio es descriptivo de corte transversal, prospectivo en el tiempo, con un enfoque mixto, el universo lo comprenden 100 embarazadas, con una muestra de 20 gestantes, con edades comprendidas de 16-40 años, el tipo de muestreo utilizado es aleatorio simple, para la valoración de la atención prenatal se realizó entrevistas dirigidas a usuarios externos e internos y una guía de observación para el investigador valorando el entorno de la unidad y realizando revisión del expediente clínico, el centro de salud atiende a 6 barrios de la parte norte de la ciudad de Matagalpa, las debilidades son: no cuentan con equipos suficientes para la realización de procedimientos, las dificultades que enfrenta el puesto son: poco personal, falta de medicamento .En conclusión según observador y usuario interno valoran el entorno como regular, y las usuarias externas lo ven bueno, regular y malo, en cuanto a la atención prenatal, las usuarias externas manifiestan que no se les realiza el examen de mamas lo que hace que la calidad sea deficiente, en cuanto a satisfacción los usuarios internos refieren no estar de acuerdo con la carga laboral, las usuarias externas refieren que el personal de salud no cumple con el horario establecido por el MINS

Prenatal Diagnosis of Cardiac Diverticulum with Pericardial Effusion in the First Trimester of Pregnancy with Resolution after Early Pericardiocentesis

Cardiac diverticulum is a rare anomaly, which may present in association with pericardial effusion. Only few cases diagnosed during fetal life have been published and only in 12 cases pericardiocentesis was made with good postnatal outcomes in 83% of the cases. In the first trimester of pregnancy only 6 cases were reported. We described the largest series of cases published. We describe a case of cardiac diverticulum complicated with pericardial effusion during the first trimester of pregnancy and resolved by intrauterine pericardiocentesis at 17 weeks of pregnancy. We made a systematic review of the literature with the cases reported of cardiac diverticulum, management, and outcomes

Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds

The extracellular matrix (ECM) is a complex network with multiple functions, including specific functions during tissue regeneration. Precisely, the properties of the ECM have been thoroughly used in tissue engineering and regenerative medicine research, aiming to restore the function of damaged or dysfunctional tissues. Tissue decellularization is gaining momentum as a technique to obtain potentially implantable decellularized extracellular matrix (dECM) with well-preserved key components. Interestingly, the tissue-specific dECM is becoming a feasible option to carry out regenerative medicine research, with multiple advantages compared to other approaches. This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.This work was supported by the Spanish “Programa Estatal de I+D+i Orientada a los Retos de la Sociedad”, grant number RTI2018-101708-A-I00. S.R.-C. was supported by Fomento de San Sebastian innovative talent programme, grant number 0508/2019/0009. R.R.-H. was supported by Spanish State Training Subprogramme, grant number PRE2018-084542. A.A. was supported by Spanish State Subprogramme of Incorporation Ramón y Cajal, grant number RYC2018-025502-I, and 2019 Leonardo Grant for Researchers and Cultural Creators, BBVA Foundation, grant number IN[19]_CMA_BIO_0119

Ventilatory Thresholds Estimation Based on ECG-derived Respiratory Rate

The purpose of this work is to study the feasibility of estimating the first and second ventilatory thresholds (VT1 and VT2, respectively) by using electrocardiogram (ECG)-derived respiratory rate during exercise testing. The ECGs of 25 healthy volunteers during cycle ergometer exercise test with increasing workload were analyzed. Time-varying respiratory rate was estimated from an ECG-derived respiration signal obtained from QRS slopes' range method. VT1 and VT2 were estimated as the points of maximum change in respiratory rate slope using polynomial spline smoothing. Reference VT1 and VT2 were determined from the ventilatory equivalents of O2 and CO2. Estimation errors (in watts) of -13.96 (54.84) W for VT1 and -8.06 (39.63) Wfor VT2 (median (interquartile range)) were obtained, suggesting that ventilatory thresholds can be estimated from solely the ECG signal

Estudio de la microestructura y composición química de dos suelos limo-arcillosos, tratados con nano-sílice

