Impact of External Factors on Fast Food Business

This paper investigates the impact of external factors on the fast food business. The major objective of this paper is to analyze Political, Economical, Social and Technological factors which affect the performance of fast food business. Qualitative method of research is used. The results show that external factors (political, economical, social and technological) have significant impact on the fast food business. To improve administrative performance and to make business prosper, organizations should focus on the factors which affect their business by taking repeated examination. Given the importance of understanding the PEST analysis with need to fill the niche of research on the topic in our country; this study is of high significance to academia and practitioners in the Hotel Industry. Keywords:  external factors, political, economical, social, technological, administrative performance, fast food

Impact of social interdependence on emotional well-being of medical students

OBJECTIVE:To observe the effect of social interdependence on emotional well-being in medical students.METHODS:This cross-sectional study was conducted in Karachi from June 2013 to January 2014 and comprised first-year medical students of 5 private and 3 public-sector colleges. Students of both genders aged 19-24 years were included. Quantitative aspects of social and emotional wellness were evaluated using a structured questionnaire from the wellness wheel on a four-point Likert\u27s scale (score ranging from 0 to 3). Two focus group discussions were conducted in each medical college from the qualitative aspect.RESULTS:Of the 736 participants, 526(71.47%) were females and 210(28.53%) were males. Males reported significantly less inclination towards exhibiting fairness, solving problems, teaching batch fellows and attending group discussion (p\u3c0.05). They were significantly least anxious with less frequency of helping others during emotional outbursts (p\u3c0.05). Females had higher frequency of close friends and self-improvement (p\u3c0.05). The scores of social wellness were significantly better in females compared to males (p\u3c0.001) while there was no significant difference in emotional wellness on the basis of gender (p\u3e0.05). Social communication and help was highly positively correlated with self-content in students (p\u3c0.05)

Faculty Development Initiatives: A prerequisite for capacity building and enhanced productivity in a medical institution

Objective: To determine the contribution of teaching, learning and assessment forum\u27s initiatives on professional development of faculty and staff.Methods: This retrospective study was conducted at the Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, from July to December 2016, and comprised teaching, learning and assessment activities carried out from 2012 to 2015. The responses acquired from feedback evaluation were recorded at the end of activity on a Likert-type scale ranging from 1-5. Positive responses were presented for each variable with respective activity type across the study years. The association of the effectiveness of teaching, learning and assessment between type of event and yearly outcome was also assessed.Results: A total of 66activities were held during the study period. Of them, 49(74.24%) were workshops/human resource trainings, 5(7.57%) were courses and 12(18.18%) were seminars. Together, they involved over 500 participants. Objectives, disclosure statement, contents, level of interaction, acquired knowledge, time management, queries responded, organisational activity, course material and overall assessment showed consistent positive response across the years, but the acquisition of new knowledge differed significantly (pConclusions: Teaching, learning and assessment initiatives, play a positive role in professional development of faculty and staff

Association between vitamin D, reproductive hormones and sperm parameters in infertile male subjects

Background: The prevalence of infertility and vitamin D deficiency is common in Pakistan. Therefore, our study aims were to assess and compare Vitamin D; 25-hydroxyvitamin (25OHD) and reproductive hormone levels in male fertile and infertile subjects with normal and abnormal sperm parameters. Furthermore, the study is aimed to explore the association of 25OHD levels with these sperm parameters in a selected population of Karachi, Pakistan. Methods: The cross-sectional study was carried out from August 2016 till December 2017, 313 study subjects were recruited from an Infertile Clinic from Islamabad, Pakistan, and the general population. First, we took the couples\u27 history of parenting and then carried out a semen analysis and infertile and fertile male subjects were then subgrouped into normal and altered sperm parameter/s. Forward linear regression was done for selection of 25OHD as a significant predictor of sperm parameters. Results: The median values of the total count, motility, morphology as well as serum 25OHD were significantly higher in the group with normal (186) as compared to subjects (127) in abnormal sperm parameters group. The 25OHD levels were significantly high in males with normal sperm parameters ; 80.90 ± 23.33 nmol/L vs. altered sperm parameter/s, 64.68 ± 24.21 nmol/L (mean ± SD) with p \u3c 0.001. Serum testosterone level had a significant positive correlation with 25OHD while LH had a significant negative correlation with 25OHD (p \u3c 0.001), and FSH level had a non-significant negative correlation with 25OHD. Results of regression model showed one unit increase of motility would give 0.15-unit positive significant impact on 25OHD; 20% variation in 25OHD was explained by the total count, motility, and morphology, while the model was adjusted for BMI. Conclusion: The impact of 25OHD levels on sperm parameters can be emphasized on the basis of detection of its high serum levels in normal subjects in both fertile as well as infertile males in comparison to subjects that had altered sperm parameters; total sperm count, motility, and normal morphology. The considerably positive association between 25OHD, testosterone, total count, motility, and morphology further accentuates its impact on normal spermatogenesis and the male reproductive functions required for acquiring fertility

