EFFICACY OF ULTRASOUND WITH MAITLAND MOBILIZATION OVER SHORT WAVE DIATHERMY WITH MAITLAND MOBILIZATION IN IMPROVING THE FUNCTIONAL PERFORMANCE OF PATIENTS WITH PERIARTHRITIS SHOULDER

Objective: To investigate the effectiveness of ultrasound with Maitland mobilization over Short wave diathermy with Maitland mobilization in improving the functional performance of patients with Periarthritis of shoulder Design: A simple randomized controlled clinical trail Setting: The study was conducted in the department of physiotherapy in Ganga Hospital Coimbatore (India), Vinayaka Mission Hospital Salem (India). Subjects: 30 patients were selected randomly from the population using simple random sampling procedure (Lottery Method) and were divided into two equal groups. Intervention : The experimental group (n=15) were given Ultrasound with Maitland mobilization with 1 MHz in frequency, continuous mode and 1.5 W/cm 2 of intensity with 5cm 2 sized transducer for 10 minutes of treatment duration .The control group (n=15) were given Short wave diathermy with Maitland mobilization for period of 15 min with contra planar technique. Outcome measures: The functional performance was measured using Shoulder Pain and Disability Index (SPADI) scale. Results: In Group-A (Experimental Group ) and Group-B (Control Group), all data was expressed as mean ± , SD and was statistically analysed using paired 't' test and independent 't' test to determine the statistical difference among the parameters at 0.5% level of significance. Statistical data of SPADI showed that, Group-A is significantly different from Group-B with p<0.05; i.e 95% of significance. Conclusion: The post ultrasound Maitland mobilization is found more effective and beneficial than post SWD Maitland mobilization on shoulder functions in periarthritis. KEYWORDS: Maitland mobilizations, Short wave diathermy, Ultrasound, Periarthritis, Glides. Quick Response cod

IMPURITY PROFILING IMPURITY PROFILING OF THIAMINE HYDROCHLORIDE INJECTION BY RP-HPLC AND CHARACTERIZATION OF DEGRADATION PRODUCT BY LC-MS/MS/QTOF

Objective: To propose a comprehensive, simple, and affordable RP-HPLC method for impurity profiling and characterization of unknown degradation products of thiamine hydrochloride injectable formulation. Methods: The chromatographic separation employs gradient mode using the octadecyl silane column using a mobile phase consisting of phosphate buffer with ion pair reagent, acetonitrile, and methanol delivered flow rate at 1.2 ml/min. The detection was carried out at 248 nm using empower software. LC-MS/MS/QTOF hyphenated technique was used for isolation and characterization of unknown degradation impurity. The performance of the method was systematically validated as per ICH Q2 (R1) guidelines. Results: Degradation product observed in accelerated stability was characterized by LC-MS/MS/QTOF hyphenated technique and found m/z value 351.1604 and postulated as an oxidative degradation product of thiamine due to excipient interaction. The validated method was sensitive, selective, and specific data proves the method is precise and accurate from LOQ to 150% level and results are within 95-108% and less than 4.5% RSD. The developed method is linear from 0.03-58.83 µg/ml with a correlation coefficient of more than 0.990 and LOD and LOQ value ranged from 0.03 to1.51 μg/ml. Conclusion: An efficient RP-HPLC method for impurity profiling of thiamine injectable formulation was successfully developed and unknown degradation product observed instability condition samples characterized by LC-MS/MS/QTOF technique. The validated method can be successfully employed for the impurity profiling of thiamine injectable in the quality control department