Cultivar differences in flowering response of okra (Abelmoschus esculentus) in spring and autumn

オクラの10系統と5品種を供試して,春季と秋季において花成反応の品種間差異を調査した。系統HE006, HE022, HE045, HE047, HE047-1と\u27Benny\u27, \u27Better Five\u27, \u27Clemson Spineless\u27, \u27Emerald\u27, \u27Green Rocket\u27の花芽は春季では2～6の低節位に,秋季には8～11節の低節位に形成された。秋季に比べて春季で品種間に差はあるが,出雷は0～11日早くおこった。季節に関わらず, HE015, HE050, HE050-1の花芽は播種後18～21日と早く形成されたが, HE025, HE035では32日～48日と遅く形成された。すべての系統と品種について,開花と結実時期は季節によって変動した。春季と秋季の環境条件に対する花成反応から,敏感な品種とそうでない品種に分けられた。敏感な品種にはHE006, HE022, HE045, HE047, HE047-1, \u27Benny\u27, \u27Better Five\u27, \u27Clemson Spineless\u27, \u27Emerald\u27と\u27Green Rocket\u27があった。敏感でない品種は2群に分けられ,花芽分化の早いのはHE015, HE050, HE050-1であり,遅いのはHE025, HE035であった。Differences in flowering response of ten accessions and five cultivars were investigated in spring and autumn. Flower buds of HE 006, HE 022, HE 045, HE 047, HE 047-1, \u27Benny\u27, \u27Better Five\u27, \u27Clemson Spineless\u27, \u27Emerald\u27 and \u27Green Rocket\u27 were initiated at the lower node from 2 to 6 in spring and from 8 to 11 in autumn and they appeared 0 to 11 days earlier in spring than in autumn, depending on the cultivars. Flower bud initiation of HE 015, HE 050 and HE 050-1 was early in 18 to 21 days after sowing (DAS) but the initiation of HE 025 and HE 035 was late in 32 to 48 DAS whether in spring or in autumn. The occurrence of anthesis and fruiting in all accessions and cultivars fluctuated in the different seasons. Based on these results, two groups of flowering response to environmental conditions in spring and autumn were identified as sensitive and non-sensitive cultivars. Sensitive cultivars comprised of HE 006, HE 022, HE 045, HE 047, HE 047-1, \u27Benny\u27, \u27Better Five\u27, \u27Clemson Spineless\u27, \u27Emerald\u27 and \u27Green Rocket\u27. Non-sensitive cultivars were divided into two subgroups as early and late flowering. Early flowering composed of HE 015, HE 050 and HE 050-1 and late flowering consisted of HE 025 and HE 035. (Accepted September 9, 2003

Morphological properties of flower and fruit in okra (Abelmoschus esculentus)

タイで栽培されるオクラの10系統と日本で栽培される20品種を供試して,花と果実の形態的特性を調査した。開花時には,どの系統・品種でもその外観はほぼ同じであった。果実は五角形あるいは丸形で,細長い形状をしていた。供試したすべての系統・品種は,Abelmoschus esculentusに属していた。10系統と20品種は,クラスター分析により3群に分類された。クラスター1には\u27Benny\u27, \u27Better Five\u27, \u27Blue Sky\u27, \u27Early Five\u27, \u27Emerald\u27, Green Etude\u27, Green Five\u27, Green Rocket\u27, Green Star\u27, Gulliver\u27, \u27Holiday\u27, \u27Miyako Gokaku\u27, \u27Natsu no Megumi\u27, \u27Peak Five\u27, \u27Pentagon\u27, \u27Star Debut\u27, \u27Star Light\u27, \u27Suisei Gokaku\u27, HE 015, HE 045, HE 047, HE 047-1とHE 050-1が含まれた。クラスター2には\u27Blue Sky Z\u27, \u27Clemson Spineless\u27, HE 006とHE 050が含まれた。クラスター3にはHE 022, HE 025とHE 035が含まれていた。Twenty okra cultivars from Japan and ten okra accessions from Thailand were cultivated to compare the morphological properties of flowers and fruits. Flower organs of all cultivars and accessions were quite similar in appearance at anthesis. The slender fruits with pentagon or round shaped were observed. All cultivars and accessions in this study belonged to Abelmoschus esculentus. Twenty cultivars and ten accessions were divided into 3 clusters. Cluster I consisted of \u27Benny\u27, \u27Better Five\u27, \u27Blue Sky\u27, \u27Early Five\u27, \u27Emerald\u27, \u27Green Etude\u27, \u27Green Five\u27, \u27Green Rocket\u27, \u27Green Star\u27, \u27Gulliver\u27, \u27Holiday\u27, \u27Miyako Gokaku\u27, \u27Natsu no Megumi\u27, Teak Five\u27, \u27Pentagon\u27, \u27Star Debut\u27, \u27Star Light\u27, \u27Suisei Gokaku\u27, HE 015, HE 045, HE 047, HE 047-1 and HE 050-1. Cluster II was composed of \u27Blue Sky Z\u27, \u27Clemson Spineless\u27, HE 006 and HE 050. Cluster III was comprised of HE 022, HE 025 and HE 035