Plant genome editing using engineered nucleases and success of CRISPR/Cas9 system

Development of new plant breeding techniques have facilitated easy manipulation of plants at genetic level. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein9 (Cas9) system is a valuable addition in programmable nucleases. The CRISPR/Cas9 system uses an RNA component to recognize a target DNA sequences and it has shown promising results with respect to simultaneous editing of multigenic plant traits. In this review, components of CRISPR/Cas9, their construction and its methods of delivery to plant cells are analyzed. Variation in nucleotide sequence of the protospacer adjacent motif, codon optimization and progress in web-based bioinformatic tools, will make CRISPR/Cas9 systems more efficient for plants. Development and optimization of protocols to efficiently target all plant species is still under development. Along with this, methods to inspect induced mutation and efficiency of the system have also been reviewed. Auxiliary improvements and understanding are still required to expand the CRISPR/Cas9 systems to target complex genome architectures and epigenetic elements

Cytostatic Action of Novel Histone Deacetylase Inhibitors in Androgen Receptor-Null Prostate Cancer Cells

Androgen receptor (AR)-null prostate tumors have been observed in 11–24% of patients. Histone deacetylases (HDACs) are overexpressed in prostate tumors. Therefore, HDAC inhibitors (Jazz90 and Jazz167) were examined in AR-null prostate cancer cell lines (PC3 and DU145). Both Jazz90 and Jazz167 inhibited the growth of PC3 and DU145 cells. Jazz90 and Jazz167 were more active in PC3 cells and DU145 cells in comparison to normal prostate cells (PNT1A) and showed a 2.45- and 1.30-fold selectivity and higher cytotoxicity toward DU145 cells, respectively. Jazz90 and Jazz167 reduced HDAC activity by ~60% at 50 nM in PC3 lysates. At 4 μM, Jazz90 and Jazz167 increased acetylation in PC3 cells by 6- to 8-fold. Flow cytometry studies on the cell phase distribution demonstrated that Jazz90 causes a G0/G1 arrest in AR-null cells, whereas Jazz167 leads to a G0/G1 arrest in DU145 cells. However, apoptosis only occurred at a maximum of 7% of the total cell population following compound treatments in PC3 and DU145 cells. There was a reduction in cyclin D1 and no significant changes in bcl-2 in DU145 and PC3 cells. Overall, the results showed that Jazz90 and Jazz167 function as cytostatic HDAC inhibitors in AR-null prostate cancer cells

Charging Stations Distribution Optimization using Drones Fleet for Disaster Prone Areas

A disaster is an unforeseen calamity that causes damage to property or brings about a loss of human life. Quick response and rapid distribution of vital relief items into the affected region could save precious lives. In this regard, disaster management comes into play, which is highly dependent on the topography of the disaster-hit area. If the disaster-hit area has little or no road connectivity, the use of drones in such areas becomes essential for the delivery of health packages. Since the battery capacity of the drone is limited, there is a need of charging stations that should be transported using road infrastructure and pre-installed in disaster-prone areas, as access to these areas may be denied once the disaster hits. In this article, a simulation model was used to optimize the number and location of drone charging stations for deployment in a disaster-prone area in the pre-disaster scenario, aiming at the distribution of relief items to disaster-hit areas in the post-disaster scenario. We consider the relative priority of locations where a preference is given to the locations that have higher priority levels. An optimal number of charging stations and optimal routes have also been determined by using our optimization model. To illustrate the use of our model, numerical examples have been simulated for different sizes of the disaster-hit area and the number of targets. In our numerical simulation, it was observed that the drone's maximum distance capacity is the key factor in determining the optimal grid size, which directly correlates to the number of charging stations.