En el presente trabajo se lleva a cabo la caracterización de dos suelos limo-arcillosos con contenidos de agua diferentes, recolectados en el Estado de México. Por medio del microscopio electrónico de barrido (MEB) se observaron partículas aglutinantes de nano-sílice adheridas a las partículas de limo y a cenizas volcánicas presentes en los suelos, lo cual modificó y mejoró algunas características mecánicas e hidráulicas del mismo. Por difracción de Rayos X en polvo (DRX) la muestra presenta abundante silicio y aluminio, una gravedad especifica en promedio de 2.4, el tamaño predominante es del orden de 0.01 a 0.001 mm correspondiente al 49% en peso de la muestra analizada.In the present work, the characterization of two silty-clayey soils with different water contents, collected in the State of Mexico, is carried out. Through the scanning electron microscope (SEM) nano-silica binding particles were observed adhered to the silt particles and volcanic ash present in the soils, which modified sorne mechanical and hydraulic characteristics of the same. By X-Ray powder diffraction (XRD) the sample Present abundant silicon and aluminum, an average specific gravity of 2.4, the predominant size is of the arder of 0.01 to 0.001 mm corresponding to 49% by weight of the analyzed sample

Chronic alcohol consumption alters extracellular space geometry and transmitter diffusion in the brain

[EN] Already moderate alcohol consumption has detrimental long-term effects on brain function. However, how alcohol produces its potent addictive effects despite being a weak reinforcer is a poorly understood conundrum that likely hampers the development of successful interventions to limit heavy drinking. In this translational study, we demonstrate widespread increased mean diffusivity in the brain gray matter of chronically drinking humans and rats. These alterations appear soon after drinking initiation in rats, persist into early abstinence in both species, and are associated with a robust decrease in extracellular space tortuosity explained by a microglial reaction. Mathematical modeling of the diffusivity changes unveils an increased spatial reach of extrasynaptically released transmitters like dopamine that may contribute to alcohol's progressively enhanced addictive potencyThis work was supported by the European Union's Horizon 2020 research and innovation program (668863-SyBil-AA) and the ERA-NET NEURON program (FKZ 01EW1112-TRANSALC and PIM2010ERN-00679), as well as the Spanish State Research Agency through the Severo Ochoa Program for Centres of Excellence in R&D (SEV-2017-0723), the Deutsche Forschungsgemeinschaft (center grant TRR265-B08), and the Czech Science Foundation (GACR; grant no. 16-10214S to L.V.). S.C. and D.M. further acknowledge financial support from the Ministerio de Economia y Competitividad (MINECO) and FEDER funds under grant nos. BFU2015-64380-C2-1-R, BFU2015-64380-C2-2-R, and PGC2018-101055-B-I00 and the Generalitat Valenciana through the Prometeo Program (PROMETEO/2019/015). S.C. also acknowledges support of the Ministerio de Sanidad, Servicios Sociales e Igualdad (#2017I065). E.S. acknowledges financial support from the Slovak Research and Development Agency (APVV-17-0642). S.D.S. is supported by a NARSAD Young Investigator Grant (grant no. 25104), by the European Research Council through a Marie Sklodowska-Curie Individual Fellowship (grant no. 749506), and by the Generalitat Valenciana grant SEJI/2019/038. R.C. is supported by the NIAAA grant AA017447. W.H.S acknowledges support from the Bundesministerium fur Bildung und Forschung (BMBF; FKZ: 031L0190A, 01ZX1909CA).De Santis, S.; Cosa-Liñán, A.; Garcia-Hernandez, R.; Dmytrenko, L.; Vargova, L.; Vorisek, I.; Stopponi, S.... (2020). Chronic alcohol consumption alters extracellular space geometry and transmitter diffusion in the brain. Science Advances. 6(26):1-12. https://doi.org/10.1126/sciadv.aba0154S11262

Evaluation of monocytes as carriers for armed oncolytic adenoviruses in murine and Syrian hamster models of cancer