Effect of Salicylic Acid Foliar Application on Bioactive Compounds and Antioxidant Activity in Holy Basil (Ocimum sanctum L.)

Holy basil (Ocimum sanctum L.) has been used extensively in Thai traditional medicine, where it is commonly utilized as a part of herbal remedies for treating various ailments. Cultivation methods using exogenous salicylic acid (SA) to induce secondary metabolites have been documented in various plant species. Nevertheless, there is no reported information available on holy basil. Thus, the present study aimed to investigate the impact of SA foliar application on the bioactive compounds and antioxidant activity of holy basil. SA at concentrations of 0.1, 0.5, 1.0, 1.5, 2.0, and 2.5 mM was foliar sprayed 30 days after transplanting (DAT) compared to spraying with tap water as the control. The plants were harvested at 33 DAT. Exogenous SA at 0.1–1.5 mM enhanced the contents of bioactive compounds and improved antioxidant activity. The highest contents of eugenol (17,829.53 ± 243.11 μg/g dry extract), total phenolics (444.10 ± 2.80 mg GAE/g dry extract), and total flavonoids (382.69 ± 6.49 mg QE/g dry extract) were achieved at 1.0 mM SA foliar application, which was 282.96, 1.76, and 2.14 times, respectively, over control. Furthermore, the greatest antioxidant activity was observed in the 1.0 mM SA treatment. In contrast, the 2.0 and 2.5 mM SA treatments had lower levels of antioxidant activity and bioactive compounds than the control. The results of this study suggest that exogenous 1.0 mM SA foliar application is an effective method to produce enriched bioactivity in holy basil

Enhancement of Plumbagin Production through Elicitation in In Vitro-Regenerated Shoots of <i>Plumbago indica</i> L.

Plumbago indica L. contains a valuable bioactive compound called plumbagin. Elicited regenerated shoots grown in vitro could be another source of high-yielding plumbagin. The purpose of this investigation was to examine the effects of elicitor type and concentration, as well as elicitation period, on plumbagin content in in vitro-regenerated shoots of P. indica. Nodal explants were cultured on Murashige and Skoog (MS) medium containing 1 mg/L benzyladenine (BA) in combination with 0–150 mg/L yeast extract or 50–150 µM salicylic acid for four weeks. Plumbagin levels of 3.88 ± 0.38% and 3.81 ± 0.37% w/w g dry extract were achieved from the 50 and 100 mg/L yeast extract-elicited shoots, which were higher than the value obtained for the control. However, the addition of salicylic acid did not increase the plumbagin content. In the elicitation period experiment, nodal explants were cultured on MS medium supplemented with 1 mg/L BA and 50 mg/L yeast extract for durations of three, four and five weeks. The 4-week yeast extract-elicited shoot had a maximum plumbagin content of 3.22 ± 0.12% w/w g dry extract, greater than that of the control. In summary, the plumbagin content of the in vitro P. indica shoots was enhanced by 4-week elicitation using 50 mg/L yeast extract