Charging Stations Distribution Optimization using Drones Fleet for Disaster Prone Areas

A disaster is an unforeseen calamity that causes damage to property or brings about a loss of human life. Quick response and rapid distribution of vital relief items into the affected region could save precious lives. In this regard, disaster management comes into play, which is highly dependent on the topography of the disaster-hit area. If the disaster-hit area has little or no road connectivity, the use of drones in such areas becomes essential for the delivery of health packages. Since the battery capacity of the drone is limited, there is a need of charging stations that should be transported using road infrastructure and pre-installed in disaster-prone areas, as access to these areas may be denied once the disaster hits. In this article, a simulation model was used to optimize the number and location of drone charging stations for deployment in a disaster-prone area in the pre-disaster scenario, aiming at the distribution of relief items to disaster-hit areas in the post-disaster scenario. We consider the relative priority of locations where a preference is given to the locations that have higher priority levels. An optimal number of charging stations and optimal routes have also been determined by using our optimization model. To illustrate the use of our model, numerical examples have been simulated for different sizes of the disaster-hit area and the number of targets. In our numerical simulation, it was observed that the drone's maximum distance capacity is the key factor in determining the optimal grid size, which directly correlates to the number of charging stations.

Antibiotic Effects of Loperamide: Homology of Human Targets of Loperamide with Targets in Acanthamoeba spp.

Background: Loperamide is an anti-diarrheal drug prescribed for non-infectious diarrhea. The drug is an opioid receptor agonist, blocker of voltage-dependent calcium channel (Cav) and calmodulin (CaM) inhibitor on human cells. Loperamide has been reported to exert anti-amoebic effects against pathogenic strains of Acanthamoeba castellanii. Objectives: The precise mode of antibiotic action, cellular target homology with human counterparts and the pattern of cell death induced by loperamide in Acanthamoeba castellanii remain to be established. Additionally, we attempt to establish the presence a primitive Cav in Acanthamoeba castellanii. Methods: Bioinformatics, 3D structural modelling, ligand binding predictions and apoptotic/ amoebicidal assays were used in this study to answer the above queries. Amino acid sequences and structural models were compared between human and A. castellanii proteins that are involved in the regulation of calcium (Ca+2) homeostasis. Results: Our results show that A. castellanii expresses similar, to near identical types of primitive calcium channels Cav Ac and CaM that are well known targets of loperamide in humans. The growth assays showed anti-amoebic effects of loperamide at different doses, both alone and in combinations with other Ca+2- CaM inhibitors. The synergistic actions of loperamide with haloperidol showed to be more amoebicidal than when either of them used alone. Imaging with Annexin V, Acridine orange and Propidium iodide showed apoptosis in A. castellanii at a dose of 100 µg/ml and necrosis at higher doses of 250 µg/ml. Conclusion: Though, Acanthamoeba does not express a homolog of the human mu-opioid receptor, but does shows evidence of the homologs for other known human targets of loperamide that are involved in Ca+2 uptake and Ca+2 signal transduction pathways. This suggests optimization of similar drug interactions with these targets may be useful in developing new approaches to control the growth of this parasite and possibly the diseases caused by it

Phytopharmacological potential of different species of Morus alba and their bioactive phytochemicals: A review

Medicinal plants of Moraceae family have been well-recognized traditionally due to their versatile applications in various fields including agriculture, cosmetic and food as well as in pharmaceutical industries. Their biomedical and medicinal importance is reflected from their broad range of pharmacological activities for treatment of various inflammatory conditions, cancer, infectious diseases, and gastrointestinal disorders. The present review was aimed to summarize and critically discuss the biomedical implications of Morus species, their bioactive compounds, and phytochemicals. Bioactivity guided fractionation of these medicinal plants revealed that different types of bioactive phytochemicals and secondary metabolites such as steroids, saponins, alkaloids, glycosides and phenolic compounds including terpenoids, flavonoids, anthocyanins and tannins were present. The critical analysis of the literature revealed that the aqueous, methanolic, and ethanolic extracts of Morus species and their bioactive compounds exhibit remarkable anti-oxidative, anti-diabetic, anti-stress, nephroprotective, antimicrobial, anti-mutagenic, anticancer, anxiolytic, hepatoprotective, anthelmintic, antimicrobial, immune-modulatory and cholesterol lowering effects. Based on the literature review and bioactivity guided investigation of Morus species and their phytomedicinal effects, we anticipate that these herbal products hold excellent potential for future research