Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD 600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD 600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction

Agrobacterium rhizogenes Mediated Hairy Root Induction in Justicia gendarussa Burm.f

ABSTRACT Five strains of Agrobacterium rhizogenes (LB510, LB509, YMB072001, A4T, and ATCC 15834) were tested to determine their effects on the induction of hairy root on leaf explants of Justicia gendarussa Burm. f.. Leaf explants were sterilized, and then infected within liquid MS medium + sucrose containing A. rhizogenes OD600 = 0.1, for 20 minutes while being shaken gently by hand. Teh explant was co-cultivated on Murashige and Skoog (MS) solid medium at 25°C for 48 hours. After 48 hours of co- cultivation, explants were transferred to MS solid medium with the addition of 250 mg/L cefotaxim, and repeated 1-2 times. Observations were carried out during 6 weeks to observe the specified parameters. Data were analized by Kruskal-Wallis Test and followed by Mann-Whitney Test. All strains of A. rhizogenes were used in this study were able to produce hairy root. The treatment of LB510 and YMB072001 strain was the best strain in the hairy root induction of J. gendarussa Burm. f.. The infection frequency of LB510 strain was highest ( 100 % ), longest hairy root (2.23 cm), and highest of hairy root wet weight (0.0243 g). The treatment of YMB072001 strain was the highest number of hairy roots (3.3) per explant and the highest number of hairy roots dry weight (0.0098 g)

Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be  available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction. </p

EFEK FARMAKOLOGIS DAUN MENIRAN (PHYLLANTHUS NIRURI LINN.) TERHADAP OTOT POLOS USUS HALUS SECARA TERPISAH PADA KELINCI

Kandungan meniran yang telah banyak diketahui adalah zat pahit (phillantine) yang telah dapat diisolasi, tergolong alkaloid, sebagaimana kandungan alkaloid pada spesies phyllanthus yang lain. Selain itu masih terdapat sejumlah zat inorganik serta beberapa zat lain yang belum diketahui. yang dibuktikan dengan adanya kadar abu yang sebagian larut dan sebagian tidak larut dalam asam.Penelitian ini bcrtujuan untuk mengetahui efek farmakologis daun meniran (Phyllanthus niruri Linn.) terhadap otot polos usus halus terpisah pada kelinci, dan dari hasil penelitian ini diharapkan dapat memberikan informasi kepada masyarakat tentang penggunaan daun meniran sebagai obat antidiare

Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be  available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction. </p