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Not AvailableThis study was aimed to assess the effect of pre-freeze addition of cysteine hydrochloride and glutathione (GSH) on post-thaw sperm functional parameters and field fertility. The experimental bulls (n = 6) aged 4-6 years were used for this study. A total of 36 ejaculates (six ejaculates per bull) were collected and divided into three groups, group I (control), group II (5 mm cysteine hydrochloride) and group III (5 mm GSH). The extended semen samples with and without additives were filled in mini straw using automatic filling and sealing machine and cryopreserved in liquid nitrogen. Post-freeze seminal traits were recorded after thawing at 37°C for 30 s. The curvilinear velocity (VCL) and amplitude of lateral head displacement values were significantly (p < 0.05) higher in GSH than the cysteine and control groups. Post-thaw plasmalemma integrity was significantly (p < 0.01) higher in GSH group when compared to cysteine and control groups. The loss of acrosomal integrity was significantly (p < 0.01) lower in GSH than in cysteine and control groups. Addition of GSH non-significantly (p = 0.1) improved mitochondrial membrane potential (MMP) (per cent) than control group. This study indicates that the addition of cysteine and GSH suggestive (p = 0.08) of reducing lipid peroxide levels. The conception rate (%) in glutathione group was significantly (p < 0.05) higher than that in cysteine (68 vs 58) and control (49) groups. The post-thaw sperm progressive forward motility (r = 0.4) had moderate, but no significant correlation with conception rate. However, post-thaw straight-line velocity (r = 0.7), loss of acrosomal integrity (r = -0.8) and MMP (r = 0.9) had significant (p < 0.05) correlation with field fertility. This study indicates that the use of glutathione as semen additive may be used for improving post-thaw semen quality and overall augmentation of pregnancy in cows.Not Availabl

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Not AvailableAn experiment was carried out to study the electrophoretic profile of sperm membrane proteins in Assam Hill goat (AHG) bucks and their correlation with fresh semen characteristics. Ejaculates (64) consisting of 8 each from 8 bucks were collected by artificial vagina method. Each ejaculate was split into 2 equal parts. First part was used for fresh semen evaluation and the other part for protein study. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated 20 different protein bands with molecular weight ranging from 10 kDa to 75 kDa. Among the 20 proteins, only 6 proteins bands were consistently present in all 8 bucks (100%). Protein bands with molecular weight 10, 14, 22, 28, 55, 57 and 60 kDa showed significant positive correlation with certain parameters of the fresh semen, while 47 kDa protein band presented negative correlation with fresh semen characteristics in AHG bucks. In conclusion, the proteins showing significant positive correlation with fresh semen characteristics might serve to screen semen of AHG bucks for breeding programmes.Not Availabl