ANTIDIABETIC EFFECTS OF A SERIES OF CURCUMIN PYRAZOLES IN-VITRO

Objective: To investigate the antidiabetic potential of a series of curcumin pyrazole derivatives in-vitro.Methods: The in-vitro antidiabetic ability of curcumin pyrazole derivatives (3a-3e) was evaluated by the inhibition studies of the digestive enzymes;including alpha-amylase, rat intestinal alpha-glucosidase, and sucrase. The glucose lowering effect of the compounds was further assessed by theglucose uptake assay using a porcine diaphragm.Results: The curcumin pyrazole derivatives (3a-3e) showed a prominent inhibition of the enzymes studied. Compounds 3a and 3b were found tobe more pronounced in their hypoglycemic effects and 3b exhibited the maximum enzyme inhibitory activity. The glucose uptake through porcinediaphragm was extensively promoted by the compounds 3a and 3b than the other compounds in the series.Conclusion: The results suggest that the pyrazole derivatives of curcumin 3a and 3b have potent hypoglycemic properties. These compounds in theseries of curcumin pyrazole derivatives (3a-3e) can be selected for further in-vitro and in vivo studies.Keywords: Diaphragm, Insulin, Hypoglycemic, Enzyme inhibition

ANTAGONISTIC EFFECTS OF COMBINATION OF FLAXSEED OIL AND SPIRULINA PLATENSIS OIL ON THEIR BIOLOGICAL PROPERTIES

Objective: To determine the composition of flaxseed oil and Spirulina platensis oil and to evaluate their biological activities either alone or in combination by in-vitro studies.Methods: Proximate analysis of flaxseeds and S. platensis powder, followed by compositional analysis of oil by gas chromatography was conducted. Cytotoxic studies were performed by MTT and trypan blue dye exclusion assay. Hypoglycemic studies were assessed by glucose uptake assay using porcine diaphragm and by gluconeogenesis assay using rat liver slices. Anti-denaturation assay and HRBC membrane assay were conducted to evaluate the anti-inflammatory properties.Results: α-linolenic acid (ALA) was the predominant component of flaxseed oil while γ-linolenic acid was found only in S. platensis oil. In all the experiments, S. platensis oil and flaxseed oil alone exhibited prominent anticancer, antidiabetic and anti-inflammatory activities. Whereas, all the above activities were reduced in the combination studies irrespective of the ratio of the mixture of oils used.Conclusion: The results clearly suggested that the combination of S. platensis oil and flaxseed oil does not have any synergistic properties, indeed the combination of oils induced antagonistic effects on their biological properties.Â

IN VITRO ANTICANCER AND ANTI-LIPOXYGENASE ACTIVITIES OF CHIA SEED OIL AND ITS BLENDS WITH SELECTED VEGETABLE OILS

  Objective: To investigate the anticancer and anti-inflammatory potential of chia seed oil (CSO) and its blends in vitro.Methods: The in vitro cancer cytotoxic properties ability of CSO and its blends was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) and trypan blue assays using major cell lines including chronic myelogenous leukemia, HeLa and MCF-7 cells. The anti-inflammatory effect of the CSO and its blends was studied by assessing the anti-lipoxygenase (LOX) activity in vitro.Results: Incubation with CSO alone significantly inhibited the LOX activity. In the combination groups, CSO slightly increased the inhibition among blends of soybean and palmolein oils. In addition, CSO alone and in blends demonstrated potent and differential anticancer activity in MTT and trypan blue assays.Conclusion: Our results pave the way for the conclusion that CSO alone or in combination with the vegetable oils proves to be a healthy synergistic supplement or an adjuvant for the current diet. The supplementation with CSO is suggested for the modern lifestyle to delay or prevent the incidence of degenerative disorders

IN VITRO ANTICANCER AND ANTI-LIPOXYGENASE ACTIVITIES OF CHIA SEED OIL AND ITS BLENDS WITH SELECTED VEGETABLE OILS

  Objective: To investigate the anticancer and anti-inflammatory potential of chia seed oil (CSO) and its blends in vitro.Methods: The in vitro cancer cytotoxic properties ability of CSO and its blends was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) and trypan blue assays using major cell lines including chronic myelogenous leukemia, HeLa and MCF-7 cells. The anti-inflammatory effect of the CSO and its blends was studied by assessing the anti-lipoxygenase (LOX) activity in vitro.Results: Incubation with CSO alone significantly inhibited the LOX activity. In the combination groups, CSO slightly increased the inhibition among blends of soybean and palmolein oils. In addition, CSO alone and in blends demonstrated potent and differential anticancer activity in MTT and trypan blue assays.Conclusion: Our results pave the way for the conclusion that CSO alone or in combination with the vegetable oils proves to be a healthy synergistic supplement or an adjuvant for the current diet. The supplementation with CSO is suggested for the modern lifestyle to delay or prevent the incidence of degenerative disorders

ANTIOXIDANT AND HYPOGLYCEMIC EFFECTS OF CURCUMIN PYRAZOLE DERIVATIVES

Objective: To investigate the antioxidant properties of curcumin pyrazole derivatives using different in-vitro models and hypoglycemic potential by gluconeogenesis studies.Methods: Antioxidant ability of curcumin pyrazole derivatives was evaluated by using DPPH, nitric oxide, superoxide anion scavenging and lipid peroxidation assays comparing with standard, ascorbic acid (AA). The hypoglycemic effects of the compounds (3a-3e) were assessed by gluconeogenesis inhibition assay using rat liver slices comparing with standard, Insulin.Results: Compounds demonstrated strong scavenging activities against 2, 2-diphenyl, 2-picryl hydrazyl (DPPH), nitric oxide, superoxide anion radicals and also effectively inhibited lipid peroxidation. Compounds 3a, 3b and 3e exhibited significant activity in quenching DPPH, superoxide anion radical, nitric oxide and showed anti-lipid peroxidation. Other compounds 3c and 3d showed moderate activities. The gluconeogenesis inhibitory effects were more pronounced with compounds 3a and 3b compared to compounds 3c, 3d and 3e.Conclusion: Curcumin pyrazole derivatives showed considerable antioxidant activity against free radicals and lipid peroxidation. They exhibited significant IC50 values and thus can promote prominent protection against oxidative damage. The compounds 3a and 3b could be promising hypoglycemic agents as they are capable of lowering blood glucose by inhibiting gluconeogenesis and can be selected for further in-vitro and in vivo anti-diabetic investigations.Â