7 research outputs found
Electrophysiological Phenotype Characterization of Human iPSCâDerived Neuronal Cell Lines by Means of HighâDensity Microelectrode Arrays
Recent advances in the field of cellular reprogramming have opened a route to studying the fundamental mechanisms underlying common neurological disorders. Highâdensity microelectrodeâarrays (HDâMEAs) provide unprecedented means to study neuronal physiology at different scales, ranging from network through singleâneuron to subcellular features. In this work, HDâMEAs are used in vitro to characterize and compare human inducedâpluripotentâstemâcellâderived dopaminergic and motor neurons, including isogenic neuronal lines modeling Parkinson's disease and amyotrophic lateral sclerosis. Reproducible electrophysiological network, singleâcell and subcellular metrics are used for phenotype characterization and drug testing. Metrics, such as burst shape and axonal velocity, enable the distinction of healthy and diseased neurons. The HDâMEA metrics can also be used to detect the effects of dosing the drug retigabine to human motor neurons. Finally, it is shown that the ability to detect drug effects and the observed cultureâtoâculture variability critically depend on the number of available recording electrodes. © 2021 Wiley-VCHISSN:2701-019