Replication-competent (oncolytic) adenoviruses (OAV) can be adapted as vectors for the delivery of therapeutic genes, with the aim of extending the antitumor effect beyond direct cytolysis. Transgene expression using these vectors is usually intense but short-lived, and repeated administrations are hampered by the rapid appearance of neutralizing antibodies (NAbs). We have studied the performance of monocytes as cell carriers to improve transgene expression in cancer models established in athymic mice and immunocompetent Syrian hamsters. Human and hamster monocytic cell lines (MonoMac6 and HM-1, respectively) were loaded with replication-competent adenovirus-expressing luciferase. Intravenous administration of these cells caused a modest increase in transgene expression in tumor xenografts, but this effect was virtually lost in hamsters. In contrast, intratumoral administration of HM-1 cells allowed repeated cycles of expression and achieved partial protection from NAbs in preimmunized hamsters bearing pancreatic tumors. To explore the therapeutic potential of this approach, HM-1 cells were loaded with a hypoxia-inducible OAV expressing the immunostimulatory cytokine interleukin-12 (IL-12). Three cycles of treatment achieved a significant antitumor effect in the hamster model, and transgene expression was detected following each administration, in contrast with the rapid neutralization of the free virus. We propose monocytes as carriers for multiple intratumoral administrations of armed OAVs

Mapping microglia and astrocyte activation in vivo using diffusion MRI

While glia are increasingly implicated in the pathophysiology of psychiatric and neurodegenerative disorders, available methods for imaging these cells in vivo involve either invasive procedures or positron emission tomography radiotracers, which afford low resolution and specificity. Here, we present a noninvasive diffusion-weighted magnetic resonance imaging (MRI) method to image changes in glia morphology. Using rat models of neuroinflammation, degeneration, and demyelination, we demonstrate that diffusion-weighted MRI carries a fingerprint of microglia and astrocyte activation and that specific signatures from each population can be quantified noninvasively. The method is sensitive to changes in glia morphology and proliferation, providing a quantitative account of neuroinflammation, regardless of the existence of a concomitant neuronal loss or demyelinating injury. We prove the translational value of the approach showing significant associations between MRI and histological microglia markers in humans. This framework holds the potential to transform basic and clinical research by clarifying the role of inflammation in health and disease

A Comprehensive Biomarker Analysis of Microsatellite Unstable/Mismatch Repair Deficient Colorectal Cancer Cohort Treated with Immunotherapy

Biomarkers; Colorectal cancer; ImmunotherapyBiomarcadors; Càncer colorectal; ImmunoteràpiaBiomarcadores; Cáncer colorrectal; InmunoterapiaThe search for immunotherapy biomarkers in Microsatellite Instability High/Deficient Mismatch Repair system (MSI-H/dMMR) metastatic colorectal cancer (mCRC) is an unmet need. Sixteen patients with mCRC and MSI-H/dMMR (determined by either immunohistochemistry or polymerase chain reaction) treated with PD-1/PD-L1 inhibitors at our institution were included. According to whether the progression-free survival with PD-1/PD-L1 inhibitors was longer than 6 months or shorter, patients were clustered into the IT-responder group (n: 9 patients) or IT-resistant group (n: 7 patients), respectively. In order to evaluate determinants of benefit with PD-1/PD-L1 inhibitors, we performed multimodal analysis including genomics (through NGS panel tumour-only with 431 genes) and the immune microenvironment (using CD3, CD8, FOXP3 and PD-L1 antibodies). The following mutations were more frequent in IT-resistant compared with IT-responder groups: B2M (4/7 versus 2/9), CTNNB1 (2/7 versus 0/9), and biallelic PTEN (3/7 versus 1/9). Biallelic ARID1A mutations were found exclusively in the IT-responder group (4/9 patients). Tumour mutational burden did not correlate with immunotherapy benefit, neither the rate of indels in homopolymeric regions. Of note, biallelic ARID1A mutated tumours had the highest immune infiltration and PD-L1 scores, contrary to tumours with CTNNB1 mutation. Immune microenvironment analysis showed higher densities of different T cell subpopulations and PD-L1 expression in IT-responders. Misdiagnosis of MSI-H/dMMR inferred by discordances between immunohistochemistry and polymerase chain reaction was only found in the IT-resistant population (3/7 patients). Biallelic ARID1A mutations and Wnt signalling activation through CTNNB1 mutation were associated with high and low T cell immune infiltrates, respectively, and deserve special attention as determinants of response to PD-1/PD-L1 inhibitors. The non-MSI-H phenotype in dMMR is associated with poor benefit to immunotherapy. Our results suggest that mechanisms of resistance to immunotherapy are multi-factorial.This research was funded by Merck Research Grants (Call 2018) in the Area of Colorectal Cancer Clinical Investigation